Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,923	A→G	intergenic (‑104/‑166)	ybjL ← / → ybjM	putative transport protein YbjL/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,923	0	A	G	100.0%	189.3 / NA	52	intergenic (‑104/‑166)	ybjL/ybjM	putative transport protein YbjL/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

CCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAA  >  NC_000913/889787‑890055                                                                                                                                            |                                                                                                                                     cccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGa                                                                                                                                   <  2:324629/140‑1 (MQ=255)  ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                  <  2:407366/140‑1 (MQ=255)      ttAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATagta                                                                                                                              <  2:495257/140‑1 (MQ=255)      ttAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATagta                                                                                                                              >  1:348654/1‑140 (MQ=255)                  cGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatat                                                                                                                  >  1:745397/1‑140 (MQ=255)                  cGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatat                                                                                                                  >  1:7632/1‑140 (MQ=255)                  cGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTCGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatat                                                                                                                  >  2:549422/1‑140 (MQ=255)                     cGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                     >  2:260234/1‑134 (MQ=255)                     cGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                     <  1:260234/134‑1 (MQ=255)                        ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                            <  2:745397/140‑1 (MQ=255)                        ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                            <  2:348654/140‑1 (MQ=255)                        ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                            <  2:268434/140‑1 (MQ=255)                          atatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACt                                                                                                          <  2:355741/140‑1 (MQ=255)                                aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                           >  1:589032/1‑133 (MQ=255)                                aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                           <  2:589032/133‑1 (MQ=255)                                aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                    <  2:7632/140‑1 (MQ=255)                                 cGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                   <  1:484060/140‑1 (MQ=255)                                 cGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                   >  1:796109/1‑140 (MQ=255)                                  ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                  <  1:851355/140‑1 (MQ=255)                                  ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                  >  1:735419/1‑140 (MQ=255)                                  ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGT‑AAGTAACAATATATTTTACTAGTGTAATc                                                                                                 >  1:207416/1‑140 (MQ=255)                                      gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                  >  2:538105/1‑136 (MQ=255)                                      gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                  <  1:538105/136‑1 (MQ=255)                                      gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGT‑AAGTAACAATATATTTTACTAGTGTAATCACAt                                                                                             <  2:207416/140‑1 (MQ=255)                                       cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACAt                                                                                             >  2:273922/1‑140 (MQ=255)                                                tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                    >  1:637321/1‑140 (MQ=255)                                                tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                    >  2:743559/1‑140 (MQ=255)                                                tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                    <  2:637321/140‑1 (MQ=255)                                                    tACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACgg                                                                                >  2:64157/1‑140 (MQ=255)                                                              cTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAAt                                                                      >  2:651591/1‑140 (MQ=255)                                                              cTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAAt                                                                      >  2:706934/1‑140 (MQ=255)                                                                  tGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                  <  1:64157/140‑1 (MQ=255)                                                                   gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                 <  2:633435/140‑1 (MQ=255)                                                                      agaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTgg                                                              <  1:743559/140‑1 (MQ=255)                                                                       gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                             <  1:180914/140‑1 (MQ=255)                                                                          aTGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCta                                                          <  1:543583/140‑1 (MQ=255)                                                                           tGGTAATTTACGATAATGTTTTTTACCAGAATTCAGCTGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                            <  2:358921/140‑1 (MQ=255)                                                                           tGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                            >  1:704894/1‑137 (MQ=255)                                                                           tGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                            <  2:704894/137‑1 (MQ=255)                                                                            ggTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtata                                                        <  1:549422/140‑1 (MQ=255)                                                                            ggTAATTTACGATAATGTTTTTTACCAGAATACA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtata                                                        <  2:735419/140‑1 (MQ=255)                                                                                        tAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAAt                                            >  2:145653/1‑140 (MQ=255)                                                                                           tGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCg                                         >  2:907173/1‑140 (MQ=255)                                                                                             ttttttACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGaa                                       <  1:357496/140‑1 (MQ=255)                                                                                                        aaTTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATc                            >  2:215471/1‑140 (MQ=255)                                                                                                           tCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCTCATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTa                         <  1:189686/140‑1 (MQ=255)                                                                                                             a‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACt                       <  2:114077/140‑1 (MQ=255)                                                                                                             a‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACt                       <  2:796109/140‑1 (MQ=255)                                                                                                                      cAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGc                 >  2:430676/1‑140 (MQ=255)                                                                                                                                 aGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGa      <  1:215471/140‑1 (MQ=255)                                                                                                                                   cgcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaa    >  2:945800/1‑140 (MQ=255)                                                                                                                                     cACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaaaa  >  2:634659/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                     CCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCA‑‑‑GGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAA  >  NC_000913/889787‑890055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑