Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,199,571 G→T A24D (GCC→GAC)  glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,199,5710GT93.3% 92.2 / 1.5 30A24D (GCC→GAC) glnEfused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (14/14);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

CGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGT  >  NC_000913/3199434‑3199704
                                                                                                                                         |                                                                                                                                     
cgTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCCGTTCCGTCCGCCACTCTTGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAATAAGTACTGACTTCGCCCGTGCGCTAAGTGATTCCTCGTCt                                                                                                                                     >  2:6996/1‑140 (MQ=255)
     tgctgcCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAgg                                                                                                                                <  2:556797/140‑1 (MQ=255)
               cgtcgGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGc                                                                                                                      <  2:451027/140‑1 (MQ=255)
                            cggTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACgg                                                                                                         <  1:640795/140‑1 (MQ=255)
                                    tttCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAg                                                                                                 <  1:762499/140‑1 (MQ=255)
                                              cGTCAGCCACTCTGGATGCGCAATCATGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTa                                                                                       <  1:194151/140‑1 (MQ=255)
                                                tCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTGAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAAc                                                                                     >  1:250029/1‑140 (MQ=255)
                                                tCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAAc                                                                                     >  2:440033/1‑140 (MQ=255)
                                                tCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTACCAGTAc                                                                                              <  1:333397/131‑1 (MQ=255)
                                                tCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGCAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTACCAGTAc                                                                                              >  2:333397/1‑131 (MQ=255)
                                                   gCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGt                                                                                  <  1:789356/140‑1 (MQ=255)
                                                              tGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCgg                                                                       <  2:626094/140‑1 (MQ=255)
                                                                      cACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATaa                                                               >  2:268794/1‑140 (MQ=255)
                                                                      cACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATaa                                                               >  2:630/1‑140 (MQ=255)
                                                                                     aaaaTCACTGAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTg                                                <  2:250029/140‑1 (MQ=255)
                                                                                       aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTa                                                      <  1:709106/132‑1 (MQ=255)
                                                                                       aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTa                                                      >  2:709106/1‑132 (MQ=255)
                                                                                       aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGcc                                              >  2:863148/1‑140 (MQ=255)
                                                                                       aaTCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGcc                                              >  2:199846/1‑140 (MQ=255)
                                                                                                     agtaCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTc                                >  1:708967/1‑140 (MQ=255)
                                                                                                     agtaCTGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTc                                <  1:440033/140‑1 (MQ=255)
                                                                                                          tGACTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCtg                           >  2:816042/1‑140 (MQ=255)
                                                                                                             cTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGc                        <  1:630/140‑1 (MQ=255)
                                                                                                             cTTCGCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGc                        <  1:268794/140‑1 (MQ=255)
                                                                                                                 gCCTGTGCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAAcc                    >  2:336006/1‑140 (MQ=255)
                                                                                                                      tgCGCTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAAc               >  1:885234/1‑140 (MQ=255)
                                                                                                                        cgcTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGc                        <  1:1054/129‑1 (MQ=255)
                                                                                                                        cgcTAAGTGATTCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGc                        >  2:1054/1‑129 (MQ=255)
                                                                                                                           tAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGaa                >  2:115913/1‑134 (MQ=255)
                                                                                                                           tAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGaa                <  1:115913/134‑1 (MQ=255)
                                                                                                                                   tCCTCGTCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGt  >  2:443108/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCCAGTTCCGTCAGCCACTCTGGATGCGCAATCACGCTGTCCTGCACAAAATCACTAAAAGTAAGTACTGACTTCGCCTGTGCGCTAAGTGATTCCTCGGCTAAAGGCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGT  >  NC_000913/3199434‑3199704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑