Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,473,281	C→A	intergenic (‑35/‑323)	yfdO ← / → yfdP	CPS‑53 (KpLE1) prophage; putative defective phage replication protein O/CPS‑53 (KpLE1) prophage; protein YfdP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,473,281	0	C	A	100.0%	346.2 / NA	98	intergenic (‑35/‑323)	yfdO/yfdP	CPS‑53 (KpLE1) prophage; putative defective phage replication protein O/CPS‑53 (KpLE1) prophage; protein YfdP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/49);  total (49/49)

TCCAGTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAG  >  NC_000913/2473147‑2473416                                                                                                                                       |                                                                                                                                        tCCAGTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAAt                                                                                                                                    >  1:59468/1‑140 (MQ=255) tCCAGTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAAt                                                                                                                                    >  2:1668291/1‑140 (MQ=255)   cAGTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATAc                                                                                                                                  <  1:877777/140‑1 (MQ=255)   cAGTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATAc                                                                                                                                  <  1:2418431/140‑1 (MQ=255)     gTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGC‑TTTTAGGGATGAATACAAc                                                                                                                               <  2:22098/140‑1 (MQ=255)      ttCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAAc                                                                                                                               <  1:1639280/140‑1 (MQ=255)      ttCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAAc                                                                                                                               >  1:419797/1‑140 (MQ=255)      ttCCGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAAc                                                                                                                               <  2:305145/140‑1 (MQ=255)       tCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAAcc                                                                                                                              >  1:2203932/1‑140 (MQ=255)       tCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAAcc                                                                                                                              >  2:1686056/1‑140 (MQ=255)         ggTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGc                                                                                                                            <  1:63020/140‑1 (MQ=255)           ttCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATAc                                                                                                                                  >  1:420366/1‑132 (MQ=255)           ttCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATAc                                                                                                                                  <  2:420366/132‑1 (MQ=255)           ttCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACaa                                                                                                                                >  1:2580697/1‑134 (MQ=255)           ttCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACaa                                                                                                                                <  2:2580697/134‑1 (MQ=255)           ttCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCAt                                                                                                                          >  1:600608/1‑140 (MQ=255)                    aCGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGc                                                                                                                 <  2:59468/140‑1 (MQ=255)                       aaCCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAg                                                                                                              <  1:537393/140‑1 (MQ=255)                         ccaccTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGtt                                                                                                            <  1:3470688/140‑1 (MQ=255)                                  cGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATcc                                                                                                   >  1:1893208/1‑140 (MQ=255)                                   gTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCt                                                                                                  <  1:396063/140‑1 (MQ=255)                                    tCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATg                                                                                                                   <  1:1594827/122‑1 (MQ=255)                                    tCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATg                                                                                                                   >  2:1594827/1‑122 (MQ=255)                                       caTTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTc                                                                                              >  1:3224299/1‑140 (MQ=255)                                       caTTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTc                                                                                              >  1:2434801/1‑140 (MQ=255)                                            ggcggcCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCg                                                                                         >  2:691511/1‑140 (MQ=255)                                             gcggcCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGt                                                                                        >  2:1336890/1‑140 (MQ=255)                                              cggcCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTc                                                                                       >  1:967002/1‑140 (MQ=255)                                                gcCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTg                                                                                     >  1:3378973/1‑140 (MQ=255)                                                 ccTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGc                                                                                    >  1:943016/1‑140 (MQ=255)                                                 ccTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGc                                                                                    <  1:2881085/140‑1 (MQ=255)                                                    gCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATCCAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCgtg                                                                                 <  1:2817516/140‑1 (MQ=255)                                                    gCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCgtg                                                                                 <  2:2434801/140‑1 (MQ=255)                                                     cTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCt                                                                                                  >  2:1050057/1‑122 (MQ=255)                                                     cTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCt                                                                                                  <  1:1050057/122‑1 (MQ=255)                                                      tCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTc                                                                               <  2:600608/140‑1 (MQ=255)                                                      tCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCCTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTc                                                                               <  2:2597832/140‑1 (MQ=255)                                                              ggCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       <  1:2568430/140‑1 (MQ=255)                                                              ggCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       <  2:2891914/140‑1 (MQ=255)                                                              ggCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       >  1:965676/1‑140 (MQ=255)                                                              ggCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       >  1:938582/1‑140 (MQ=255)                                                               gCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       >  2:1632110/1‑139 (MQ=255)                                                               gCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       <  1:1632110/139‑1 (MQ=255)                                                               gCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGa                                                                      >  2:1903108/1‑140 (MQ=255)                                                               gCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGa                                                                      >  2:1883581/1‑140 (MQ=255)                                                               gCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGa                                                                      >  1:1408638/1‑140 (MQ=255)                                                               gCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGa                                                                      >  2:3078116/1‑140 (MQ=255)                                                                ggatgatTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGaa                                                                     <  1:755573/139‑1 (MQ=255)                                                                cGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGaa                                                                     <  2:943016/140‑1 (MQ=255)                                                                cGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGaa                                                                     >  2:3445713/1‑140 (MQ=255)                                                                  atgatTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       <  2:230491/136‑1 (MQ=255)                                                                  atgatTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCg                                                                       >  1:230491/1‑136 (MQ=255)                                                                   tgatTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCg                                                                  >  1:484055/1‑140 (MQ=255)                                                                      tttttttCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTcc                                                               <  2:2203932/140‑1 (MQ=255)                                                                             ccAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACgag                                                        <  1:1336890/140‑1 (MQ=255)                                                                               agagCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGaa                                                      <  1:1895944/140‑1 (MQ=255)                                                                               agagCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGaa                                                      <  1:1686056/140‑1 (MQ=255)                                                                               agagCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGaa                                                      <  2:1408638/140‑1 (MQ=255)                                                                                 agCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAgg                                                    >  1:301750/1‑140 (MQ=255)                                                                                         tgCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTAt                                            <  1:3445713/140‑1 (MQ=255)                                                                                           cgcAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCt                                          <  1:1799423/140‑1 (MQ=255)                                                                                           cgcAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCt                                          >  1:3556569/1‑140 (MQ=255)                                                                                             cAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGa                                        >  1:421644/1‑140 (MQ=255)                                                                                             cAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGa                                        >  1:528363/1‑140 (MQ=255)                                                                                             cAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGa                                        <  2:1554415/140‑1 (MQ=255)                                                                                             cAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGa                                        >  1:1554415/1‑140 (MQ=255)                                                                                              aGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGAc                                       <  1:1091840/140‑1 (MQ=255)                                                                                              aGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTCCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGAc                                       >  1:1725387/1‑140 (MQ=255)                                                                                               ggTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACg                                      >  1:444133/1‑140 (MQ=255)                                                                                                gTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACgg                                     >  1:1415608/1‑140 (MQ=255)                                                                                                 tCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCt                                          <  1:732785/134‑1 (MQ=255)                                                                                                 tCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCt                                          >  2:732785/1‑134 (MQ=255)                                                                                                   aaaTCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATc                                  >  1:1404214/1‑140 (MQ=255)                                                                                                   aaaTCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATc                                  <  2:484055/140‑1 (MQ=255)                                                                                                   aaaTCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATc                                  <  2:967002/140‑1 (MQ=255)                                                                                                   aaaTCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATc                                  >  1:2331755/1‑140 (MQ=255)                                                                                                   aaaTCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATc                                  <  2:1893208/140‑1 (MQ=255)                                                                                                     atcatcCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAACGTCCGGACGAGAAGGCTTTTTATCTGAGGGATCag                                >  2:1832622/1‑139 (MQ=255)                                                                                                       catcCTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGt                              <  2:1415608/140‑1 (MQ=255)                                                                                                          ccTGCGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGtttt                           >  2:2939928/1‑140 (MQ=255)                                                                                                              cGTTCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGAtt                       >  2:1283772/1‑140 (MQ=255)                                                                                                                 tCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTa                    <  2:2710631/140‑1 (MQ=255)                                                                                                                 tCCCCACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTa                    >  2:182359/1‑140 (MQ=255)                                                                                                                   cccACTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACt                  >  2:2527298/1‑140 (MQ=255)                                                                                                                      aCTGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGAc               >  1:1034377/1‑140 (MQ=255)                                                                                                                        tGGCGCTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACgg             >  1:159539/1‑140 (MQ=255)                                                                                                                          gcgcTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGAt           >  2:1266179/1‑140 (MQ=255)                                                                                                                           cgcTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATc          >  1:2966075/1‑140 (MQ=255)                                                                                                                            gcTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATcc         <  1:3370438/140‑1 (MQ=255)                                                                                                                            gcTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATcc         <  1:1668291/140‑1 (MQ=255)                                                                                                                            gcTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATcc         <  2:3224299/140‑1 (MQ=255)                                                                                                                            gcTTTTTAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATcc         <  1:1266179/140‑1 (MQ=255)                                                                                                                              cttttAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATcccc       >  1:1416381/2‑140 (MQ=255)                                                                                                                                tttAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGc     <  2:1416381/140‑1 (MQ=255)                                                                                                                                 ttAGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGcc    <  2:444133/140‑1 (MQ=255)                                                                                                                                   aGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAg  <  1:1883581/140‑1 (MQ=255)                                                                                                                                   aGGGATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAg  <  1:3078116/140‑1 (MQ=255)                                                                                                                                    gggATGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATcc         <  1:1105321/132‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TCCAGTTCGGTTCTTTGGGACGAACCACCTCGCCGTCACATTCGGCGGCCTGCTCGTACAGGGCGATGATTTTTTTCCAGAGCCACTGTGCGCAGGTCAAATCATCCTGCGTTCCCCACTGGCGCTTTTTAGGGCTGAATACAACCGCATCAGGATGGCGAGTTAAAAAATCCTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAG  >  NC_000913/2473147‑2473416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑