Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,052,893	G→T	P209Q (CCG→CAG)	ubiH ←	2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,052,893	0	G	T	100.0%	186.2 / NA	53	P209Q (CCG→CAG)	ubiH	2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/31);  total (22/31)

CAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGG  >  NC_000913/3052756‑3053027                                                                                                                                          |                                                                                                                                       cAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCtgg                                                                                                                                      <  2:925888/140‑1 (MQ=255)   aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGcc                                                                                                                                    <  2:931162/140‑1 (MQ=255)   aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGcc                                                                                                                                    <  1:447210/140‑1 (MQ=255)     tCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCAt                                                                                                                                  <  1:189494/140‑1 (MQ=255)      cGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATg                                                                                                                                 >  2:110926/1‑140 (MQ=255)               aaGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTaaa                                                                                                                        <  1:890658/140‑1 (MQ=255)                aGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAg                                                                                                                       >  2:949826/1‑140 (MQ=255)                         ggAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAACGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCaaaa                                                                                                              <  1:845284/140‑1 (MQ=255)                            gTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAgcg                                                                                                           <  2:532956/140‑1 (MQ=255)                             ttCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAgcgc                                                                                                          <  1:110926/140‑1 (MQ=255)                              tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAgcgcg                                                                                                         <  1:214203/140‑1 (MQ=255)                               cACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAgcgcgc                                                                                                        <  1:510517/140‑1 (MQ=255)                                           tctcGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                            <  2:754804/140‑1 (MQ=255)                                           tctcGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCa                                                                                            <  1:710090/140‑1 (MQ=255)                                                    tCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTa                                                                                   >  2:362777/1‑140 (MQ=255)                                                       aCGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCGTGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCa                                                                                >  1:242980/1‑140 (MQ=255)                                                            aacaCCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGtt                                                                           <  2:207357/140‑1 (MQ=255)                                                              cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                         >  1:501001/1‑140 (MQ=255)                                                              cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                         >  1:452104/1‑140 (MQ=255)                                                              cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                         >  1:302691/1‑140 (MQ=255)                                                              cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                         >  2:786681/1‑140 (MQ=255)                                                              cacCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCACCCTTCATGCGCAACGGAAGTAGCAACGTTgg                                                                         >  2:911496/1‑140 (MQ=255)                                                                  tctTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAAt                                                                     <  2:242012/140‑1 (MQ=255)                                                                      cgcgCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACg                                                                 >  2:408824/1‑140 (MQ=255)                                                                       gcgcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACgg                                                                <  1:786681/140‑1 (MQ=255)                                                                        cgcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGc                                                               <  1:93288/140‑1 (MQ=255)                                                                        cgcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGc                                                               <  2:302691/140‑1 (MQ=255)                                                                         gcCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGcc                                                              >  2:327946/1‑140 (MQ=255)                                                                           cGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAg                                                            >  1:692605/1‑140 (MQ=255)                                                                             ttCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgtt                                                          <  2:386990/140‑1 (MQ=255)                                                                                cAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgttgtt                                                       <  2:501001/140‑1 (MQ=255)                                                                                       tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAgg                                                >  1:683923/1‑140 (MQ=255)                                                                                       tGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAgg                                                <  2:791533/140‑1 (MQ=255)                                                                                                accagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCctgctg                                       <  2:693710/140‑1 (MQ=255)                                                                                                 ccagCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCtgctgc                                      <  2:692605/140‑1 (MQ=255)                                                                                                    gCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAg                                   >  1:170881/1‑140 (MQ=255)                                                                                                     cGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGt                                  >  2:343066/1‑140 (MQ=255)                                                                                                     cGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGt                                  >  2:85919/1‑140 (MQ=255)                                                                                                       aaCAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAgg                                                <  2:195733/124‑1 (MQ=255)                                                                                                       aaCAGCGTCCGTCAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAgg                                                >  1:195733/1‑124 (MQ=255)                                                                                                              tccgtcAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAAcgccgc                         >  2:231869/1‑140 (MQ=255)                                                                                                                   cAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAcgcg                    <  2:170881/140‑1 (MQ=255)                                                                                                                   cAGACATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCAcgcg                    <  1:790060/140‑1 (MQ=255)                                                                                                                      aCATCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTg                 >  1:382760/1‑140 (MQ=255)                                                                                                                         tCGGCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCt              <  2:382760/140‑1 (MQ=255)                                                                                                                            gCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCa                        >  1:917673/1‑127 (MQ=255)                                                                                                                            gCAACATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCa                        <  2:917673/127‑1 (MQ=255)                                                                                                                              aaCATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGc         <  1:343066/140‑1 (MQ=255)                                                                                                                              aaCATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCACCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGc         <  1:911496/140‑1 (MQ=255)                                                                                                                                cATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCa                        <  1:918301/123‑1 (MQ=255)                                                                                                                                cATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCa                        >  2:918301/1‑123 (MQ=255)                                                                                                                                cATCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTg       >  2:150335/1‑140 (MQ=255)                                                                                                                                  tCGCCAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGaa     <  1:150335/140‑1 (MQ=255)                                                                                                                                     ccAGCTGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATgg  <  2:610602/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTTGTTCGTAAGGCTCCTGCTGCCAGTCAACGCCGCACGCGGTGGCTAACGCTGAATGG  >  NC_000913/3052756‑3053027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑