Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,427	C→A	G209G (GGC→GGA)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427	0	C	A	100.0%	129.2 / NA	38	G209G (GGC→GGA)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/18);  total (20/18)

CAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGC  >  NC_000913/3290‑3561                                                                                                                                          |                                                                                                                                       cAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGAtt                                                                                                                                      >  2:715995/1‑140 (MQ=255)     tGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGAttcatt                                                                                                                                  <  2:263687/140‑1 (MQ=255)       ccAGGGTTTGATGAGTGGCTGTGGGCGCTGGCGTAGCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGCATCGCCGCCAGGATTGCATTGCCCACGGGCGACAACTGGCAGGattcattcc                                                                                                                                >  2:195841/1‑139 (MQ=255)             tttGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTg                                                                                                                          <  1:652879/140‑1 (MQ=255)             tttGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCCGGCAGGATTCATTCACGCCTg                                                                                                                          <  2:719078/140‑1 (MQ=255)                            tgGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGcc                                                                                                           >  2:664754/1‑140 (MQ=255)                                  cTGGCGTATCCGGGGATTAAAGTCTCGATGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCt                                                                                                     >  1:715733/1‑140 (MQ=255)                                  cTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGcc                                                                                                           >  1:149084/1‑134 (MQ=255)                                  cTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGcc                                                                                                           <  2:149084/134‑1 (MQ=255)                                       gTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcg                                                                                                <  1:715995/140‑1 (MQ=255)                                         aTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcgcg                                                                                              <  1:601330/140‑1 (MQ=255)                                         aTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcgcg                                                                                              <  1:545644/140‑1 (MQ=255)                                         aTCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCcgcg                                                                                              >  2:824626/1‑140 (MQ=255)                                              gggATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCt                                                                                         <  1:664754/140‑1 (MQ=255)                                                    aaaGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGaa                                                                                   >  2:823742/1‑140 (MQ=255)                                                            gACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTa                                                                           >  2:202083/1‑140 (MQ=255)                                                               ggCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCg                                                                        <  2:715733/140‑1 (MQ=255)                                                                 cAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCt                                                                      <  1:489233/140‑1 (MQ=255)                                                                 cAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCt                                                                      <  2:859659/140‑1 (MQ=255)                                                                    aaGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGaa                                                                   <  1:823742/140‑1 (MQ=255)                                                                      gCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAAcc                                                                 <  1:301055/140‑1 (MQ=255)                                                                           ggCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGaa                                                                   <  1:911992/133‑1 (MQ=255)                                                                           ggCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGaa                                                                   >  2:911992/1‑133 (MQ=255)                                                                              tATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTg                                                         <  1:202083/140‑1 (MQ=255)                                                                                         cgcAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTAc                                                >  2:662836/1‑138 (MQ=255)                                                                                         cgcAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTAc                                                <  1:662836/138‑1 (MQ=255)                                                                                                        aGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCcggcag                               >  1:787002/1‑140 (MQ=255)                                                                                                        aGGATTGCATTGCGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCcggcag                               >  2:854398/1‑140 (MQ=255)                                                                                                           attgcattgcGCACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTGTCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCcggcaggcg                            <  1:854398/140‑1 (MQ=255)                                                                                                                    cgcACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCcggcaggcgcggcaggcg                   >  2:443693/1‑140 (MQ=255)                                                                                                                    cgcACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCcggcaggcgcggcaggcg                   >  1:284865/1‑140 (MQ=255)                                                                                                                     gcACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCgg                  >  2:77074/1‑140 (MQ=255)                                                                                                                      cACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGt                 >  2:397108/1‑140 (MQ=255)                                                                                                                      cACGGGCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGt                 >  1:649969/1‑140 (MQ=255)                                                                                                                         gggCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTcgc              >  1:364834/1‑140 (MQ=255)                                                                                                                          ggCGACATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTcgcg             <  1:824626/140‑1 (MQ=255)                                                                                                                              aCATCTGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCATGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGgaa         >  2:949858/1‑140 (MQ=255)                                                                                                                                   tGGCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGgc    >  1:496043/1‑140 (MQ=255)                                                                                                                                     gCAGGATTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGgcgc  >  2:526908/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CAAGTGCCAGGGTTTGATGAGTGGCTGTGGGTGCTGGCGTATCCGGGGATTAAAGTCTCGACGGCAGAAGCCAGGGCTATTTTACCGGCGCAGTATCGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGC  >  NC_000913/3290‑3561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑