BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,144,296	G→T	G344V (GGA→GTA)	dgcE →	putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,144,296	0	G	T	100.0%	570.2 / NA	162	G344V (GGA→GTA)	dgcE	putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (69/93); total (69/93)

Only 100 of 162 total aligned reads displayed.

GAAAGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGATGTTGT  >  NC_000913/2144160‑2144428

gAAAGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACt                                                                                                                                   <  2:390748/140‑1 (MQ=255)

gAAAGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACt                                                                                                                                   <  2:774660/140‑1 (MQ=255)

gAAAGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCACGTACt                                                                                                                                   <  1:546235/140‑1 (MQ=255)

  aaGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCa                                                                                                                                 >  2:1387691/1‑140 (MQ=255)

   aGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCAc                                                                                                                                <  1:2482767/140‑1 (MQ=255)

        aaCCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTc                                                                                                                           >  1:1246669/1‑140 (MQ=255)

        aaCCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTc                                                                                                                           >  1:874762/1‑140 (MQ=255)

             aCTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      <  1:764102/140‑1 (MQ=255)

             aCTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      <  2:1284685/140‑1 (MQ=255)

             aCTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      <  2:2653545/140‑1 (MQ=255)

             aCTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      <  1:1788198/140‑1 (MQ=255)

             aCTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      <  2:1709607/140‑1 (MQ=255)

                  cGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGAc                                                                                                                 <  1:2536587/140‑1 (MQ=255)

                  cGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGAc                                                                                                                 <  1:2381572/140‑1 (MQ=255)

                  cGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGAc                                                                                                                 <  1:2860667/140‑1 (MQ=255)

                  cGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGAc                                                                                                                 <  2:564725/140‑1 (MQ=255)

                     aaCGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGTGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTg                                                                                                              >  1:649818/1‑140 (MQ=255)

                     aaCGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTg                                                                                                              <  2:2073421/140‑1 (MQ=255)

                       cgcgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGc                                                                                                            <  1:2001032/140‑1 (MQ=255)

                         cgATGGAATATTCCGCTCTCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCg                                                                                                          >  1:535202/1‑140 (MQ=255)

                         cgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      >  1:1234833/1‑128 (MQ=255)

                         cgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCaa                                                                                                                      <  2:1234833/128‑1 (MQ=255)

                         cgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGAc                                                                                                                 <  1:2858176/133‑1 (MQ=255)

                         cgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGAc                                                                                                                 >  2:2858176/1‑133 (MQ=255)

                         cgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCg                                                                                                          >  1:2567509/1‑140 (MQ=255)

                         cgATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCg                                                                                                          >  1:1490299/1‑140 (MQ=255)

                            tGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCGCCTTTCAGCAACTGACCTGGCCggag                                                                                                       <  2:612768/140‑1 (MQ=255)

                            tGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCggag                                                                                                       <  2:1246669/140‑1 (MQ=255)

                               aaTATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGAt                                                                                                    <  1:2546617/140‑1 (MQ=255)

                                    tCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCt                                                                                                               >  1:109403/1‑124 (MQ=255)

                                    tCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCt                                                                                                               <  2:109403/124‑1 (MQ=255)

                                      cGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGc                                                                                                            <  1:690553/125‑1 (MQ=255)

                                      cGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGAt                                                                                                    <  1:1564358/133‑1 (MQ=255)

                                      cGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGAt                                                                                                    >  2:1564358/1‑133 (MQ=255)

                                      cGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATa                                                                                             <  1:116994/140‑1 (MQ=255)

                                       gCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATaa                                                                                            <  1:1646017/140‑1 (MQ=255)

                                        cTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGAt                                                                                                    <  1:2215371/131‑1 (MQ=255)

                                        cTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGAt                                                                                                    >  2:2215371/1‑131 (MQ=255)

                                        cTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATaaa                                                                                           >  1:206850/1‑140 (MQ=255)

                                        cTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATaaa                                                                                           >  1:828203/1‑140 (MQ=255)

                                           tCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGAt                                                                                        >  1:2417361/1‑140 (MQ=255)

                                           tCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGAt                                                                                        >  1:2985356/1‑140 (MQ=255)

                                             ggTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGAtct                                                                                      >  1:2156527/1‑140 (MQ=255)

                                                 tGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGAtctc                                                                                     <  2:2285716/137‑1 (MQ=255)

                                                 tGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGAtctc                                                                                     >  1:2285716/1‑137 (MQ=255)

                                                  ggCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAAc                                                                                 <  1:3109460/140‑1 (MQ=255)

                                                  ggCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAAc                                                                                 <  2:828203/140‑1 (MQ=255)

                                                     gTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAgg                                                                              >  1:2043360/1‑140 (MQ=255)

                                                       tAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGtt                                                                            >  2:2157598/1‑140 (MQ=255)

                                                         gTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGa                                                                          >  1:142168/1‑140 (MQ=255)

                                                          tGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaa                                                                         <  1:2656229/140‑1 (MQ=255)

                                                          tGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaa                                                                         <  1:1560033/140‑1 (MQ=255)

                                                           gggCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaaa                                                                        <  2:2417361/140‑1 (MQ=255)

                                                           gggCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaaa                                                                        <  2:2562569/140‑1 (MQ=255)

                                                             gCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAg                                                                      >  2:1841428/1‑140 (MQ=255)

                                                             gCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAg                                                                      <  2:3139410/140‑1 (MQ=255)

                                                              cACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGc                                                                     >  1:654804/1‑140 (MQ=255)

                                                              cACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGc                                                                     >  2:1120871/1‑140 (MQ=255)

                                                              cACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGc                                                                     >  2:2438279/1‑140 (MQ=255)

                                                               aCCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaa                                                                         <  1:2325398/135‑1 (MQ=255)

                                                                 cGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGa                                                                  >  1:152732/1‑140 (MQ=255)

                                                                   aGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaa                                                                         <  2:2697143/131‑1 (MQ=255)

                                                                   aGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGaa                                                                         >  1:2697143/1‑131 (MQ=255)

                                                                   aGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATa                                                                >  1:1289653/1‑140 (MQ=255)

                                                                   aGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATa                                                                >  1:3024627/1‑140 (MQ=255)

                                                                   aGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGG‑‑GGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAg                                                              <  1:1540947/140‑1 (MQ=255)

                                                                     ggACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAg                                                              <  2:2326002/140‑1 (MQ=255)

                                                                     ggACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAg                                                              <  2:142168/140‑1 (MQ=255)

                                                                       aCAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCg                                                            <  1:1164326/140‑1 (MQ=255)

                                                                         aaTGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGt                                                          <  1:1120871/140‑1 (MQ=255)

                                                                         aaTGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGt                                                          <  2:2043360/140‑1 (MQ=255)

                                                                         aaTGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGt                                                          <  2:1289653/140‑1 (MQ=255)

                                                                          aTGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTg                                                         <  2:1316766/140‑1 (MQ=255)

                                                                           tGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGa                                                        >  1:484491/1‑140 (MQ=255)

                                                                              cTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAGCTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTg                                                         <  2:711394/136‑1 (MQ=255)

                                                                               tGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATa                                                    <  2:649818/140‑1 (MQ=255)

                                                                               tGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATa                                                    <  2:1533890/140‑1 (MQ=255)

                                                                               tGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATa                                                    <  1:2157598/140‑1 (MQ=255)

                                                                                 cAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGt                                                          <  2:2910056/132‑1 (MQ=255)

                                                                                 cAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATaaa                                                  <  2:284984/140‑1 (MQ=255)

                                                                                 cAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATa                                                    >  2:202272/1‑138 (MQ=255)

                                                                                 cAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATa                                                    <  1:202272/138‑1 (MQ=255)

                                                                                        acaaAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAac                                                 <  2:2009520/134‑1 (MQ=255)

                                                                                        acaaAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAac                                                 >  1:2009520/1‑134 (MQ=255)

                                                                                         caaAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTAtt                                          <  1:822329/140‑1 (MQ=255)

                                                                                            aGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCaa                                       >  2:2053814/1‑140 (MQ=255)

                                                                                              cgcTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATg                                     >  1:1657583/1‑140 (MQ=255)

                                                                                                ctctGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGa                                   >  1:759875/1‑140 (MQ=255)

                                                                                                ctctGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGa                                   >  1:1303028/1‑140 (MQ=255)

                                                                                                  ctGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGaaa                                 <  2:3024627/140‑1 (MQ=255)

                                                                                                   tGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGaaaa                                >  2:1228498/1‑140 (MQ=255)

                                                                                                   tGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGaaaa                                >  2:1871992/1‑140 (MQ=255)

                                                                                                    gCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGaaaaa                               <  1:275468/140‑1 (MQ=255)

                                                                                                     ccAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAAc                              <  1:2625856/140‑1 (MQ=255)

                                                                                                       aGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGc                            <  2:2247529/140‑1 (MQ=255)

                                                                                                               gggTACAGTCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACaa                    >  1:2683997/1‑140 (MQ=255)

                                                                                                                       tCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATg            <  2:152732/140‑1 (MQ=255)

                                                                                                                       tCAGGAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATg            <  1:1228498/140‑1 (MQ=255)

                                                                                                                           gAAGAGCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGa        <  2:344220/140‑1 (MQ=255)

                                                                                                                              gagCTGCGCGTACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGAtgt     >  2:446819/1‑140 (MQ=255)

GAAAGCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGATGTTGT  >  NC_000913/2144160‑2144428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`