Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,666,866	C→A	D239Y (GAC→TAC)	gadA ←	glutamate decarboxylase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,666,866	0	C	A	100.0%	221.6 / NA	88	D239Y (GAC→TAC)	gadA	glutamate decarboxylase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (50/38);  total (50/38)

CCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCATG  >  NC_000913/3666743‑3667002                                                                                                                            |                                                                                                                                         ccAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGgtg                                                                                                                          >  2:1221767/1‑140 (MQ=11)  cAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGgtgt                                                                                                                         <  2:1271668/140‑1 (MQ=14)       cGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGcc                                                                                                                    <  2:581769/140‑1 (MQ=17)        gAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCt                                                                                                                   <  2:400024/140‑1 (MQ=17)         aaTTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTg                                                                                                                  >  1:59858/1‑140 (MQ=17)               tGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAAttt                                                                                                            >  1:635927/1‑140 (MQ=17)                 gCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTAt                                                                                                          <  2:2153430/140‑1 (MQ=18)                    tGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCa                                                                                                       >  1:2090001/1‑140 (MQ=17)                      aaGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAgc                                                                                                     <  2:635927/140‑1 (MQ=18)                         cACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCa                                                                                                  <  2:1678592/140‑1 (MQ=18)                         cACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCa                                                                                                  <  2:2351304/140‑1 (MQ=18)                         cACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCa                                                                                                  <  2:1056699/140‑1 (MQ=18)                            tGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCg                                                                                               >  1:2238132/1‑140 (MQ=17)                            tGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCg                                                                                               <  2:576046/140‑1 (MQ=18)                              aTCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTg                                                                                             >  1:135505/1‑140 (MQ=17)                              aTCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTg                                                                                             <  1:1221767/140‑1 (MQ=17)                                cGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCa                                                                                           >  1:524633/1‑140 (MQ=17)                                   tttCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCa                                                                                                  >  1:2027082/1‑130 (MQ=17)                                   tttCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCa                                                                                                  <  2:2027082/130‑1 (MQ=18)                                   tttCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCg                                                                                        <  2:59858/140‑1 (MQ=18)                                      cacaCGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCa                                                                                           >  1:636934/1‑134 (MQ=17)                                      cacaCGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCa                                                                                           <  2:636934/134‑1 (MQ=18)                                      cacaCGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGtt                                                                                     >  2:507974/1‑140 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTtgtg                                                                                  <  2:1474264/136‑1 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTtgtg                                                                                  >  1:1474264/1‑136 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGaa                                                                              >  1:2121748/1‑140 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGaa                                                                              >  2:2079234/1‑140 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGaa                                                                              >  2:1245021/1‑140 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGaa                                                                              >  1:1304395/1‑140 (MQ=17)                                             ggCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGaa                                                                              >  1:820404/1‑140 (MQ=17)                                              gCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAAc                                                                             <  1:938235/140‑1 (MQ=17)                                                       aGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTa                                                                    >  1:1457015/1‑140 (MQ=17)                                                       aGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTa                                                                    >  2:1276597/1‑140 (MQ=17)                                                        gTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGACCTCATAGTTAc                                                                   <  2:2383057/140‑1 (MQ=17)                                                        gTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc                                                                   <  1:1245021/140‑1 (MQ=17)                                                        gTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc                                                                   <  1:1765715/140‑1 (MQ=17)                                                        gTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc                                                                   <  2:820404/140‑1 (MQ=17)                                                        gTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAc                                                                   <  2:135505/140‑1 (MQ=17)                                                         tCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTAcc                                                                  >  1:958227/1‑140 (MQ=17)                                                            cAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGgt                                                               >  2:2087057/1‑140 (MQ=21)                                                            cAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGgt                                                               >  1:70689/1‑140 (MQ=21)                                                                   tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTc                                                        >  2:1631410/1‑140 (MQ=21)                                                                   tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTc                                                        >  1:2225246/1‑140 (MQ=21)                                                                    atCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCa                                                       <  2:2272840/140‑1 (MQ=25)                                                                    atCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCa                                                       >  1:241660/1‑140 (MQ=21)                                                                    atCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCa                                                       >  2:2346921/1‑140 (MQ=21)                                                                         gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCg                                                  >  1:1221975/1‑140 (MQ=21)                                                                         gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCg                                                  >  1:1018024/1‑140 (MQ=21)                                                                         gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCg                                                  >  1:2400313/1‑140 (MQ=21)                                                                         gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCg                                                  <  2:1221975/140‑1 (MQ=25)                                                                           gCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGaa                                                >  1:511959/1‑140 (MQ=21)                                                                             gacgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAg                                              >  1:78812/1‑140 (MQ=21)                                                                              acgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGt                                             >  1:16884/1‑140 (MQ=21)                                                                               cgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGa                                                 >  1:711557/1‑135 (MQ=21)                                                                               cgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGa                                                 <  2:711557/135‑1 (MQ=25)                                                                                 aaCGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCgg                                          >  1:2627077/1‑140 (MQ=21)                                                                                    ggTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGcac                                       <  2:524633/140‑1 (MQ=21)                                                                                    ggTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGcac                                       <  2:2225246/140‑1 (MQ=25)                                                                                     gTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGcacc                                      >  1:66788/1‑140 (MQ=21)                                                                                      tGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGcacca                                     <  2:241660/140‑1 (MQ=21)                                                                                            gAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAGCTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGa                               <  2:78812/140‑1 (MQ=21)                                                                                            gAAGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGa                               <  2:1304395/140‑1 (MQ=21)                                                                                              aGCCACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATg                             <  1:1276597/140‑1 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  1:201009/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  1:190868/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  2:2178441/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  1:2030537/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  1:548306/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  1:70262/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  1:1559953/1‑140 (MQ=21)                                                                                                ccACCGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                           >  2:2445259/1‑140 (MQ=21)                                                                                                   ccGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGtt                        >  1:1930353/1‑140 (MQ=21)                                                                                                    cGCTGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGttt                       >  2:2412881/1‑140 (MQ=21)                                                                                                       tGGCAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCg                    >  2:1237999/1‑140 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCTTAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  1:507974/140‑1 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  1:2178441/140‑1 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  1:261750/140‑1 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  2:548306/140‑1 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  1:2445259/140‑1 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  2:2121748/140‑1 (MQ=21)                                                                                                          cAGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca                 <  2:66788/140‑1 (MQ=21)                                                                                                           aGCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGCcac                <  1:769089/140‑1 (MQ=19)                                                                                                            gCGTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTcaca               <  2:2400313/140‑1 (MQ=21)                                                                                                              gTCGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAgg             >  2:2013770/1‑140 (MQ=21)                                                                                                                cGATGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCt           >  1:2586519/1‑140 (MQ=25)                                                                                                                  aTGTGCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTc         >  2:834929/1‑140 (MQ=21)                                                                                                                     tgCATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAAt      >  2:1767214/1‑140 (MQ=25)                                                                                                                       cATGTAGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCa    >  1:923938/1‑140 (MQ=21)                                                                                                                         tgtagatgtcgaTACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCATg  >  1:1533138/1‑140 (MQ=21)                                                                                                                         tgtagatgtcgaTACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCATg  <  1:2087057/140‑1 (MQ=21)                                                                                                                            |                                                                                                                                         CCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTGTCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCGGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCATG  >  NC_000913/3666743‑3667002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑