Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,215	0	T	G	25.0%	144.7 / 19.0	64	G244G (GGT→GGG)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (8/40);  new base G (16/0);  total (24/40)
Fisher's exact test for biased strand distribution p-value = 1.51e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCT  >  NC_000913/3404083‑3404354                                                                                                                                     |                                                                                                                                             gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                                                                                                       <  1:985071/140‑1 (MQ=255) gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                                                                                                       <  1:34725/140‑1 (MQ=255) gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGgtttt                                                                                                                                       >  2:1089860/1‑139 (MQ=255)  tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCggggggggtttt                                                                                                                                      >  2:1147686/1‑134 (MQ=255)  tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGgttttc                                                                                                                                      >  2:34725/1‑138 (MQ=255)      ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                                  <  1:38398/140‑1 (MQ=255)        gAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGcc                                                                                                                                <  1:1131960/140‑1 (MQ=255)        gAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGcc                                                                                                                                <  2:766543/140‑1 (MQ=255)          aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCaa                                                                                                                              >  2:354327/1‑140 (MQ=255)           tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTTCCAAc                                                                                                                             >  1:977491/1‑140 (MQ=255)             ccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCa                                                                                                                           <  2:630071/140‑1 (MQ=255)                tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTTCTCTGCCCACCACgg                                                                                                                        >  2:514355/1‑140 (MQ=255)                tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGGGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACgg                                                                                                                        >  1:336623/1‑140 (MQ=255)                    ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:906122/140‑1 (MQ=255)                    ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:1089860/140‑1 (MQ=255)                    agggAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                    <  1:405415/138‑1 (MQ=255)                     tGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGGGGGCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCAT‑g                                                                                                                  >  2:906122/1‑140 (MQ=255)                       gAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGGCAT‑Gcc                                                                                                                >  2:1025731/1‑140 (MQ=255)                        aaaaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCa                                                                                                               <  1:1288030/140‑1 (MQ=255)                        aaaaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCa                                                                                                               <  1:364502/140‑1 (MQ=255)                            aCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCa                                                                                                                           >  1:35379/1‑125 (MQ=255)                            aCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCa                                                                                                                           <  2:35379/125‑1 (MQ=255)                            aCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTa                                                                                                           >  1:296467/1‑140 (MQ=255)                            aCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTTCAACCACGGGCCT‑GCCAtttt                                                                                                           >  2:865535/1‑139 (MQ=255)                                 tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCAt                                                                                                              <  2:480835/132‑1 (MQ=255)                                 tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCAT‑GCCAt                                                                                                              >  1:480835/1‑132 (MQ=255)                                 tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCAT‑GCCCtttttttct                                                                                                      >  2:405415/1‑137 (MQ=255)                                   tgggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCg                                                                                                    <  1:1236980/140‑1 (MQ=255)                                   tgggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCg                                                                                                    <  1:514355/140‑1 (MQ=255)                                      gctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTaa                                                                                                 <  1:1086894/140‑1 (MQ=255)                                          gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGGCAT‑GCCCTTTTTTTTGCGGAATGTc                                                                                             >  2:1086894/1‑140 (MQ=255)                                           ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGTCAT‑GGCCTTTTTTTTGCGGAATGttc                                                                                            >  2:966121/1‑138 (MQ=255)                                           ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCCCGGGCAT‑GCCCTTTTTTTTTCGGAATGTcc                                                                                            >  2:1173542/1‑140 (MQ=255)                                                ttGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTg                                                                                       >  1:873419/1‑140 (MQ=255)                                                ttGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCCCCGTCAT‑GCCCTTTTTTTTGCGTAATGTCCGtttt                                                                                       >  2:1258874/1‑139 (MQ=255)                                                ttGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCAT‑GCCATTTTTTCTGCGTAATGTCCGtttt                                                                                       >  2:512820/1‑139 (MQ=255)                                                  gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCa                                                                                     <  1:865535/140‑1 (MQ=255)                                                      ccGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTAATCTGCCAACCACGGGCAT‑GCCATTTATTCTGCGTAATGTCCGCTTGCAAagg                                                                                 >  2:499367/1‑140 (MQ=255)                                                        gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                               <  1:1027981/140‑1 (MQ=255)                                                        gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                               <  2:1046745/140‑1 (MQ=255)                                                        gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                               <  1:371595/140‑1 (MQ=255)                                                         ttGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTg                                                                              <  1:1096082/140‑1 (MQ=255)                                                          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGGCAT‑GCCATTTATTTTGCGTAATGTCCGTTTGCAAGgggggg                                                                             >  1:675868/1‑140 (MQ=255)                                                          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCAT‑GCCATTTATTTTGCGTAATGTCCGTTTGCAAGgggggg                                                                             >  2:864491/1‑140 (MQ=255)                                                            gCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGAt                                                                           <  1:864491/140‑1 (MQ=255)                                                                   aGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTaa                                                                    >  1:1150139/1‑140 (MQ=255)                                                                         ggCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCATCCGCGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGc                                                              <  2:70578/140‑1 (MQ=255)                                                                         ggCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGc                                                              <  1:1193768/140‑1 (MQ=255)                                                                         ggCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGc                                                              <  2:1150139/140‑1 (MQ=255)                                                                               aGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAg                                                        >  1:830781/1‑140 (MQ=255)                                                                                 tGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGaa                                                      <  2:830781/140‑1 (MQ=255)                                                                                    tGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCCACCACCGGCAT‑GCCCTTTTTTTTGCGTAATGTCCGTTTTCAAAGGGGGGGTTCAGTAATGAAGCCCCCCGAAcgc                                                   >  2:325467/1‑140 (MQ=255)                                                                                    tGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCCGTGGGCTGGCGGGGGGGTTTTCTCTGCCCACCACGGTCATGGCCATTT‑TTCTGCCTAATTTCCGGTTTCAAAGGGGGGATTCAATAATTACGCCACCAGAAagc                                                   >  2:285601/1‑140 (MQ=255)                                                                                     ggCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgcc                                                  <  2:1002721/140‑1 (MQ=255)                                                                                      gcgcAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTCCTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgccg                                                 <  2:151164/140‑1 (MQ=255)                                                                                      gcgcAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgccg                                                 <  1:853348/140‑1 (MQ=255)                                                                                           aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                            <  1:285601/140‑1 (MQ=255)                                                                                                 ttACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCt                                      >  1:151164/1‑140 (MQ=255)                                                                                                    cggcggCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCAT‑GCCCTTTATTCTGCGGGATGTCCGTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGc                                   >  1:1195440/1‑140 (MQ=255)                                                                                                      gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACCGGCAT‑GCCATTTATTTTGCGTAATGTCCGTTTGCAAGGGGGGGGTTTAGTAATGACGCCCCCCGAACGCCGCGCACAAACCCGGCAg                                 >  2:288188/1‑140 (MQ=255)                                                                                                         gcTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACACCGCGCACAAGCCTGGCAGCGa                              <  2:296467/140‑1 (MQ=255)                                                                                                          cTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGAc                             <  1:1025731/140‑1 (MQ=255)                                                                                                                       tgtgGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGAttt                >  2:693219/1‑140 (MQ=255)                                                                                                                            tCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCgg           >  1:845746/1‑140 (MQ=255)                                                                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:325467/140‑1 (MQ=255)                                                                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  2:1195440/140‑1 (MQ=255)                                                                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:354327/140‑1 (MQ=255)                                                                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         <  1:512820/140‑1 (MQ=255)                                                                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa         >  1:891247/1‑140 (MQ=255)                                                                                                                               ggCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  1:288188/140‑1 (MQ=255)                                                                                                                               ggCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  2:845746/140‑1 (MQ=255)                                                                                                                               ggCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt        <  1:77780/140‑1 (MQ=255)                                                                                                                                gCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       <  1:456741/140‑1 (MQ=255)                                                                                                                                 cGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       >  2:378955/1‑139 (MQ=255)                                                                                                                                 cGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATc       <  1:378955/139‑1 (MQ=255)                                                                                                                                 cGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCa      >  1:199823/1‑140 (MQ=255)                                                                                                                                     tggtTTTACTCTGCCCACCACGGGCAT‑GCCATTTATTCTGCGTAATGGCCGTTTTGAAAGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCCGGCAGCGACTGGTCGCCCATTTAACGGAATCCTTCt  >  2:51013/1‑140 (MQ=255)                                                                                                                                     tggtTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt  >  2:684851/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                             GTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAT‑GCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCT  >  NC_000913/3404083‑3404354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑