Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,5930TG25.6% 287.2 / 25.4 125K12N (AAA→AAC) iclRDNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base T (13/80);  new base G (32/0);  total (45/80)
Fisher's exact test for biased strand distribution p-value = 1.18e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 146 total aligned reads displayed.

GGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCA  >  NC_000913/4223455‑4223732
                                                                                                                                          |                                                                                                                                           
ggAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCATGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGtt                                                                                                                                            >  1:2486477/1‑140 (MQ=255)
    ttGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCt                                                                                                                                        >  2:2525008/1‑140 (MQ=255)
      gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGgttttttc                                                                                                                                      >  2:345922/1‑136 (MQ=255)
      gggTAACCCGGCTTGTTGCGCCAGTTCCATGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGc                                                                                                                                      >  1:674257/1‑140 (MQ=255)
           aCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGt                                                                                                                                 >  2:2392068/1‑140 (MQ=255)
              cGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTc                                                                                                                              <  1:1668300/140‑1 (MQ=255)
                 cTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTcgcg                                                                                                                           >  1:781467/1‑140 (MQ=255)
                 cTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTcgcg                                                                                                                           >  2:506271/1‑140 (MQ=255)
                    gttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGa                                                                                                                        <  2:1295313/140‑1 (MQ=255)
                          ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCATGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGt                                                                                                                  <  2:2486477/140‑1 (MQ=255)
                          ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCGTTTCGCGGGAATGGGt                                                                                                                  <  2:2059499/140‑1 (MQ=255)
                          ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGt                                                                                                                  <  1:2525008/140‑1 (MQ=255)
                          ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGt                                                                                                                  <  1:2550019/140‑1 (MQ=255)
                          ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGt                                                                                                                  <  1:1959883/140‑1 (MQ=255)
                          ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGt                                                                                                                  <  1:1736503/140‑1 (MQ=255)
                              ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGGATgggggggg                                                                                                              >  2:708266/1‑135 (MQ=255)
                              ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGATgggggggg                                                                                                              >  2:89678/1‑135 (MQ=255)
                              ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCGGGGATgggggggg                                                                                                              >  2:2013810/1‑135 (MQ=255)
                               tCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGAc                                                                                                             <  1:1175267/140‑1 (MQ=255)
                               tCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGATggggggggc                                                                                                             >  2:322143/1‑134 (MQ=255)
                                    gAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGa                                                                                                        <  2:1206389/140‑1 (MQ=255)
                                       tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAg                                                                                                     <  1:1641/140‑1 (MQ=255)
                                       tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAg                                                                                                     <  1:840838/140‑1 (MQ=255)
                                       tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAg                                                                                                     <  1:345922/140‑1 (MQ=255)
                                        gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGt                                                                                                    <  2:2274229/140‑1 (MQ=255)
                                        gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTCGCGGGGATGGGGGCGGCCATGACAgg                                                                                                    >  2:1740729/1‑139 (MQ=255)
                                        gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGGTTCGCGGGAATGGGTGGGACCCCGACaag                                                                                                    >  2:1217671/1‑138 (MQ=255)
                                        gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATGGGGGCGGCCCTGACAgg                                                                                                    >  2:1847635/1‑139 (MQ=255)
                                        gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTTGCGGGGATAGGTGGGGCCATGACAGt                                                                                                    >  1:2343336/1‑140 (MQ=255)
                                         ccACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGtc                                                                                                   >  1:334796/1‑140 (MQ=255)
                                          cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCGGGGAAGGGGGGGGCCATGACAGtct                                                                                                  >  1:1230029/1‑140 (MQ=255)
                                            cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTctct                                                                                                <  2:842462/140‑1 (MQ=255)
                                            cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGAGTTTCGCGGGAATGGGTGCGACCATGACAGTctct                                                                                                <  1:1659012/140‑1 (MQ=255)
                                            cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTTCGCGTTTCGCGGGGATGGGGGCGGCCATGACCGGctct                                                                                                >  2:2323004/1‑140 (MQ=255)
                                               tGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGATGGGGGGGGCCATGACAGTCTCtttt                                                                                             >  2:1340689/1‑140 (MQ=255)
                                                 ccATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTTCCGGGGATGGGGGGGACCATGACAGGCTCtttttt                                                                                           >  2:1016953/1‑140 (MQ=255)
                                                 acATTGGATTCGGCAAACCACTCCAGTAATTTCAGTCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGAGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGATGGGGGCGACCATGACAGTCTCtttttt                                                                                           >  2:2031012/2‑140 (MQ=255)
                                                      ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTa                                                                                      <  1:2350383/140‑1 (MQ=255)
                                                      ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTa                                                                                      <  1:210992/140‑1 (MQ=255)
                                                           cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCGTTTTGCGGGGATGGGGGCGACCCTGACAGTCTCTTTTTTTTTTATCGTg                                                                                 >  2:657818/1‑140 (MQ=255)
                                                           cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCCCGCTTCGCGGGGATGGGGGCGACCCTGGCAGTCTCTTTTTTTTTTTTCGTg                                                                                 >  2:2350383/1‑140 (MQ=255)
                                                           cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGATGGGGGGGACCAAGACAGGCTCTTTTTTTTGTATtgtg                                                                                 >  2:1456085/1‑140 (MQ=255)
                                                                 tCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTTCCCGTTTTGCGGGGATGGGGGCGACCACGACAGACTCTTTTTTTTTTATTGTGGGAAtc                                                                           >  2:450910/1‑140 (MQ=255)
                                                                    aCTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcatt                                                                        <  1:2031012/140‑1 (MQ=255)
                                                                      tCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTTTCGCGTTTTGCGGGGAGGGGGGCGGCCCTGGCAGTCTCTTTTTTCTGTATTGTGGGAAtcctttt                                                                      >  2:2541230/1‑140 (MQ=255)
                                                                      tCCAGTAATTACAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcatttt                                                                      <  1:450910/140‑1 (MQ=255)
                                                                        cAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAAtcattttca                                                                    <  1:1473683/140‑1 (MQ=255)
                                                                        cAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTCGCGGGGAAGGGGGGGGCCCTGACAGTCTCTTTTTTTTTTTTCGTGGGAAtcatttttt                                                                    >  2:1686256/1‑138 (MQ=255)
                                                                           tAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGTCCCGGTTCTCCGGGAAGGGGGCGGGCATTAAAGGCTTTTTTTTTTTTTTTGTTGGAAtcatttttattt                                                                 >  2:1024025/1‑140 (MQ=255)
                                                                            aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATcattttcatttt                                                                <  1:89678/140‑1 (MQ=255)
                                                                             aTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGGTTCGCGGGGAAGGGGGCGGCCCTGACAGGCTCTTTTTTTTTTATCGTGGGAATCatttttattttt                                                               >  2:1284/1‑140 (MQ=255)
                                                                                  aGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTTGCGGGGATGGGGGCGGCCCTGACAGTCTCTTTTTTTTTTTTTGTGGGAAACATTTTTAtttttttttt                                                          >  2:1434536/1‑138 (MQ=255)
                                                                                   ggCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGtt                                                         <  1:506271/140‑1 (MQ=255)
                                                                                    gCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATGGGGGCGGCCATGACAGTCTCTTTTTTTTTTATCGTGGGAATCATTTTTCTttttttttttt                                                        >  2:1917702/1‑139 (MQ=255)
                                                                                      cACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                                      <  1:768634/140‑1 (MQ=255)
                                                                                      cACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                                      <  1:657818/140‑1 (MQ=255)
                                                                                       aCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTCGCGGGGATGGGGGGGACCATGACAGTCTCTTTTTTTTTTATCGTGGAAATCATTTTCATTTTTATTGTTTGCt                                                     >  2:1642557/1‑140 (MQ=255)
                                                                                        cgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTTCGCGGGAATGGGGGCGACCATGACAGGCTCTTTTTTCTGTATCGTGGAAATCATTTTTATTTTTTTTTTTTGCTa                                                    >  1:1343396/1‑140 (MQ=255)
                                                                                        cgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGATTTCTCGCGCGCCTTGCGGGGGCGGGGGGGACCAAAAAAGAACCTTTTTTTTGTAACCGGGGAAACATTTTTATtttttttttttgtta                                                    >  2:1745125/1‑135 (MQ=255)
                                                                                          cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                  <  1:970832/140‑1 (MQ=255)
                                                                                          cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                  <  1:2198672/140‑1 (MQ=255)
                                                                                          cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCAGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                  <  2:2131880/140‑1 (MQ=255)
                                                                                            ttAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGc                                                <  1:1024025/140‑1 (MQ=255)
                                                                                              aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTTCCCCGTTTCGCGGGGAAGGGGGCGACCATGACAGTCTCTTTTTTCTGTTTCGTGGGAATCATTTTTATTTTTTTTTTTTGCTTATGGaa                                              >  2:1064221/1‑140 (MQ=255)
                                                                                              aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTCGCGGGGATGGGGGCGACCATGACAGGCTCTTTTTTTTGTATCGTGGGAATCATTTTTATTTTTTTTTTTAGCTAATGCaa                                              >  2:1401174/1‑140 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:1016953/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  2:334796/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:1340689/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:958345/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:1119655/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:495689/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:452003/140‑1 (MQ=255)
                                                                                               aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                             <  1:2040222/140‑1 (MQ=255)
                                                                                                    tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTa                                        <  1:322143/140‑1 (MQ=255)
                                                                                                    tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTa                                        <  2:781467/140‑1 (MQ=255)
                                                                                                     gAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGTTTCGCGGGAATGGGTGCGGCCATGACAGTCTCTTTTTTTTGTATCGTGGGAATCATTTTTATTTTTTTTTTTTGCTAATGCAATAGTTac                                       >  1:226285/1‑140 (MQ=255)
                                                                                                      aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCGGGGATGGGGGGGACCCTGACAGGCTCTTTTTTTTTTATCGTGGGAATCATTTTTATTTTTATTTTTAGCTAATGCAATAGTTact                                      >  2:499630/1‑140 (MQ=255)
                                                                                                      aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCGGGGATGGGGGCGACCCTGACAGGCTCTTTTTTTTTTATCGTGGGAATCATTTTTATTTTTTTTTTTTGCTAATGCAATAGTTact                                      >  2:1864588/1‑140 (MQ=255)
                                                                                                      aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGGCGCGTTTCCCGGGGAGGGGGGGGGCCCTGGCAGTCTCTTTTTTTTGTTTCCTGGGAATCCTTTTCATTTTTTTTGTTTGCTAATGCAATAATTact                                      >  2:1678149/1‑140 (MQ=255)
                                                                                                         cTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaa                                   <  1:1254367/140‑1 (MQ=255)
                                                                                                            tCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGGTTCGCGGGGATGGGGGGGGCCATGACAGTCTCTTTTTTTTTTTTCGTGGGAAACATTTTTATTTTTTTTTTTTGCTTATTCAATAGTTacttaactg                                >  2:644226/1‑140 (MQ=255)
                                                                                                                  cGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                          <  1:2242617/140‑1 (MQ=255)
                                                                                                                  cGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                          <  1:917433/140‑1 (MQ=255)
                                                                                                                  cGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                          <  1:1774706/140‑1 (MQ=255)
                                                                                                                  cGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                          <  2:1153961/140‑1 (MQ=255)
                                                                                                                   gCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGGATGGGGGCGGCCCTGACAGTCTCTTTTTT‑TTTTTCGTGGGAATCATTTTTATTTTTTTTTTTTGCTAATGCAATAGTTTCTGAACTGATCCGCTg                        >  2:1542096/1‑140 (MQ=255)
                                                                                                                    cTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATg                        <  1:1662149/140‑1 (MQ=255)
                                                                                                                      ggTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTCGCGGGGATGGGGGCGGCCATGACAGTCTCTTTTTTTTGTATCGTGGGAATCATTTTTATTTTTTTTTTTAGCTAATGCAATAGTTTCTGAACTGATCCGATGAg                      >  2:2251763/1‑140 (MQ=255)
                                                                                                                       gTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGt                     <  1:1064221/140‑1 (MQ=255)
                                                                                                                       gTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGt                     <  1:1745125/140‑1 (MQ=255)
                                                                                                                        tGCGGTGGCAACGGCGGGTTTTTTTCCGCGTTTCGCGGGGATGGGGGCGACCATGACAGTCTCTTTTTTTTGTATCGTGGGAATCATTTTTATTTTTTTTTTTTGCTAATGCAATAGTTACTGAACTGATCCGATgctgt                    >  2:450269/1‑136 (MQ=255)
                                                                                                                         gCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTa                   <  1:1155165/140‑1 (MQ=255)
                                                                                                                                  aCGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAAc          <  2:1571117/140‑1 (MQ=255)
                                                                                                                                  aCGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAAc          <  1:1003931/140‑1 (MQ=255)
                                                                                                                                  aCGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAAc          <  1:1989386/140‑1 (MQ=255)
                                                                                                                                         gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc   <  1:1847635/140‑1 (MQ=255)
                                                                                                                                         gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc   <  1:1864588/140‑1 (MQ=255)
                                                                                                                                         gTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc   <  1:2541230/140‑1 (MQ=255)
                                                                                                                                          ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCa  <  1:1434536/140‑1 (MQ=255)
                                                                                                                                          ttttCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCa  <  1:2164795/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCA  >  NC_000913/4223455‑4223732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑