Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,916,862 A→G S172P (TCC→CCC)  atpD ← ATP synthase F1 complex subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,916,8620AG98.7% 552.1 / ‑2.9 154S172P (TCC→CCC) atpDATP synthase F1 complex subunit beta
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (78/74);  total (79/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 154 total aligned reads displayed.

CCCGGCGGCTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGAGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGC  >  NC_000913/3916732‑3916998
                                                                                                                                  |                                                                                                                                        
 ccGGCGGCTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATc                                                                                                                                >  2:412785/1‑140 (MQ=255)
    gcggcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATcgcg                                                                                                                             <  2:2164855/140‑1 (MQ=255)
     cggcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGa                                                                                                                            <  2:2062607/140‑1 (MQ=255)
     cggcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGa                                                                                                                            <  1:2224166/140‑1 (MQ=255)
      ggcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTg                                                                                                                                       >  2:1856539/1‑128 (MQ=255)
      ggcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTg                                                                                                                                       <  1:1856539/128‑1 (MQ=255)
       gcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCt                                                                                                                                     <  2:898924/129‑1 (MQ=255)
       gcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATg                                                                                                                          >  1:1041012/1‑140 (MQ=255)
       gcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGAGTGCt                                                                                                                                     >  1:1056098/1‑129 (MQ=255)
       gcTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGAGTGCt                                                                                                                                     <  2:1056098/129‑1 (MQ=255)
       gcTCGTTCATCTGGCCATACACCAGGGATACTTTATCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATg                                                                                                                          >  2:94513/1‑140 (MQ=255)
             tCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGa                                                                                                                    <  1:104267/140‑1 (MQ=255)
             tCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGa                                                                                                                    <  1:2623447/140‑1 (MQ=255)
               aTCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAAt                                                                                                                  <  2:1803837/140‑1 (MQ=255)
                tCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATg                                                                                                                 <  1:2304034/140‑1 (MQ=255)
                 cTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGa                                                                                                                <  1:2266060/140‑1 (MQ=255)
                  tGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAg                                                                                                               <  2:1041012/140‑1 (MQ=255)
                   ggCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAAt                                                                                                                  >  2:1903175/1‑136 (MQ=255)
                    gCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTa                                                                                                                       <  2:1408608/130‑1 (MQ=255)
                    gCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTa                                                                                                                       <  2:1158207/130‑1 (MQ=255)
                    gCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTa                                                                                                                       >  1:1408608/1‑130 (MQ=255)
                    gCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCt                                                                                                             <  2:2105455/140‑1 (MQ=255)
                    gCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCt                                                                                                             >  1:37426/1‑140 (MQ=255)
                      cATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGa                                                                                                                >  1:668702/1‑135 (MQ=255)
                         acacCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAAt                                                                                                                  >  1:2238459/1‑130 (MQ=255)
                         acacCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAAt                                                                                                                  <  2:2238459/130‑1 (MQ=255)
                         acacCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCcatc                                                                                                        <  1:919789/140‑1 (MQ=255)
                              aGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCcatc                                                                                                        >  1:1594857/1‑135 (MQ=255)
                              aGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCcatc                                                                                                        <  2:1594857/135‑1 (MQ=255)
                               gggATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGttt                                                                                                  <  1:529028/140‑1 (MQ=255)
                               gggATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGttt                                                                                                  >  2:720424/1‑140 (MQ=255)
                                    aCTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGt                                                                                             >  2:1599961/1‑140 (MQ=255)
                                    aCTTTGTCGATAACGTTGGAGTCGGTCATTCCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGt                                                                                             >  2:459259/1‑140 (MQ=255)
                                     cTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtt                                                                                            >  2:1437119/1‑140 (MQ=255)
                                     cTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtt                                                                                            <  1:522894/140‑1 (MQ=255)
                                     cTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtt                                                                                            >  2:795927/1‑140 (MQ=255)
                                      tttGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGttt                                                                                           <  1:2126911/140‑1 (MQ=255)
                                       ttGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtttt                                                                                          >  1:583077/1‑140 (MQ=255)
                                       ttGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtttt                                                                                          >  2:2497433/1‑140 (MQ=255)
                                        tGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTa                                                                                         >  1:1218172/1‑140 (MQ=255)
                                          tCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGttt                                                                                                  <  2:693849/129‑1 (MQ=255)
                                          tCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGttt                                                                                                  >  1:693849/1‑129 (MQ=255)
                                          tCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtt                                                                                            <  2:1478638/135‑1 (MQ=255)
                                          tCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtt                                                                                            <  2:2246502/135‑1 (MQ=255)
                                          tCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGtt                                                                                            >  1:1478638/1‑135 (MQ=255)
                                          tCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTAcc                                                                                       >  1:1257990/1‑140 (MQ=255)
                                           cGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCt                                                                                      <  1:159361/140‑1 (MQ=255)
                                               aaCGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTa                                                                                                 <  1:436218/125‑1 (MQ=255)
                                               aaCGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTa                                                                                                 >  2:436218/1‑125 (MQ=255)
                                                  gTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCg                                                                               <  2:583077/140‑1 (MQ=255)
                                                  gTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCg                                                                               <  1:459259/140‑1 (MQ=255)
                                                  gTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCg                                                                               <  1:946444/140‑1 (MQ=255)
                                                  gTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCg                                                                               <  1:2020990/140‑1 (MQ=255)
                                                     ggAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGcac                                                                            <  2:750612/140‑1 (MQ=255)
                                                           ggTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGa                                                                      >  2:1001844/1‑140 (MQ=255)
                                                           ggTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCCGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGa                                                                      >  2:134962/1‑140 (MQ=255)
                                                            gTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTATGGTTTTACCTACACCCGcacca                                                                          <  1:789010/135‑1 (MQ=255)
                                                            gTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGaa                                                                     >  2:264656/1‑140 (MQ=255)
                                                            gTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGaa                                                                     >  2:2063042/1‑140 (MQ=255)
                                                            gTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGaa                                                                     >  2:769711/1‑140 (MQ=255)
                                                                 ttCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGAc                                                                <  1:1001844/140‑1 (MQ=255)
                                                                          gAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTAc                                                       <  2:1257990/140‑1 (MQ=255)
                                                                             gTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGc                                                    >  2:1369867/1‑140 (MQ=255)
                                                                               cGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGccc                                                  >  1:2210633/1‑140 (MQ=255)
                                                                                gTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCt                                                 >  2:124309/1‑140 (MQ=255)
                                                                                  tACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTGCGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTa                                               >  2:1982805/1‑140 (MQ=255)
                                                                                      cTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGa                                           <  2:865549/140‑1 (MQ=255)
                                                                                      cTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGa                                           <  1:2393219/140‑1 (MQ=255)
                                                                                      cTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGa                                           >  1:2434953/1‑140 (MQ=255)
                                                                                      cTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGa                                           <  1:1369867/140‑1 (MQ=255)
                                                                                        cACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAAc                                         >  1:2437096/1‑140 (MQ=255)
                                                                                        cACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAAc                                         >  1:491655/1‑140 (MQ=255)
                                                                                        cACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAAc                                         >  2:1473866/1‑140 (MQ=255)
                                                                                        cACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAAc                                         >  1:2364641/1‑140 (MQ=255)
                                                                                         aCGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACg                                        >  1:1343574/1‑140 (MQ=255)
                                                                                           gAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGa                                      <  1:1215119/140‑1 (MQ=255)
                                                                                              tACGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGa                                      >  2:331657/1‑137 (MQ=255)
                                                                                                cGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGa                                           >  1:1291722/1‑130 (MQ=255)
                                                                                                cGTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGa                                           <  2:1291722/130‑1 (MQ=255)
                                                                                                 gTTCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATc                                <  2:2210633/140‑1 (MQ=255)
                                                                                                   tCACCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAg                              >  1:84158/1‑140 (MQ=255)
                                                                                                     aCCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGt                            >  2:2321964/1‑140 (MQ=255)
                                                                                                     aCCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGt                            >  2:2122616/1‑140 (MQ=255)
                                                                                                     aCCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGt                            >  1:375263/1‑140 (MQ=255)
                                                                                                     aCCTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGt                            >  2:52705/1‑140 (MQ=255)
                                                                                                       cTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCg                          >  1:1049262/1‑140 (MQ=255)
                                                                                                       cTACGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCg                          >  1:61493/1‑140 (MQ=255)
                                                                                                         aCGCCCGCAAACACAGAGTATCCGGGGTGCTCGATCGCGATGTTGCGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGAt                        <  1:1982805/140‑1 (MQ=255)
                                                                                                         aCGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGAt                        <  2:84158/140‑1 (MQ=255)
                                                                                                          cGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGAt                        <  1:1862113/139‑1 (MQ=255)
                                                                                                          cGCCCGCAAACACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATa                       >  1:2581332/1‑140 (MQ=255)
                                                                                                                 aaaCACAGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGa                >  2:2505189/1‑140 (MQ=255)
                                                                                                                    cacaGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATAc             <  2:1336684/140‑1 (MQ=255)
                                                                                                                      caGAGTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCg           <  1:2122660/140‑1 (MQ=255)
                                                                                                                       agagTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATAc             <  1:1449440/137‑1 (MQ=255)
                                                                                                                       agagTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATAc             >  2:1449440/1‑137 (MQ=255)
                                                                                                                         agTAACCGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGtt        <  2:2364641/140‑1 (MQ=255)
                                                                                                                              ccGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCcag   >  1:899839/1‑140 (MQ=255)
                                                                                                                               cGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCcatc  >  2:2065923/1‑138 (MQ=255)
                                                                                                                               cGGGGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCcagc  >  1:101665/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                        
CCCGGCGGCTCGTTCATCTGGCCATACACCAGGGATACTTTGTCGATAACGTTGGAGTCGGTCATTTCGTGGTAGAAGTCGTTACCCTCACGAGTACGTTCACCTACGCCCGCAAACACAGAGTAACCGGAGTGCTCGATCGCGATGTTACGAATGAGCTCCATCATGTTTACGGTTTTACCTACACCCGCACCACCGAACAGACCAACTTTACCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGC  >  NC_000913/3916732‑3916998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑