Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,207	0	T	C	23.5%	181.1 / 23.9	85	S215G (AGT→GGT) ‡	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (59/6);  new base C (0/20);  total (59/26)
Fisher's exact test for biased strand distribution p-value = 1.64e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556068‑3556343                                                                                                                                             |                                                                                                                                         accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACt                                                                                                                                          >  1:1055806/1‑140 (MQ=255)  ccTCGCCCCTGCCGCGTTTTCCACCGACGAAAAAAAAGCGTTCGGTGATATTTTCACTTCCGCCTCCAAGCGAGACGGTATTCCCC‑GGCCCCTGGCCGCGCGGCAGGTTATAAATATTCTGTTCATGCAGGGAAAAACTc                                                                                                                                         <  2:946931/140‑1 (MQ=255)       gCACTGACGCGCTTTCCACCGACAAAAAAAAAGCGTTGGGTGATTTTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCC‑GGCCCCTGGTCGCGCGGCAGGTTATAAATATTTTGTTCATGCAGGAAAAACCCCCCCGc                                                                                                                                    <  2:2785204/140‑1 (MQ=255)        cACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                   >  2:461224/1‑140 (MQ=255)          cTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAg                                                                                                                                 >  1:1035936/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCTCCGCCAgtgt                                                                                                                              >  1:2658477/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                              >  1:3045474/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                              >  1:2535465/1‑140 (MQ=255)                  cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGa                                                                                                                         >  2:369809/1‑140 (MQ=255)                  cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGa                                                                                                                         >  1:578741/1‑140 (MQ=255)                  cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGa                                                                                                                         >  1:1408830/1‑140 (MQ=255)                         ccGACGAAAAAAAGGCGTTCGGTGTTTTTTTCCCTTTCGACTTCAAGCGAGACGTTTTTCCCC‑GGCCCCTGGTCGGGGGGCAGGTTATAAATTTTCTGTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:13775/140‑1 (MQ=255)                              gaaaaaaagGCGTTGGGTGATTTTTTCATTTTCGACTTCAGGCGGGACGGTATTCCCC‑GGCCCCGGGTGGCGCGGCAGTTTATGAATATTCTGTTCATGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAg                                                                                                             <  2:2535465/137‑1 (MQ=255)                              aaaaaaaaaGCGTTGGGTGATTTTTTCACTTTCGACTTCAAGCGAGACGGTATTCCCC‑GCCCCCTGGTCGCGCGGCAGGTTATGAATATTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAg                                                                                                             <  2:1790868/137‑1 (MQ=255)                               acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                            >  1:2882198/1‑140 (MQ=255)                                      ggCGTCCGGTAATTTTTTCACTTCCCTCTTCAGGCGGGACGTTTTACCCC‑GGCCCCTGGGCGCGCGGCAGGTTATAAATATCTTGTTAATGCAGGAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                     <  2:1055806/139‑1 (MQ=255)                                        cGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                   >  1:2493153/1‑140 (MQ=255)                                         gTTCGGTAATTTTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCC‑GGCCCCTGGTCGCGGGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcgc                                                                                                  <  2:2409865/140‑1 (MQ=255)                                          ttCGGTTATTTTTTCCTTTTCGACTTAAAGGGAGCCGGTATTCCCC‑GGCCCCTGGCCGGGGGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                 <  2:1879710/140‑1 (MQ=255)                                          ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                 >  2:2406511/1‑140 (MQ=255)                                          ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                 >  1:176558/1‑140 (MQ=255)                                           tCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                >  1:1386806/1‑140 (MQ=255)                                            cGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                               >  1:2409865/1‑140 (MQ=255)                                               tGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                            >  1:2398211/1‑140 (MQ=255)                                                tattttttCTCTTTCGACTTCAAGGGAGCCGGTTTTCCCC‑GGCCCCTGGTCGGGGGGCAGGTTATAAATTTTTTTTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCacac                                                                                           <  2:2882198/136‑1 (MQ=255)                                                tattttttCCTTTTCGACTTCAAGGGAGACGGTATTCCCC‑GCCCCCTGGTCGCGGGGCGGGTTATAAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCacac                                                                                           <  2:2778003/136‑1 (MQ=255)                                                  ttttttCCCTTTCAACTTAAAGGAAGACGAT‑TTACCCGGGCCCGTGGTCGCGCGGCAGGTTATGAATCTTTTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                         <  2:1406540/138‑1 (MQ=255)                                                         aCTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCTGCACACCAGCTAATa                                                                                  >  1:1398910/1‑140 (MQ=255)                                                         aCTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                  >  1:635682/1‑140 (MQ=255)                                                            ttCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                               >  2:712487/1‑140 (MQ=255)                                                             tCGACTTCAAGCGAGACGTTATTCCCC‑GGCCCCTGGCCGCGGGGCAGTTTATAAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                              <  2:2493153/140‑1 (MQ=255)                                                             tCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                              >  1:2735468/1‑140 (MQ=255)                                                             tCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                              >  1:511393/1‑140 (MQ=255)                                                             tCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                              >  1:788149/1‑140 (MQ=255)                                                               gACTTCAAGCGAGAGGGTTTTCCCC‑GGCCCCGGGGCGCGCGGCGGTTTATGAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt                                                                            <  2:1035936/140‑1 (MQ=255)                                                                    cAAGCGAGCCGGTATTCCCC‑GGCCCCGGGCCGCGGGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:578741/140‑1 (MQ=255)                                                                     aaGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCAc                                                                      >  1:2428489/1‑140 (MQ=255)                                                                       gagacggggTATTCCCC‑GGCCCCTGGTGGCGGGGCAGTTTATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                    <  2:2155003/133‑1 (MQ=255)                                                                           gaCGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                >  1:2427236/1‑140 (MQ=255)                                                                           gaCGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                >  2:1744021/1‑140 (MQ=255)                                                                            aCGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                               >  1:1467175/1‑140 (MQ=255)                                                                                     ccc‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                      >  1:221039/1‑140 (MQ=255)                                                                                         ggCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                   >  1:1417026/1‑140 (MQ=255)                                                                                          gCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAAtctgtctc                                                  >  1:2155003/1‑133 (MQ=255)                                                                                           ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  2:1585101/1‑140 (MQ=255)                                                                                           ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  2:1976669/1‑140 (MQ=255)                                                                                           ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:1670553/1‑140 (MQ=255)                                                                                           ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:1595855/1‑140 (MQ=255)                                                                                                     cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  2:997048/1‑140 (MQ=255)                                                                                                         gCAGTTTATGAATATTCTGTTCATGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                   <  2:917369/140‑1 (MQ=255)                                                                                                         gCAGGTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                   <  2:1946879/140‑1 (MQ=255)                                                                                                          gagtttATGAATTTTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:557521/136‑1 (MQ=255)                                                                                                          gagtttATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:1968048/136‑1 (MQ=255)                                                                                                          cAGTTTATAAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  1:369809/140‑1 (MQ=255)                                                                                                          cAGTTTATAAATATTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:2427236/140‑1 (MQ=255)                                                                                                          aagtttATAAATTTTCTTTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:635682/136‑1 (MQ=255)                                                                                                           gggTTATGAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCaa                                 <  2:176558/139‑1 (MQ=255)                                                                                                           aGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCaa                                 >  2:2727752/1‑140 (MQ=255)                                                                                                               tataAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                             <  2:221039/140‑1 (MQ=255)                                                                                                               tATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                             >  2:2479364/1‑140 (MQ=255)                                                                                                                  aaaTTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGCGTTa                          <  2:2071051/139‑1 (MQ=255)                                                                                                                       ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:19795/1‑140 (MQ=255)                                                                                                                       ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:2057500/1‑140 (MQ=255)                                                                                                                       ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:1401813/1‑140 (MQ=255)                                                                                                                       ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:861992/1‑140 (MQ=255)                                                                                                                       ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:595974/1‑140 (MQ=255)                                                                                                                       ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:1287596/1‑140 (MQ=255)                                                                                                                        tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  2:2186750/1‑140 (MQ=255)                                                                                                                        tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  2:726762/1‑140 (MQ=255)                                                                                                                        tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  1:1242766/1‑140 (MQ=255)                                                                                                                        tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  1:816636/1‑140 (MQ=255)                                                                                                                           gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                 >  2:1861813/1‑140 (MQ=255)                                                                                                                            ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  2:2318544/1‑140 (MQ=255)                                                                                                                            ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  1:2057500/140‑1 (MQ=255)                                                                                                                            ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  2:2582376/1‑140 (MQ=255)                                                                                                                            ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  1:3022247/1‑140 (MQ=255)                                                                                                                            ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  2:490221/1‑140 (MQ=255)                                                                                                                            ttCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  2:724190/140‑1 (MQ=255)                                                                                                                              cATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg              <  1:1287596/140‑1 (MQ=255)                                                                                                                              cATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGCGTTAAACGATGCCAcc              >  1:2071051/1‑139 (MQ=255)                                                                                                                                tGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg            <  1:2479364/140‑1 (MQ=255)                                                                                                                                 gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCTGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc           >  2:1570502/1‑140 (MQ=255)                                                                                                                                 gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc           >  1:669641/1‑140 (MQ=255)                                                                                                                                   aGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCga         <  2:1467175/140‑1 (MQ=255)                                                                                                                                    gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag        >  1:1613485/1‑140 (MQ=255)                                                                                                                                    gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag        >  1:2528737/1‑140 (MQ=255)                                                                                                                                    gggAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgag        >  2:318327/1‑140 (MQ=255)                                                                                                                                     gaaaaacCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCgaga       <  1:861992/138‑1 (MQ=255)                                                                                                                                      gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc      >  2:684507/1‑140 (MQ=255)                                                                                                                                      gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc      >  1:1253413/1‑140 (MQ=255)                                                                                                                                      gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc      >  1:2660521/1‑140 (MQ=255)                                                                                                                                        aaaaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt    <  1:1744021/140‑1 (MQ=255)                                                                                                                                          aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  2:511393/140‑1 (MQ=255)                                                                                                                                          aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  2:2735468/140‑1 (MQ=255)                                                                                                                                          aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  2:1613485/140‑1 (MQ=255)                                                                                                                                             tCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  2:959958/123‑1 (MQ=255)                                                                                                                                             tCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                >  1:959958/1‑123 (MQ=255)                                                                                                                                             |                                                                                                                                         ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556068‑3556343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑