Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,522	G→T	P24T (CCT→ACT)	yobF ←	DUF2527 domain‑containing protein YobF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,522	0	G	T	100.0%	336.7 / NA	94	P24T (CCT→ACT)	yobF	DUF2527 domain‑containing protein YobF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (50/44);  total (50/44)

AAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCC  >  NC_000913/1907385‑1907656                                                                                                                                          |                                                                                                                                       aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCt                                                                                                                                      >  1:1425530/1‑140 (MQ=255)  aaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGtctc                                                                                                                                     <  2:124216/140‑1 (MQ=255)         tAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCagag                                                                                                                              <  1:1781867/140‑1 (MQ=255)         tAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCagag                                                                                                                              <  1:925710/140‑1 (MQ=255)            aCTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCGGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAg                                                                                                                           <  1:1231660/140‑1 (MQ=255)            aCTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAg                                                                                                                           <  1:78382/140‑1 (MQ=255)                gTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGt                                                                                                                       >  2:2055151/1‑140 (MQ=255)                gTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGt                                                                                                                       >  1:1193618/1‑140 (MQ=255)                 ttGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTa                                                                                                                      <  2:1425530/140‑1 (MQ=255)                   gAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTAtt                                                                                                                    <  1:2055151/140‑1 (MQ=255)                        aCTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATtcaacg                                                                                                               >  1:1430481/1‑140 (MQ=255)                         cTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATtcaacgt                                                                                                              >  2:758935/1‑140 (MQ=255)                          ttAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCagag                                                                                                                              <  1:1827936/123‑1 (MQ=255)                          ttAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCagag                                                                                                                              >  2:1827936/1‑123 (MQ=255)                          ttAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATtcaacgtc                                                                                                             <  2:1430481/140‑1 (MQ=255)                          ttAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATtcaacgtc                                                                                                             <  1:930618/140‑1 (MQ=255)                            aaCCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATtcaacgtcaa                                                                                                           <  1:1798104/140‑1 (MQ=255)                                 gACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACgtgt                                                                                                      <  2:967762/140‑1 (MQ=255)                                  aCCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGtt                                                                                                     >  2:2091468/1‑140 (MQ=255)                                  aCCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATACATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGACAACGTGtt                                                                                                     >  2:2378609/1‑140 (MQ=255)                                           cTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGa                                                                                            <  1:1117413/140‑1 (MQ=255)                                              tGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGActt                                                                                         >  1:204249/1‑140 (MQ=255)                                              tGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGActt                                                                                         >  2:1080062/1‑140 (MQ=255)                                               gCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGActtc                                                                                        >  1:82339/1‑140 (MQ=255)                                                ccATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGActtct                                                                                       <  1:519297/140‑1 (MQ=255)                                                             tCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGc                                                                          <  2:1802388/140‑1 (MQ=255)                                                              ccTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGcc                                                                         >  2:1164887/1‑140 (MQ=255)                                                                         tttCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCca                                                                        >  1:234140/1‑130 (MQ=255)                                                                         tttCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACa                                                              <  1:1080062/140‑1 (MQ=255)                                                                         tttCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGAca                                                                        <  2:234140/130‑1 (MQ=255)                                                                          ttcttcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                                                                <  1:114089/137‑1 (MQ=255)                                                                          ttcttcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                                                                >  2:114089/1‑137 (MQ=255)                                                                            cttcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGaa                                                           <  2:1548474/140‑1 (MQ=255)                                                                              tcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACt                                                         <  2:1397124/140‑1 (MQ=255)                                                                              tcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACt                                                         >  1:1057018/1‑140 (MQ=255)                                                                              tcGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACt                                                         >  1:1397124/1‑140 (MQ=255)                                                                               cGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTg                                                        >  1:1142908/1‑140 (MQ=255)                                                                                  ccGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAAc                                                               >  1:692761/1‑130 (MQ=255)                                                                                  ccGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAAc                                                               <  2:692761/130‑1 (MQ=255)                                                                                  ccGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTAc                                                     >  1:1026251/1‑140 (MQ=255)                                                                                  ccGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTAc                                                     <  2:1142908/140‑1 (MQ=255)                                                                                       ggCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGt                                                <  2:1057018/140‑1 (MQ=255)                                                                                         cATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGttt                                              <  1:2041643/140‑1 (MQ=255)                                                                                          aTAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTa                                             >  1:978306/1‑140 (MQ=255)                                                                                            aaCATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAAc                                           >  1:1109482/1‑140 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTAc                                                     <  2:427023/124‑1 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTAc                                                     >  1:427023/1‑124 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGaaa                                     >  2:2515132/1‑140 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGaaa                                     >  2:293673/1‑140 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGaaa                                     >  2:1014459/1‑140 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGaaa                                     >  2:1101466/1‑140 (MQ=255)                                                                                                  gATAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGaaa                                     >  1:14935/1‑140 (MQ=255)                                                                                                   aTAAAACTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAAt                                    >  2:1324692/1‑140 (MQ=255)                                                                                                      aaaCTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTg                                 <  1:293673/140‑1 (MQ=255)                                                                                                       aaCTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGt                                >  2:1126578/1‑140 (MQ=255)                                                                                                        aCTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGtt                               >  2:901758/1‑140 (MQ=255)                                                                                                        aCTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGtt                               <  2:82339/140‑1 (MQ=255)                                                                                                        aCTGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGtt                               <  1:1324692/140‑1 (MQ=255)                                                                                                          tGACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTAt                             <  2:978306/140‑1 (MQ=255)                                                                                                           gACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATc                            >  2:46918/1‑140 (MQ=255)                                                                                                           gACACATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATc                            <  1:1126578/140‑1 (MQ=255)                                                                                                            acacATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATca                           >  2:2629143/1‑140 (MQ=255)                                                                                                            acacATTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATca                           >  2:2125578/1‑140 (MQ=255)                                                                                                             cataTTACTGCATGAGGCACCAATATAAGTCTCGGCGGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATcac                          >  2:1809665/4‑140 (MQ=255)                                                                                                             cacaTTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGGACTGTACCTCGTTTAACCCGAAATc                                   <  2:2139615/131‑1 (MQ=255)                                                                                                             cacaTTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGGACTGTACCTCGTTTAACCCGAAATc                                   >  1:2139615/1‑131 (MQ=255)                                                                                                             cacaTTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACATCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATcac                          >  2:199821/1‑140 (MQ=255)                                                                                                                aTTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       <  1:46918/140‑1 (MQ=255)                                                                                                                aTTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       <  1:901758/140‑1 (MQ=255)                                                                                                                aTTACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       <  1:2515132/140‑1 (MQ=255)                                                                                                                 ttACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATAc                      <  1:1164887/140‑1 (MQ=255)                                                                                                                 ttACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATAc                      <  1:2125578/140‑1 (MQ=255)                                                                                                                 ttACTGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATAc                      <  2:1109482/140‑1 (MQ=255)                                                                                                                     tGCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg                  <  1:2378609/140‑1 (MQ=255)                                                                                                                      gCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATAc                      <  2:505275/135‑1 (MQ=255)                                                                                                                      gCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATAc                      >  1:505275/1‑135 (MQ=255)                                                                                                                      gCATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGt                 >  2:1925708/1‑140 (MQ=255)                                                                                                                       cATGAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGtt                >  1:285493/1‑140 (MQ=255)                                                                                                                          gAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       >  1:1316576/1‑130 (MQ=255)                                                                                                                          gAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       >  2:1151505/1‑130 (MQ=255)                                                                                                                          gAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       <  1:1151505/130‑1 (MQ=255)                                                                                                                          gAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATa                       <  2:1316576/130‑1 (MQ=255)                                                                                                                          gAGGCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAAt             >  1:2429603/1‑140 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACTTAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTa           >  2:2467650/1‑140 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg                  <  1:598285/133‑1 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACg                  >  2:598285/1‑133 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTa           >  2:2488350/1‑140 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTa           >  1:177142/1‑140 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTa           >  2:714824/1‑140 (MQ=255)                                                                                                                            ggCACCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTa           >  2:2195999/1‑140 (MQ=255)                                                                                                                               aCCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATgg        >  2:2527935/1‑140 (MQ=255)                                                                                                                               aCCAATATAAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATgg        >  1:1102903/1‑140 (MQ=255)                                                                                                                                    tataAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc   <  2:1193618/140‑1 (MQ=255)                                                                                                                                    tataAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc   <  2:1102903/140‑1 (MQ=255)                                                                                                                                     ataAGTCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGcc  <  1:661009/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCC  >  NC_000913/1907385‑1907656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑