Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,542,254	G→T	R87S (CGC→AGC)	cysU ←	sulfate/thiosulfate ABC transporter inner membrane subunit CysU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,542,254	0	G	T	100.0%	340.6 / NA	96	R87S (CGC→AGC)	cysU	sulfate/thiosulfate ABC transporter inner membrane subunit CysU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/49);  total (47/49)

AAACTTCGCCAGCCATTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCGGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCAT  >  NC_000913/2542117‑2542389                                                                                                                                          |                                                                                                                                        aaaCTTCGCCAGCCATTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCtgg                                                                                                                                       >  1:80119/1‑140 (MQ=255)   aCTTCGCCAGCCATTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGtt                                                                                                                                     >  1:854728/1‑140 (MQ=255)    cTTCGCCAGCCATTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTa                                                                                                                                    <  2:90927/140‑1 (MQ=255)       cGCCAGCCATTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGa                                                                                                                                 >  2:2271930/1‑140 (MQ=255)         ccagccaTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATc                                                                                                                               <  2:1607369/140‑1 (MQ=255)         ccagccaTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATc                                                                                                                               <  2:1312021/140‑1 (MQ=255)          cagccaTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              <  2:1335555/140‑1 (MQ=255)          cagccaTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              <  2:2854835/140‑1 (MQ=255)          cagccaTTCACCATAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              <  1:2714027/140‑1 (MQ=255)             ccaTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGtt                                                                                                                                     >  1:2719007/1‑130 (MQ=255)             ccaTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGtt                                                                                                                                     <  2:2719007/130‑1 (MQ=255)              caTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              >  1:2865652/1‑136 (MQ=255)              caTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              <  2:2865652/136‑1 (MQ=255)              caTTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGc                                                                                                                          <  2:2184376/140‑1 (MQ=255)                 tCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTAGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCAt                                                                                                                       >  2:1576121/1‑140 (MQ=255)                  cACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATc                                                                                                                      >  1:2563909/1‑140 (MQ=255)                       tAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              <  2:115740/127‑1 (MQ=255)                       tAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATcc                                                                                                                              >  1:115740/1‑127 (MQ=255)                       tAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATcagcag                                                                                                                 <  2:1394754/140‑1 (MQ=255)                       tAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATcagcag                                                                                                                 >  2:748603/1‑140 (MQ=255)                       tAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATcagcag                                                                                                                 >  1:1846715/1‑140 (MQ=255)                               gTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGaaaa                                                                                                         >  2:1652412/1‑140 (MQ=255)                                 ttACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACg                                                                                                       >  2:834033/1‑140 (MQ=255)                                        aaaGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTaa                                                                                                >  2:1773793/1‑140 (MQ=255)                                                aGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGAt                                                                                        <  1:2814744/140‑1 (MQ=255)                                                 ggCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATg                                                                                       >  2:1104685/1‑140 (MQ=255)                                                 ggCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATg                                                                                       >  1:2288655/1‑140 (MQ=255)                                                  gCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACg                                                                                                       <  2:314342/123‑1 (MQ=255)                                                  gCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACg                                                                                                       >  1:314342/1‑123 (MQ=255)                                                  gCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGc                                                                                      <  1:1660624/140‑1 (MQ=255)                                                    cAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCca                                                                                    <  1:2271930/140‑1 (MQ=255)                                                      gCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCcaca                                                                                  >  1:1730342/1‑140 (MQ=255)                                                       cGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACaa                                                                                 <  1:1104685/140‑1 (MQ=255)                                                       cGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGAAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACaa                                                                                 <  2:2748890/140‑1 (MQ=255)                                                          tAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACg                                                                              <  1:748603/140‑1 (MQ=255)                                                             aCCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCg                                                                           >  2:1558323/1‑140 (MQ=255)                                                             aCCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCg                                                                           >  2:1567336/1‑140 (MQ=255)                                                                 gCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGAcag                                                                       >  1:2229392/1‑140 (MQ=255)                                                                 gCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGAcag                                                                       >  1:2576899/1‑140 (MQ=255)                                                                  cGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGAcagc                                                                      >  1:1796149/1‑140 (MQ=255)                                                                    aCAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGAcagcag                                                                    <  2:2576899/140‑1 (MQ=255)                                                                       gCCGTTGGCAGCGCAAAGGGTAAATCCTTCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGt                                                                 <  1:1452171/140‑1 (MQ=255)                                                                        ccGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGtt                                                                <  2:2106820/140‑1 (MQ=255)                                                                        ccGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGtt                                                                <  2:164397/140‑1 (MQ=255)                                                                        ccGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGtt                                                                <  1:1652412/140‑1 (MQ=255)                                                                         cGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTa                                                               >  2:1053327/1‑140 (MQ=255)                                                                               cAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGt                                                         <  1:1301028/140‑1 (MQ=255)                                                                                       aGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCcgcg                                                 >  2:2583076/1‑140 (MQ=255)                                                                                        gggTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGa                                                <  2:758128/140‑1 (MQ=255)                                                                                        gggTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCATTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGa                                                <  2:2675120/140‑1 (MQ=255)                                                                                         ggTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGac                                               <  2:2267815/140‑1 (MQ=255)                                                                                            aaaTCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGaccac                                            <  1:834033/140‑1 (MQ=255)                                                                                            aaaTCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGaccac                                            <  1:719927/140‑1 (MQ=255)                                                                                            aaaTCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGaccac                                            <  2:1846715/140‑1 (MQ=255)                                                                                              aTCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCt                                          <  1:1558323/140‑1 (MQ=255)                                                                                                 cATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCg                                       <  1:1503129/140‑1 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGTAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  1:1217684/1‑140 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  1:2176280/1‑140 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  2:954849/1‑140 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  2:712345/1‑140 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  1:575888/1‑140 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  2:656734/1‑140 (MQ=255)                                                                                                   tCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCggg                                     >  1:2403502/1‑140 (MQ=255)                                                                                                    cAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGt                                    >  2:2176632/1‑140 (MQ=255)                                                                                                        gcATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGt                                <  2:1730342/140‑1 (MQ=255)                                                                                                         cATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTg                               >  1:1055014/1‑140 (MQ=255)                                                                                                         cATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTg                               >  2:1432296/1‑140 (MQ=255)                                                                                                         cATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTg                               >  1:560179/1‑140 (MQ=255)                                                                                                          aTCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGa                              >  1:1909509/1‑140 (MQ=255)                                                                                                           tCAAGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGAt                             <  2:2563909/140‑1 (MQ=255)                                                                                                             aaGCGGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCa                           <  1:1153930/140‑1 (MQ=255)                                                                                                             aaGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCa                           <  2:1796149/140‑1 (MQ=255)                                                                                                             aaGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCa                           <  1:2176632/140‑1 (MQ=255)                                                                                                             aaGCAGCGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCa                           <  1:1567336/140‑1 (MQ=255)                                                                                                              agcagcGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCAc                          >  1:1958951/1‑140 (MQ=255)                                                                                                                 agcGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTc                       >  2:692797/1‑140 (MQ=255)                                                                                                                   cGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTg                                  <  1:1054174/127‑1 (MQ=255)                                                                                                                   cGTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTg                                  >  2:1054174/1‑127 (MQ=255)                                                                                                                    gTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTg                                  <  2:1197123/126‑1 (MQ=255)                                                                                                                    gTGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTg                                  >  1:1197123/1‑126 (MQ=255)                                                                                                                     tGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAg                   >  2:825393/1‑140 (MQ=255)                                                                                                                     tGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAg                   >  1:2236786/1‑140 (MQ=255)                                                                                                                     tGCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAg                   >  2:1356161/1‑140 (MQ=255)                                                                                                                      gCGGCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGt                  >  1:171178/1‑140 (MQ=255)                                                                                                                         gCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACt               <  1:1356161/140‑1 (MQ=255)                                                                                                                         gCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACt               <  1:721621/140‑1 (MQ=255)                                                                                                                         gCCTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACt               >  1:32076/1‑140 (MQ=255)                                                                                                                          ccTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGt                                <  1:2620520/122‑1 (MQ=255)                                                                                                                          ccTGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGt                                >  2:2620520/1‑122 (MQ=255)                                                                                                                            tGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGTAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTgcg            <  2:1217684/140‑1 (MQ=255)                                                                                                                            tGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTgcg            <  1:1773793/140‑1 (MQ=255)                                                                                                                            tGGGAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTgcg            <  2:854728/140‑1 (MQ=255)                                                                                                                              ggAAGCGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGcc          <  1:1432296/140‑1 (MQ=255)                                                                                                                                   cGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCt     >  2:2553890/1‑140 (MQ=255)                                                                                                                                   cGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCt     >  2:2546674/1‑140 (MQ=255)                                                                                                                                   cGATAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCt     <  2:1958951/140‑1 (MQ=255)                                                                                                                                      tAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCAt  <  1:692797/140‑1 (MQ=255)                                                                                                                                      tAGCTGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACCCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCAt  >  2:1374884/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AAACTTCGCCAGCCATTCACCGTAAAAACCGTTTACGGAAAAGAGCGAGGCCAGCGTTAAACCGGCGACAGCCGTTGGCAGCGCAAAGGGTAAATCCATCAGCGCATCAAGCAGCGTGCGGCCTGGGAAGCGATAGCGGGTTAGGATCCACGCCATCAGCAGACCGAAAACGCCGTTAAAAATCGATGCCACAAACGCCGACAGCAGCGTTACTTTGTAGGCCGCGACCACCTGCGGGTTGGTGATCACCTCCCAGTACTGCGCCCAGCTCAT  >  NC_000913/2542117‑2542389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑