Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,548,380 C→A G564V (GGC→GTC)  malQ ← 4‑alpha‑glucanotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,548,3800CA98.5% 470.7 / ‑1.1 136G564V (GGC→GTC) malQ4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (78/56);  total (79/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 140 total aligned reads displayed.

ACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCA  >  NC_000913/3548243‑3548515
                                                                                                                                         |                                                                                                                                       
aCGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGacc                                                                                                                                       <  2:1441810/140‑1 (MQ=255)
  gATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAg                                                                                                                                     <  2:112400/140‑1 (MQ=255)
     gCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAg                                                                                                                                     <  1:223356/137‑1 (MQ=255)
     gCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAg                                                                                                                                     >  2:223356/1‑137 (MQ=255)
               cGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCCGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCg                                                                                                                        <  2:1578267/140‑1 (MQ=255)
               cGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCg                                                                                                                        <  1:77074/140‑1 (MQ=255)
                ggCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGc                                                                                                                       >  2:277378/1‑140 (MQ=255)
                 gCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGc                                                                                                                       <  2:940769/139‑1 (MQ=255)
                 gCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGc                                                                                                                       >  1:940769/1‑139 (MQ=255)
                 gCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCa                                                                                                                      <  1:978913/140‑1 (MQ=255)
                  cACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCAc                                                                                                                     >  1:288985/1‑140 (MQ=255)
                       ttCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCg                                                                                                                        <  2:1168249/132‑1 (MQ=255)
                               aCAACCATATTTATGCAGTGCATCCAGGAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACcgc                                                                                                        >  2:642251/1‑140 (MQ=255)
                                 aaCCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCg                                                                                                                        >  2:1694858/1‑122 (MQ=255)
                                 aaCCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCg                                                                                                                        <  1:1694858/122‑1 (MQ=255)
                                 aaCCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCgcgc                                                                                                      <  2:1659977/140‑1 (MQ=255)
                                           aTGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTTACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCa                                                                                            >  1:313790/1‑140 (MQ=255)
                                            tGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAg                                                                                           >  1:477676/1‑140 (MQ=255)
                                             gCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTTATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAgg                                                                                          >  1:1681218/1‑140 (MQ=255)
                                             gCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAgg                                                                                          >  2:1241298/1‑140 (MQ=255)
                                             gCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCCCAGCGTTggaggg                                                                                          >  1:432638/1‑136 (MQ=255)
                                              cAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGt                                                                                         >  1:1610581/1‑140 (MQ=255)
                                               aGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTc                                                                                        <  2:477676/140‑1 (MQ=255)
                                                gTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCa                                                                                       >  1:804293/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  2:1131666/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  1:950211/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  2:1754165/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  2:406450/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  2:431795/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  1:1034131/1‑140 (MQ=255)
                                                 tGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCAATCCCAGTAACCGCGCAGCGTTGGCAGGTCAt                                                                                      >  1:923457/1‑140 (MQ=255)
                                                  gCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAtg                                                                                     >  2:1680899/1‑140 (MQ=255)
                                                  gCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAtg                                                                                     >  2:432832/1‑140 (MQ=255)
                                                   cATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGc                                                                                             <  2:1714686/131‑1 (MQ=255)
                                                   cATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGc                                                                                             >  1:1714686/1‑131 (MQ=255)
                                                   cATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCAtgt                                                                                    >  2:614739/1‑140 (MQ=255)
                                                         gcagcCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTcgcc                                                                              >  2:1283208/1‑140 (MQ=255)
                                                           agcCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCTAGTAACCGCGCAGCGTTGGCAGGTCATGTGTcgccgc                                                                            >  2:629163/1‑140 (MQ=255)
                                                               cTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAAcc                                                                        >  2:918832/1‑140 (MQ=255)
                                                               cTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAAcc                                                                        >  2:686026/1‑140 (MQ=255)
                                                                ttGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCg                                                                       <  1:1283208/140‑1 (MQ=255)
                                                                        ccAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCTAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGAc                                                               <  1:629163/140‑1 (MQ=255)
                                                                        ccAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGAc                                                               <  2:13424/140‑1 (MQ=255)
                                                                           gTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGc                                                            <  1:520863/140‑1 (MQ=255)
                                                                            ttCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCt                                                           <  1:667823/140‑1 (MQ=255)
                                                                            ttCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGATGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCt                                                           <  2:950211/140‑1 (MQ=255)
                                                                              cgcgATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTcc                                                         >  2:1419224/1‑140 (MQ=255)
                                                                                   tCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATa                                                    <  1:918832/140‑1 (MQ=255)
                                                                                        aTACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCtt                                               >  2:63185/1‑140 (MQ=255)
                                                                                        aTACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCtt                                               <  2:288985/140‑1 (MQ=255)
                                                                                             gACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGt                                          >  1:1778751/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                         >  2:751890/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                         >  1:1152361/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                         >  1:227971/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                         >  2:746953/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                         >  1:912986/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTg                                         >  1:1518250/1‑140 (MQ=255)
                                                                                              aCCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTCTCGGTg                                         >  1:708829/1‑140 (MQ=255)
                                                                                               ccGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGc                                        >  1:1238427/1‑140 (MQ=255)
                                                                                               ccGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGc                                        <  1:751890/140‑1 (MQ=255)
                                                                                                 gcgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCAc                                      <  2:1238427/140‑1 (MQ=255)
                                                                                                 gcgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCAc                                      <  1:686026/140‑1 (MQ=255)
                                                                                                  cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                     >  2:917882/1‑140 (MQ=255)
                                                                                                  cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                     <  2:612239/140‑1 (MQ=255)
                                                                                                  cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                     >  1:1088473/1‑140 (MQ=255)
                                                                                                  cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                     >  2:1572460/1‑140 (MQ=255)
                                                                                                  cgcAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACg                                     >  1:47564/1‑140 (MQ=255)
                                                                                                    cAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                   <  1:250475/140‑1 (MQ=255)
                                                                                                    cAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                   <  2:1033196/140‑1 (MQ=255)
                                                                                                    cAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                   <  1:183098/140‑1 (MQ=255)
                                                                                                         ccACTTCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGtc                              <  1:553921/140‑1 (MQ=255)
                                                                                                             ttCATCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGtcttct                          >  1:347008/1‑140 (MQ=255)
                                                                                                                 tCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                   >  2:1303801/1‑127 (MQ=255)
                                                                                                                 tCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGa                                   <  1:1303801/127‑1 (MQ=255)
                                                                                                                 tCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                      >  1:807453/1‑140 (MQ=255)
                                                                                                                 tCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                      >  1:92520/1‑140 (MQ=255)
                                                                                                                 tCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                      >  1:1482213/1‑140 (MQ=255)
                                                                                                                 tCCGGATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTg                      >  1:162063/1‑140 (MQ=255)
                                                                                                                    ggATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTc                   <  2:1152361/140‑1 (MQ=255)
                                                                                                                     gATACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCg                  >  2:1007748/1‑140 (MQ=255)
                                                                                                                       tACAGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGtt                >  1:91721/1‑140 (MQ=255)
                                                                                                                          aGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTc             >  2:343536/1‑140 (MQ=255)
                                                                                                                          aGCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTc             >  2:67539/1‑140 (MQ=255)
                                                                                                                           gCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCg            <  1:63185/140‑1 (MQ=255)
                                                                                                                           gCCCCAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCg            >  2:742655/1‑140 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCGGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCg            <  2:196531/139‑1 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGa           >  2:877586/1‑140 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGa           >  2:854272/1‑140 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGa           >  1:601132/1‑140 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGa           >  1:149839/1‑140 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGa           >  2:1808971/1‑140 (MQ=255)
                                                                                                                            ccccAGGGTTTTGACCAGCGTTAGATCCCCGCACCCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGa           >  2:301182/1‑140 (MQ=255)
                                                                                                                                aGGGTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATa       <  1:1241298/140‑1 (MQ=255)
                                                                                                                                 gggTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATag      <  1:1220134/140‑1 (MQ=255)
                                                                                                                                   gTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag    <  1:431795/140‑1 (MQ=255)
                                                                                                                                   gTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag    <  1:1131666/140‑1 (MQ=255)
                                                                                                                                   gTTTTGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATagag    <  2:1124812/140‑1 (MQ=255)
                                                                                                                                     tttGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCa  <  1:877586/140‑1 (MQ=255)
                                                                                                                                     tttGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCa  <  2:92520/140‑1 (MQ=255)
                                                                                                                                     tttGACCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCa  <  1:1007748/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCAGTTCGCGATCCTGATACAGACCGCGCAGTACCACTTCATCCGGATACAGCCCCAGGGTTTTGCCCAGCGTTAGATCCCCGCACTCCCAGTAACCGCGCAGCGTTGGCAGGTCATGTGTCGCCGCAACCGCCATCGACTGCTCCGGATACGCTTTCGGTGCACGGAACGTCTTCTCGTGGTCGTTTTCGAAATAGAGCA  >  NC_000913/3548243‑3548515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑