Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,404,2150TG22.1% 455.2 / 54.6 181G244G (GGT→GGG) yhdHacrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (26/115);  new base G (40/0);  total (66/115)
Fisher's exact test for biased strand distribution p-value = 7.05e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 215 total aligned reads displayed.

GATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCT  >  NC_000913/3404078‑3404354
                                                                                                                                         |                                                                                                                                            
 atgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGggtggt                                                                                                                                           <  1:489180/140‑1 (MQ=255)
    aGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGgttt                                                                                                                                        >  1:1558974/1‑140 (MQ=255)
    aGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGgttt                                                                                                                                        >  1:803444/1‑140 (MQ=255)
      tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAc                                                                                                                                      <  2:2252422/140‑1 (MQ=255)
      tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGgttttc                                                                                                                                      >  1:1698288/1‑138 (MQ=255)
       ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct                                                                                                                                     <  2:768372/140‑1 (MQ=255)
        tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTActc                                                                                                                                    >  1:1213644/1‑140 (MQ=255)
        tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTActc                                                                                                                                    >  1:348752/1‑140 (MQ=255)
        tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTtctc                                                                                                                                    >  2:882949/1‑140 (MQ=255)
          ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  1:2513734/140‑1 (MQ=255)
           cGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGc                                                                                                                                 >  1:2359935/1‑140 (MQ=255)
              aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCTTGGCGGCCTGTGGGCTGGCCGGCGGTTTTAGTTAGCCaa                                                                                                                              >  2:516136/1‑140 (MQ=255)
                cccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAAcc                                                                                                                            >  1:1900350/1‑140 (MQ=255)
                 ccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCa                                                                                                                           <  2:592736/140‑1 (MQ=255)
                 ccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCa                                                                                                                           <  1:881794/140‑1 (MQ=255)
                   gTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  2:229082/131‑1 (MQ=255)
                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACgg                                                                                                                        >  1:3036199/1‑140 (MQ=255)
                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTTCCAACCACgg                                                                                                                        >  1:2978409/1‑140 (MQ=255)
                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCAccg                                                                                                                        >  2:714683/1‑138 (MQ=255)
                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCAACCACgg                                                                                                                        >  2:1171265/1‑140 (MQ=255)
                    tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTTCTCTGCCAACCAccg                                                                                                                        >  2:2844001/1‑138 (MQ=255)
                     ccTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGt                                                                                                                       <  2:1523895/140‑1 (MQ=255)
                     ccTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTTTCTGCCAAacacccg                                                                                                                       >  2:2119072/1‑137 (MQ=255)
                      ctctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTc                                                                                                                      <  2:2932088/140‑1 (MQ=255)
                       tctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCa                                                                                                                     <  1:155064/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:2160074/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  2:348752/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  2:19918/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  2:3015509/140‑1 (MQ=255)
                        ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  2:2578677/140‑1 (MQ=255)
                         tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGGGGGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTTTGCCAACCACCGTCCTg                                                                                                                   >  2:2890527/1‑140 (MQ=255)
                         tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTATTCTTCCCAacaccggcctg                                                                                                                   >  2:815331/1‑133 (MQ=255)
                              aaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGGCATGCCCtt                                                                                                              >  2:1204474/1‑140 (MQ=255)
                                 cAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTAt                                                                                                           <  2:684352/140‑1 (MQ=255)
                                    gTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCATGCCAtttttttt                                                                                                        >  2:1675493/1‑138 (MQ=255)
                                     tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCATGCCATTTATTTTg                                                                                                       >  2:1561318/1‑140 (MQ=255)
                                         ggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCCTTTATTCTGCGTa                                                                                                   >  2:2641803/1‑140 (MQ=255)
                                          gctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTaa                                                                                                  >  1:1392893/1‑140 (MQ=255)
                                           ctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAAt                                                                                                 >  1:908775/1‑140 (MQ=255)
                                            tgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATg                                                                                                <  2:2359935/140‑1 (MQ=255)
                                              gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGACGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑Tc                                                                                             >  1:2542417/1‑140 (MQ=255)
                                               ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑Tcc                                                                                            >  1:1499437/1‑140 (MQ=255)
                                                   aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGttt                                                                                        <  2:3044450/140‑1 (MQ=255)
                                                   aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCCACCACCGGCATGCCCTTTTTTTTGCGTAATGTTCCGtt                                                                                         >  2:1036216/1‑140 (MQ=255)
                                                    ttGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTg                                                                                       >  1:1735350/1‑140 (MQ=255)
                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGc                                                                                      <  1:2686513/140‑1 (MQ=255)
                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGc                                                                                      <  1:3086964/140‑1 (MQ=255)
                                                     tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGc                                                                                      <  1:1009140/140‑1 (MQ=255)
                                                     ggACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGc                                                                                      <  1:738868/139‑1 (MQ=255)
                                                      gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCa                                                                                     <  2:908775/140‑1 (MQ=255)
                                                      gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCa                                                                                     <  1:887815/140‑1 (MQ=255)
                                                       acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCaa                                                                                    <  1:1807041/140‑1 (MQ=255)
                                                       acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCaa                                                                                    <  1:1649151/140‑1 (MQ=255)
                                                       acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCaa                                                                                    <  2:1392893/140‑1 (MQ=255)
                                                       acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCaa                                                                                    <  2:1735350/140‑1 (MQ=255)
                                                         acCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGGCATGCCATTTATTTTGCGTAATG‑TCCGTTTGCaaag                                                                                  >  1:1994845/1‑138 (MQ=255)
                                                         acCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCCACCCCGGGCTTGCCATTTTTTTTGCGTAAAG‑TCCGTTTGCAAgg                                                                                  >  2:1100566/1‑140 (MQ=255)
                                                          ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAggg                                                                                 <  2:1464959/140‑1 (MQ=255)
                                                          ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAggg                                                                                 <  1:1171265/140‑1 (MQ=255)
                                                          ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTAT‑‑TGCGTAATG‑TCCGTTTGCAAGGGGt                                                                               <  1:2844001/140‑1 (MQ=255)
                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGt                                                                               <  1:2890527/140‑1 (MQ=255)
                                                            gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGt                                                                               <  1:1466640/140‑1 (MQ=255)
                                                             ttGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCATGCCCTTTTTTTTGCGGAATG‑TCCGTTTGCAAgggggg                                                                              >  2:484709/1‑138 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGa                                                                            <  2:18129/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGa                                                                            <  1:1137889/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGa                                                                            <  1:2265282/140‑1 (MQ=255)
                                                               ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGa                                                                            <  1:2641803/140‑1 (MQ=255)
                                                                gCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTTATTTGGCAAACCCGGGCATGCCCCTTTTTTTGCGTAATG‑TTCCTTTGGCAGgggggggt                                                                           >  2:2999158/1‑138 (MQ=255)
                                                                       aGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTaa                                                                    >  1:1865554/1‑140 (MQ=255)
                                                                          gCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTATTG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGa                                                                 <  1:516136/140‑1 (MQ=255)
                                                                          gCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGa                                                                 <  1:2496565/140‑1 (MQ=255)
                                                                          gCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCATGCCCTTTATTTTGCGTAATG‑TCCGTTTTCAAGGGGGGGGGTCAGGaactt                                                                 >  2:330949/1‑137 (MQ=255)
                                                                                 aaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGTCATGCCCTTTATTCTTCGGAATG‑TCCGTTTTCAAGGGGGGGATTCAGTAATGACGccacc                                                          >  2:427209/1‑140 (MQ=255)
                                                                                       cTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcg                                                    <  2:1865554/140‑1 (MQ=255)
                                                                                        tGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCATGCCCTTTATTTTGCGTAATG‑TCCGTTTGCAAGGGGGGGATTCAGTAATGACGCCACCCGAAagc                                                   >  2:2708843/1‑140 (MQ=255)
                                                                                              aaaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCCACCACGGGCCTGCCCTTTTTTTTGCGTAATG‑TCCGTTTGCAAGGGGGGGGTTTAGTAATGACGCCCCCAGAACGCCGCGca                                             >  2:2453819/1‑140 (MQ=255)
                                                                                               aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                            <  1:2206839/140‑1 (MQ=255)
                                                                                               aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                            <  1:1096655/140‑1 (MQ=255)
                                                                                                   aaTTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGc                                        <  1:677547/140‑1 (MQ=255)
                                                                                                       aCGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTgg                                    <  2:1017342/140‑1 (MQ=255)
                                                                                                          gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTTTTTTGCGTAATG‑TCCGTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAACGCCGCGCACAAACCCGGCAg                                 >  2:2035845/1‑140 (MQ=255)
                                                                                                             gcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAAACACCGTCATGCCCTTTATTTTGCGGAAAG‑TTCGTTTGTAAGGGGGGGGTTTAGTAATGACGCCACCAGAACGCCGCGCAACAGCCCCGCAGCGa                              >  2:327259/1‑140 (MQ=255)
                                                                                                              ccgCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCAATTTTTCTGCGGAATT‑TCCGTTTGCAAAGGGGGGAATTAGGAAAGACGCCACCAGAAAGCCGCGCACAAACCCCGCCGCGAc                             >  2:1104894/3‑140 (MQ=255)
                                                                                                                  gTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGt                         >  1:1765607/1‑140 (MQ=255)
                                                                                                                  gTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGt                         <  1:627139/140‑1 (MQ=255)
                                                                                                                     gcggcCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGc                      <  2:1765607/140‑1 (MQ=255)
                                                                                                                        gcCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGa                   >  1:1286218/1‑140 (MQ=255)
                                                                                                                             tgGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc              <  2:3111156/140‑1 (MQ=255)
                                                                                                                             tgGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc              >  1:447284/1‑140 (MQ=255)
                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:1029591/140‑1 (MQ=255)
                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:1950521/140‑1 (MQ=255)
                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:2315093/140‑1 (MQ=255)
                                                                                                                                  tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:327259/140‑1 (MQ=255)
                                                                                                                                   ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  2:2322336/140‑1 (MQ=255)
                                                                                                                                    gCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       <  2:1306976/140‑1 (MQ=255)
                                                                                                                                    gCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       <  2:1939801/140‑1 (MQ=255)
                                                                                                                                       ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        >  1:2102859/1‑136 (MQ=255)
                                                                                                                                         tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       >  1:2305949/1‑135 (MQ=255)
                                                                                                                                         tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       <  2:2305949/135‑1 (MQ=255)
                                                                                                                                         tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       <  2:2518155/135‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATG‑TCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCT  >  NC_000913/3404078‑3404354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑