BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,218	G→T	S393R (AGC→AGA)	parE ←	DNA topoisomerase IV subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,218	0	G	T	100.0%	638.9 / NA	179	S393R (AGC→AGA)	parE	DNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (94/85); total (94/85)

Only 100 of 182 total aligned reads displayed.

CATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTCACCACGC  >  NC_000913/3174083‑3174354

cATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTgg                                                                                                                                      >  2:600111/1‑140 (MQ=255)

 aTGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGt                                                                                                                                     <  2:2372932/140‑1 (MQ=255)

 aTGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGt                                                                                                                                     <  1:835701/140‑1 (MQ=255)

 aTGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGt                                                                                                                                     <  2:2519938/140‑1 (MQ=255)

  tGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTc                                                                                                                                    >  1:337074/1‑140 (MQ=255)

   gATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCa                                                                                                                                   <  2:83625/140‑1 (MQ=255)

     tCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGc                                                                                                                                 >  1:470896/1‑140 (MQ=255)

      cGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCt                                                                                                                                <  2:1598879/140‑1 (MQ=255)

        ccTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCttt                                                                                                                              >  1:1022421/1‑140 (MQ=255)

          tGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCttttt                                                                                                                            >  1:2613740/1‑140 (MQ=255)

          tGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCttttt                                                                                                                            >  1:231818/1‑140 (MQ=255)

           gATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTa                                                                                                                           >  1:247174/1‑140 (MQ=255)

              atTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGc                                                                                                                        >  2:439616/1‑140 (MQ=255)

                 cgcgATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGcacc                                                                                                                     >  2:1962108/1‑140 (MQ=255)

                 cgcgATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGcacc                                                                                                                     >  1:1400797/1‑140 (MQ=255)

                   cgATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGcaccac                                                                                                                   >  1:2559979/1‑140 (MQ=255)

                      tCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACttt                                                                                                                >  1:1974302/1‑140 (MQ=255)

                       cgcgcgCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGcacc                                                                                                                     >  2:2548246/1‑134 (MQ=255)

                       cgcgcgCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACtttt                                                                                                               >  2:2412796/1‑140 (MQ=255)

                       cgcgcgCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACtttt                                                                                                               >  1:2220207/1‑140 (MQ=255)

                       cgcgcgCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACtttt                                                                                                               >  1:2501256/1‑140 (MQ=255)

                          gcgcCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTg                                                                                                            <  2:2471743/140‑1 (MQ=255)

                            gcCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGc                                                                                                          <  1:1713625/140‑1 (MQ=255)

                            gcCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGc                                                                                                          <  1:685909/140‑1 (MQ=255)

                            gcCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGc                                                                                                          >  2:685909/1‑140 (MQ=255)

                                gCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCTTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCcgca                                                                                                      <  2:231818/140‑1 (MQ=255)

                                gCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGTcgca                                                                                                      <  1:300291/140‑1 (MQ=255)

                                gCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCcgca                                                                                                      <  2:2613740/140‑1 (MQ=255)

                                 cTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCcgcac                                                                                                     <  1:183340/140‑1 (MQ=255)

                                    ggCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCcgcacgca                                                                                                  >  1:1759633/1‑140 (MQ=255)

                                      cAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATa                                                                                                <  2:1101896/140‑1 (MQ=255)

                                             gCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGTGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgct                                                                                         <  1:53637/140‑1 (MQ=255)

                                             gCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgct                                                                                         <  1:1160739/140‑1 (MQ=255)

                                             gCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgct                                                                                         <  2:337074/140‑1 (MQ=255)

                                             gCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgct                                                                                         <  2:1822275/140‑1 (MQ=255)

                                              ccTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgctg                                                                                        >  1:2590976/1‑140 (MQ=255)

                                                tGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgctggg                                                                                      >  1:873285/1‑140 (MQ=255)

                                                tGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACggcgctggg                                                                                      <  1:1962108/140‑1 (MQ=255)

                                                       tCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGa                                                                               >  1:2228076/1‑140 (MQ=255)

                                                       tCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCAC‑TTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGaa                                                                              <  2:544563/140‑1 (MQ=255)

                                                        cACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGaa                                                                              <  1:2412796/140‑1 (MQ=255)

                                                            ttCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCg                                                                          <  2:1286300/140‑1 (MQ=255)

                                                               cacaAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCa                                                                       <  2:1759633/140‑1 (MQ=255)

                                                                     gAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCg                                                                 >  2:625715/1‑140 (MQ=255)

                                                                     gAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCg                                                                 >  1:2507370/1‑140 (MQ=255)

                                                                        cAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTcc                                                                  <  1:2056015/136‑1 (MQ=255)

                                                                         aGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCcag                                                             <  2:1400797/140‑1 (MQ=255)

                                                                          gCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCg                                                                 <  2:843927/135‑1 (MQ=255)

                                                                          gCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCg                                                                 >  1:843927/1‑135 (MQ=255)

                                                                            tCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCcagcag                                                          >  1:941687/1‑140 (MQ=255)

                                                                             cGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGt                                                         <  2:2222832/140‑1 (MQ=255)

                                                                                tACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCa                                                      <  2:1868240/140‑1 (MQ=255)

                                                                                tACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCa                                                      <  1:439616/140‑1 (MQ=255)

                                                                                tACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCa                                                      >  1:1868240/1‑140 (MQ=255)

                                                                                tACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCAGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCa                                                      >  2:1253196/1‑140 (MQ=255)

                                                                                  cGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAgc                                                    <  1:2174911/140‑1 (MQ=255)

                                                                                     ttatggTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAgccgc                                                 >  2:1983447/5‑140 (MQ=255)

                                                                                     ttAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAgccgc                                                 >  1:2172651/1‑140 (MQ=255)

                                                                                       aaGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCt                                               <  1:1010326/140‑1 (MQ=255)

                                                                                         ggTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGa                                             <  2:2184155/140‑1 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAgccgc                                                 <  2:1502613/135‑1 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAgccgc                                                 >  1:1502613/1‑135 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGaa                                            <  2:941687/140‑1 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGaa                                            <  2:873285/140‑1 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGaa                                            >  2:704176/1‑140 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGaa                                            >  1:1328285/1‑140 (MQ=255)

                                                                                          gTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGaa                                            <  1:926889/140‑1 (MQ=255)

                                                                                             cTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGt                                         >  2:315013/1‑140 (MQ=255)

                                                                                              tGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGtt                                        >  2:173849/1‑140 (MQ=255)

                                                                                              tGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGtt                                        >  1:810594/1‑140 (MQ=255)

                                                                                              tGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGtt                                        >  1:899693/1‑140 (MQ=255)

                                                                                              tGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGtt                                        <  2:810594/140‑1 (MQ=255)

                                                                                               gcgcGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGa                                             <  2:771865/134‑1 (MQ=255)

                                                                                               gcgcGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGa                                             >  1:771865/1‑134 (MQ=255)

                                                                                               gcgcGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTc                                       >  2:1140257/1‑140 (MQ=255)

                                                                                                cgcgGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCt                                      <  2:2590976/140‑1 (MQ=255)

                                                                                                    gTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGtt                                  <  1:560023/140‑1 (MQ=255)

                                                                                                        aaTCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGc                              <  1:805694/140‑1 (MQ=255)

                                                                                                          tCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCca                            <  2:781345/140‑1 (MQ=255)

                                                                                                          tCAGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCca                            <  2:899693/140‑1 (MQ=255)

                                                                                                          tCAGCCAGTTTGCCAGGCAACGCCAGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCca                            <  1:1253196/140‑1 (MQ=255)

                                                                                                            aGCCAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCcac                           <  1:272427/139‑1 (MQ=255)

                                                                                                              ccAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGt                                   <  1:263496/129‑1 (MQ=255)

                                                                                                              ccAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGt                                   >  2:263496/1‑129 (MQ=255)

                                                                                                              ccAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAgg                        >  2:1523918/1‑140 (MQ=255)

                                                                                                               cAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGa                       <  1:625715/140‑1 (MQ=255)

                                                                                                               cAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGa                       >  1:1503909/1‑140 (MQ=255)

                                                                                                               cAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGa                       >  1:1312219/1‑140 (MQ=255)

                                                                                                               cAGTTTGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGa                       >  2:2042280/1‑140 (MQ=255)

                                                                                                                  tttGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATaa                    >  1:2243079/1‑140 (MQ=255)

                                                                                                                  tttGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATaa                    >  2:2126001/1‑140 (MQ=255)

                                                                                                                   ttGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCca                            <  1:1123256/131‑1 (MQ=255)

                                                                                                                    tGCCAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAg                  >  2:2290602/1‑140 (MQ=255)

                                                                                                                      ccAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGc                >  1:1693628/1‑140 (MQ=255)

                                                                                                                      ccAGGCAACGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGc                <  2:1312219/140‑1 (MQ=255)

                                                                                                                              cGCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTca        >  1:2542572/1‑140 (MQ=255)

                                                                                                                               gCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGTGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTcac       >  2:398995/1‑140 (MQ=255)

                                                                                                                               gCCGGGCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTcac       <  1:1550575/140‑1 (MQ=255)

                                                                                                                                    gCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTCACCACGc  <  2:2507370/140‑1 (MQ=255)

                                                                                                                                    gCCTCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTCACCACGc  <  2:2174186/140‑1 (MQ=255)

CATGATCGCCTGATATTCGCGATCGCGCGCCTGCTTGGCAGATCCGCCTGCGGAGTCACCTTCCACAAGGAACAGCTCGGTACGGTTAAGGTCCTGCGCGGTACAATCAGCCAGTTTGCCAGGCAACGCCGGGCCGCTGGTCAGCTTTTTACGCACCACTTTTTTGGCCGCACGCATACGGCGCTGGGCGCTGGAAATCGCCATCTCCGCCAGCAGTTCAGCCGCCTGAACGTTCTGGTTCAGCCACAGGATAAAGGCATCTTTCACCACGC  >  NC_000913/3174083‑3174354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`