Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 552,943 C→A S53I (AGC→ATC)  purE ← N(5)‑carboxyaminoimidazole ribonucleotide mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913552,9430CA100.0% 594.1 / NA 164S53I (AGC→ATC) purEN(5)‑carboxyaminoimidazole ribonucleotide mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (79/85);  total (79/85)

Only 100 of 164 total aligned reads displayed.

CAGTGCGGCGCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCG  >  NC_000913/552807‑553078
                                                                                                                                        |                                                                                                                                       
cAGTGCGGCGCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGa                                                                                                                                      <  1:936006/140‑1 (MQ=255)
   tGCGGCGCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACa                                                                                                                                   >  1:2640514/1‑140 (MQ=255)
   tGCGGCGCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACa                                                                                                                                   >  1:1451835/1‑140 (MQ=255)
   tGCGGCGCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACa                                                                                                                                   >  1:800356/1‑140 (MQ=255)
    gcggcgCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAg                                                                                                                                  <  2:1195772/140‑1 (MQ=255)
    gcggcgCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAg                                                                                                                                  <  2:379905/140‑1 (MQ=255)
        cgcTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTa                                                                                                                              >  1:3531809/1‑140 (MQ=255)
        cgcTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTa                                                                                                                              >  1:410564/1‑140 (MQ=255)
         gcTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTAt                                                                                                                             <  2:410564/140‑1 (MQ=255)
         gcTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTAt                                                                                                                             <  1:160113/140‑1 (MQ=255)
            ctGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCgg                                                                                                                          <  2:2828654/140‑1 (MQ=255)
             tGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCggg                                                                                                                         >  1:40189/1‑140 (MQ=255)
             tGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCggg                                                                                                                         >  2:1440872/1‑140 (MQ=255)
             tGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCggg                                                                                                                         >  1:327291/1‑140 (MQ=255)
             tGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCggg                                                                                                                         >  1:341806/1‑140 (MQ=255)
                    gCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCgg                                                                                                                  >  2:2938119/1‑140 (MQ=255)
                    gCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCgg                                                                                                                  <  2:3460102/140‑1 (MQ=255)
                    gCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCgg                                                                                                                  >  2:3498632/1‑140 (MQ=255)
                     cACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGt                                                                                                                 <  2:3299231/140‑1 (MQ=255)
                     cACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGt                                                                                                                 <  2:836028/140‑1 (MQ=255)
                     cACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGt                                                                                                                 <  1:1114667/140‑1 (MQ=255)
                     cACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGt                                                                                                                 <  1:2600828/140‑1 (MQ=255)
                     cACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGt                                                                                                                 <  2:1310513/140‑1 (MQ=255)
                       cGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGa                                                                                                               >  2:2165964/1‑140 (MQ=255)
                         cccAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGt                                                                                                                 <  2:883624/136‑1 (MQ=255)
                         cccAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGc                                                                                                             >  2:2362433/1‑140 (MQ=255)
                         cccAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGc                                                                                                             >  2:1785902/1‑140 (MQ=255)
                          ccAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCa                                                                                                            <  2:1865859/140‑1 (MQ=255)
                            aGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGa                                                                                                          <  1:2264275/140‑1 (MQ=255)
                              cACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCa                                                                                                            <  1:2775839/136‑1 (MQ=255)
                              cACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGaaa                                                                                                        <  1:3498632/140‑1 (MQ=255)
                                   gCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACt                                                                                                   >  1:1579541/1‑140 (MQ=255)
                                    cACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACtt                                                                                                  >  1:3209620/1‑140 (MQ=255)
                                    cACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACtt                                                                                                  >  1:3556974/1‑140 (MQ=255)
                                        aGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATC‑GGGGTGCGGTGAGCAGAAACCACTTCAACg                                                                                             <  2:1678713/140‑1 (MQ=255)
                                         gCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACg                                                                                             <  2:2321929/140‑1 (MQ=255)
                                         gCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACg                                                                                             <  2:327291/140‑1 (MQ=255)
                                         gCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACg                                                                                             <  1:1057032/140‑1 (MQ=255)
                                                ggcggcAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCg                                                                                      <  2:1041952/140‑1 (MQ=255)
                                                ggcggcAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCg                                                                                      <  2:800356/140‑1 (MQ=255)
                                                  cggcAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCggg                                                                                    <  2:937891/140‑1 (MQ=255)
                                                  cggcAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCggg                                                                                    <  1:878754/140‑1 (MQ=255)
                                                                  cAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGa                                                                    >  2:182061/1‑140 (MQ=255)
                                                                      tgcgctgcgcCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGAt                                                                >  1:384138/1‑140 (MQ=255)
                                                                       gcgctgcgcCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGAtt                                                               >  2:686899/1‑140 (MQ=255)
                                                                             cgccgcCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTcggcg                                                         <  1:3503556/140‑1 (MQ=255)
                                                                             cgccgcCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTcggcg                                                         <  2:2564073/140‑1 (MQ=255)
                                                                             cgccgcCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTcggcg                                                         <  1:2338792/140‑1 (MQ=255)
                                                                             cgccgcCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTcggcg                                                         <  2:1023106/140‑1 (MQ=255)
                                                                                cgccTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGgcggcg                                                      <  2:3242076/140‑1 (MQ=255)
                                                                                cgccTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGgcggcg                                                      <  2:3565432/140‑1 (MQ=255)
                                                                                cgccTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGgcggcg                                                      <  1:1417570/140‑1 (MQ=255)
                                                                                cgccTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGgcggcg                                                      <  2:204257/140‑1 (MQ=255)
                                                                                  ccTGCGCCCGCAATAATCACCTGGTAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGaa                                                    >  1:1364736/1‑140 (MQ=255)
                                                                                    tGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTCCGGCGGCGAACt                                                  <  1:1785902/140‑1 (MQ=255)
                                                                                          cGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATgg                                            <  2:703576/140‑1 (MQ=255)
                                                                                                 atCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCa                                     >  1:2922885/1‑140 (MQ=255)
                                                                                                    aCCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTc                                  <  1:64921/140‑1 (MQ=255)
                                                                                                       tGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCt                               >  1:94511/1‑140 (MQ=255)
                                                                                                       tGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCt                               >  1:3314306/1‑140 (MQ=255)
                                                                                                         aTAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGt                                   >  1:3489878/1‑134 (MQ=255)
                                                                                                         aTAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGt                                   <  2:3489878/134‑1 (MQ=255)
                                                                                                         aTAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCttt                             >  1:2391576/1‑140 (MQ=255)
                                                                                                         aTAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCttt                             >  1:1234313/1‑140 (MQ=255)
                                                                                                         aTAACCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCttt                             >  1:2080324/1‑140 (MQ=255)
                                                                                                           aaCCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTg                           >  2:3121621/1‑140 (MQ=255)
                                                                                                           aaCCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTg                           <  1:686899/140‑1 (MQ=255)
                                                                                                           aaCCGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTg                           <  1:2238570/140‑1 (MQ=255)
                                                                                                             ccGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGa                         <  1:182061/140‑1 (MQ=255)
                                                                                                             ccGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGa                         <  2:1540891/140‑1 (MQ=255)
                                                                                                             ccGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACCGCATGGTAGCCCAGTCGCTTTTGGa                         <  2:3209620/140‑1 (MQ=255)
                                                                                                              cGTTCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAc                        <  2:1579541/140‑1 (MQ=255)
                                                                                                                ttCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAccc                      >  2:345284/1‑140 (MQ=255)
                                                                                                                ttCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAccc                      >  1:2401033/1‑140 (MQ=255)
                                                                                                                ttCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAccc                      >  1:1512136/1‑140 (MQ=255)
                                                                                                                ttCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAccc                      >  1:1995315/1‑140 (MQ=255)
                                                                                                                ttCTCTTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGCGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAccc                      >  2:2848589/1‑140 (MQ=255)
                                                                                                                   tctTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAc                        <  1:2582041/135‑1 (MQ=255)
                                                                                                                   tctTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGAc                        >  2:2582041/1‑135 (MQ=255)
                                                                                                                   tctTCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCAt                   <  1:845476/140‑1 (MQ=255)
                                                                                                                     ttCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGt                                   >  2:1088738/1‑122 (MQ=255)
                                                                                                                     ttCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGa                         >  2:3532149/1‑132 (MQ=255)
                                                                                                                     ttCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCa                 >  2:1599238/1‑140 (MQ=255)
                                                                                                                     ttCGGCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCa                 >  1:2150022/1‑140 (MQ=255)
                                                                                                                        ggCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGa              >  1:1046824/1‑140 (MQ=255)
                                                                                                                        ggCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGa              >  1:1420368/1‑140 (MQ=255)
                                                                                                                        ggCGCTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGa              >  1:2971894/1‑140 (MQ=255)
                                                                                                                          cgcTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATg            >  2:3419222/1‑140 (MQ=255)
                                                                                                                          cgcTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATg            >  2:28705/1‑140 (MQ=255)
                                                                                                                          cgcTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATg            >  2:2073913/1‑140 (MQ=255)
                                                                                                                            cTTTCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGc          >  1:2024689/1‑140 (MQ=255)
                                                                                                                               tCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGac       >  2:3328505/1‑140 (MQ=255)
                                                                                                                               tCGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGac       >  2:1626502/1‑140 (MQ=255)
                                                                                                                                cGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGaca      <  1:1626502/140‑1 (MQ=255)
                                                                                                                                cGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGaca      <  2:1364736/140‑1 (MQ=255)
                                                                                                                                cGGCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGaca      <  1:3328505/140‑1 (MQ=255)
                                                                                                                                  gCGAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACAcg    <  1:345284/140‑1 (MQ=255)
                                                                                                                                    gAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGTGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACAcgcg  <  2:2378/140‑1 (MQ=255)
                                                                                                                                    gAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACAcgcg  <  2:270576/140‑1 (MQ=255)
                                                                                                                                    gAAGATGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACAcgcg  <  1:1116277/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CAGTGCGGCGCTCTGTACTGGCACGCCCAGCACCGGCACCAGCGTTTTGGCGGCAATCATGCCTGGCAGATGCGCTGCGCCGCCTGCGCCCGCAATAATCACCTGATAACCGTTCTCTTCGGCGCTTTCGGCGAAGCTGAACAGTTTATCGGGGGTGCGGTGAGCAGAAACCACTTCAACGTGGTGCGGGACATTCAGGATTTCGAAGATTTCGGCGGCGAACTGCATGGTAGCCCAGTCGCTTTTGGACCCCATCACGATGGCGACACGCG  >  NC_000913/552807‑553078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑