Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,657,212	C→A	G575V (GGC→GTC)	hscA ←	iron‑sulfur cluster biosynthesis chaperone HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,657,212	0	C	A	100.0%	267.7 / NA	76	G575V (GGC→GTC)	hscA	iron‑sulfur cluster biosynthesis chaperone HscA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/36);  total (40/36)

CTTTGGCATATTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGCCCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTG  >  NC_000913/2657075‑2657347                                                                                                                                          |                                                                                                                                        cTTTGGCATATTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGGTGGCGTCAACATCATCGacc                                                                                                                                       <  2:1534576/140‑1 (MQ=255)   ttGGCATATTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTg                                                                                                                                     <  1:954561/140‑1 (MQ=255)    tGGCATATTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTgc                                                                                                                                    >  1:1310965/1‑140 (MQ=255)        atatTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCa                                                                                                                                <  1:1296443/140‑1 (MQ=255)          atTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACt                                                                                                                              >  1:1656477/1‑140 (MQ=255)             aaaCCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCa                                                                                                                           <  2:1310965/140‑1 (MQ=255)                ccTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTc                                                                                                                        >  2:1572185/1‑140 (MQ=255)                   cGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAgg                                                                                                                     >  1:1113431/1‑140 (MQ=255)                   cGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAgg                                                                                                                     >  1:356708/1‑140 (MQ=255)                   cGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAgg                                                                                                                     >  1:1377917/1‑140 (MQ=255)                    gTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGt                                                                                                                    >  1:1710893/1‑140 (MQ=255)                    gTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGt                                                                                                                    >  1:707886/1‑140 (MQ=255)                    gTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGt                                                                                                                    >  1:666002/1‑140 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTc                                                                                                                        <  2:1822203/135‑1 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTc                                                                                                                        <  2:595306/135‑1 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTc                                                                                                                        >  1:1822203/1‑135 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTc                                                                                                                        >  1:595306/1‑135 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTg                                                                                                                   >  2:1505432/1‑140 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTg                                                                                                                   >  2:1445424/1‑140 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTg                                                                                                                   >  1:76749/1‑140 (MQ=255)                     tCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTg                                                                                                                   >  1:808561/1‑140 (MQ=255)                            aaTGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGgca                                                                                                            <  1:1829044/140‑1 (MQ=255)                               ggCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGgcagca                                                                                                         <  1:1680595/140‑1 (MQ=255)                               ggCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGgcagca                                                                                                         >  1:895934/1‑140 (MQ=255)                                gCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCAt                                                                                                        <  2:1710893/140‑1 (MQ=255)                                gCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCAt                                                                                                        <  2:1807298/140‑1 (MQ=255)                                gCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCAt                                                                                                        <  1:1445424/140‑1 (MQ=255)                                     tCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCa                                                                                                   >  1:218808/1‑140 (MQ=255)                                      cAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCaa                                                                                                  >  1:464516/1‑140 (MQ=255)                                      cAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCaa                                                                                                  >  1:748856/1‑140 (MQ=255)                                      cAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCaa                                                                                                  >  2:1812372/1‑140 (MQ=255)                                        gcgcACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATg                                                                                                <  1:143514/140‑1 (MQ=255)                                        gcgcACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATg                                                                                                <  2:847018/140‑1 (MQ=255)                                          gcACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGAc                                                                                              <  2:666002/140‑1 (MQ=255)                                           cACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAAt                                                                                                 >  2:926321/1‑136 (MQ=255)                                           cACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAAt                                                                                                 <  1:926321/136‑1 (MQ=255)                                             cgacgaACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTg                                                                                           <  2:488022/140‑1 (MQ=255)                                             cgacgaACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTg                                                                                           <  1:704471/140‑1 (MQ=255)                                             cgacgaACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTg                                                                                           <  2:76749/140‑1 (MQ=255)                                              gacgaACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGa                                                                                          >  1:1595207/1‑140 (MQ=255)                                                   aCCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTc                                                                                     >  1:586908/1‑140 (MQ=255)                                                     cGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTg                                                                                   <  2:1377917/140‑1 (MQ=255)                                                      gACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTgc                                                                                  <  1:1640022/140‑1 (MQ=255)                                                       aCTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTgcg                                                                                 <  1:1812372/140‑1 (MQ=255)                                                        cTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTgcgg                                                                                >  2:814431/1‑140 (MQ=255)                                                        cTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTgcgg                                                                                >  2:1219159/1‑140 (MQ=255)                                                        cTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTgcgg                                                                                >  2:1799435/1‑140 (MQ=255)                                                          ggTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTgcggcg                                                                              <  2:1113431/140‑1 (MQ=255)                                                              cATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTa                                                                          <  1:506510/140‑1 (MQ=255)                                                                tGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAAc                                                                        >  2:830907/1‑140 (MQ=255)                                                                     cgagcggcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAgcgc                                                                   >  1:472867/1‑140 (MQ=255)                                                                     cgagcggcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAgcgc                                                                   >  2:1589104/1‑140 (MQ=255)                                                                     cgagcggcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAgcgc                                                                   >  1:205250/1‑140 (MQ=255)                                                                       agcggcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCgg                                                                 >  2:144092/1‑140 (MQ=255)                                                                       agcggcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCgg                                                                 <  1:1799435/140‑1 (MQ=255)                                                                         cggcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCa                                                               <  2:808561/140‑1 (MQ=255)                                                                           gcgaAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATc                                                             >  1:61649/1‑140 (MQ=255)                                                                              aaaTCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATcagc                                                          <  2:895934/140‑1 (MQ=255)                                                                                       gtttgtttGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAgcgc                                                 <  2:707886/140‑1 (MQ=255)                                                                                            tttgtCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGt                                            <  2:205250/140‑1 (MQ=255)                                                                                                 cTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGa                                       <  2:218808/140‑1 (MQ=255)                                                                                                  tACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGAc                                      >  2:1329148/1‑140 (MQ=255)                                                                                                  tACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGAc                                      <  2:1178941/140‑1 (MQ=255)                                                                                                     gTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACttt                                   >  2:24159/1‑140 (MQ=255)                                                                                                          tAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAgcgcg                                                <  1:89228/122‑1 (MQ=255)                                                                                                          tAATCGCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAgcgcg                                                >  2:89228/1‑122 (MQ=255)                                                                                                               gCTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACAcg                         >  1:1223970/1‑140 (MQ=255)                                                                                                                cTTGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACAcgc                        >  2:943002/1‑140 (MQ=255)                                                                                                                  tGTTCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACgcgc                      <  2:464516/140‑1 (MQ=255)                                                                                                                    ttCGATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGcgccg                    <  2:1505366/140‑1 (MQ=255)                                                                                                                       gATGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCtt                 <  2:1223970/140‑1 (MQ=255)                                                                                                                         tGGCGTCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCt               <  1:1572185/140‑1 (MQ=255)                                                                                                                              tCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCt               >  2:624719/1‑135 (MQ=255)                                                                                                                              tCAACATCATCGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCt               <  1:624719/135‑1 (MQ=255)                                                                                                                                   atcatcGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGtt     <  1:1546979/140‑1 (MQ=255)                                                                                                                                     catcGACCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCt   >  2:733880/1‑140 (MQ=255)                                                                                                                                      atcGACCTGCGCCACTGCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGTCGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTg  >  2:434806/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CTTTGGCATATTAAACCTCGTCCACGGAATGGCCTTTCAGCGCACGACGAACCGACTGGTCCATGCGGCGAGCGGCGAAATCCTGGGTTTGTTTGTCTACGTTTTTAATCGCTTGTTCGATGGCGTCAACATCATCGCCCTGCGCCACTTCACTCAGGTGAGCGGCAGCATCGTCAATGACCTGACGTTCTGCGGCGCTTAACAGCGCGGCATCAGCAGCCAGCGCGCCGTGCAGACTTTCCAGCACACGCGCCGCTTCTACTTTTTGTTCTG  >  NC_000913/2657075‑2657347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑