Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	125,737	G→T	V15F (GTT→TTT)	aceF →	pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	125,737	0	G	T	100.0%	204.3 / NA	58	V15F (GTT→TTT)	aceF	pyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/31);  total (27/31)

ATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATC  >  NC_000913/125603‑125865                                                                                                                                       |                                                                                                                                 aTCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGaa                                                                                                                             <  1:12549/140‑1 (MQ=255) aTCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGaa                                                                                                                             <  2:823171/140‑1 (MQ=255)     aTAAGAAAGTGGTTGTTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCa                                                                                                                         >  2:395461/1‑140 (MQ=255)      tAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCAc                                                                                                                        <  1:375120/140‑1 (MQ=255)          aaaGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCgag                                                                                                                    >  1:490014/1‑140 (MQ=255)            aGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGAt                                                                                                                  <  1:614110/140‑1 (MQ=255)            aGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGAt                                                                                                                  <  1:158933/140‑1 (MQ=255)                        cGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGt                                                                                                      >  1:547628/1‑140 (MQ=255)                                 cAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACaa                                                                                             >  2:268290/1‑140 (MQ=255)                                       cAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGa                                                                                       >  2:134342/1‑140 (MQ=255)                                            tCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATAAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCg                                                                                  <  2:89109/140‑1 (MQ=255)                                             cGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGa                                                                                 >  2:835757/1‑140 (MQ=255)                                               aTGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAc                                                                               >  2:396703/1‑140 (MQ=255)                                               aTGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAc                                                                               <  2:254686/140‑1 (MQ=255)                                                     aTAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGc                                                                         >  1:792136/1‑140 (MQ=255)                                                      tAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCt                                                                        <  1:268290/140‑1 (MQ=255)                                                         aGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGAt                                                                     <  1:395461/140‑1 (MQ=255)                                                         aGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGAt                                                                     <  2:194337/140‑1 (MQ=255)                                                          gTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGAt                                                                     >  1:287800/1‑139 (MQ=255)                                                          gTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGAt                                                                     <  2:287800/139‑1 (MQ=255)                                                              aCCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGaa                                                                                <  2:543020/124‑1 (MQ=255)                                                              aCCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGaa                                                                                >  1:543020/1‑124 (MQ=255)                                                              aCCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCg                                                                >  2:297279/1‑140 (MQ=255)                                                                    gTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAg                                                          >  1:57565/1‑140 (MQ=255)                                                                    gTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAg                                                          >  2:850495/1‑140 (MQ=255)                                                                    gTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAg                                                          >  1:143102/1‑140 (MQ=255)                                                                     tCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGa                                                                      <  2:139435/127‑1 (MQ=255)                                                                     tCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGa                                                                      >  1:139435/1‑127 (MQ=255)                                                                     tCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAgg                                                         >  1:143548/1‑140 (MQ=255)                                                                             aaGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAg                                                             <  2:951134/128‑1 (MQ=255)                                                                             aaGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAg                                                             >  1:951134/1‑128 (MQ=255)                                                                              agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                <  1:297279/140‑1 (MQ=255)                                                                              agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                <  2:378140/140‑1 (MQ=255)                                                                              agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                <  1:835757/140‑1 (MQ=255)                                                                              agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                <  2:73109/140‑1 (MQ=255)                                                                              agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGATGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                                >  1:498811/1‑140 (MQ=255)                                                                               gagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGcc                                               <  2:792136/140‑1 (MQ=255)                                                                                       aGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGa                                       >  2:290724/1‑140 (MQ=255)                                                                                        gAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTTGGCGACAGAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGaa                                      <  2:17031/140‑1 (MQ=255)                                                                                          ataatGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGt                                    <  2:29252/140‑1 (MQ=255)                                                                                           taatGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGtt                                   >  1:481781/1‑140 (MQ=255)                                                                                            aatGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTc                                  >  1:289684/1‑140 (MQ=255)                                                                                                   aTCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTcc                           >  1:836344/1‑140 (MQ=255)                                                                                                    tCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCg                          <  2:489368/140‑1 (MQ=255)                                                                                                    tCGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCg                          <  2:836344/140‑1 (MQ=255)                                                                                                     cGAAATCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGc                         >  1:95406/1‑140 (MQ=255)                                                                                                        aaTCAAAGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAgg                      <  2:289684/140‑1 (MQ=255)                                                                                                            aaaGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg                  <  2:481781/140‑1 (MQ=255)                                                                                                            aaaGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg                  <  2:547628/140‑1 (MQ=255)                                                                                                            aaaGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg                  <  2:143102/140‑1 (MQ=255)                                                                                                              aGTACCGGACATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTa                >  2:285428/1‑140 (MQ=255)                                                                                                                       cATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAag       <  1:396703/140‑1 (MQ=255)                                                                                                                       cATCGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGATGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAag       <  2:498811/140‑1 (MQ=255)                                                                                                                          cGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAag       <  1:356922/137‑1 (MQ=255)                                                                                                                          cGGGGCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTAACGTTAAag       >  2:356922/1‑137 (MQ=255)                                                                                                                            gggCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATc  >  1:755329/1‑140 (MQ=255)                                                                                                                            gggCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATc  <  2:755329/140‑1 (MQ=255)                                                                                                                            gggCTGATGAATTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGATAAAAAGGTc  >  2:659641/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 ATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATC  >  NC_000913/125603‑125865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑