Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,081,905	G→T	Q215K (CAA→AAA)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,081,905	0	G	T	100.0%	207.8 / NA	59	Q215K (CAA→AAA)	dacD	D‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/31);  total (28/31)

CATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAGGTGCATCCAGA  >  NC_000913/2081771‑2082035                                                                                                                                       |                                                                                                                                   cATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTggg                                                                                                                               <  2:405528/140‑1 (MQ=255)   ttACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTg                                                                                                                             >  2:765537/1‑140 (MQ=255)   ttACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTg                                                                                                                             >  1:819377/1‑140 (MQ=255)          tGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGt                                                                                                                      >  2:114342/1‑140 (MQ=255)          tGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGACACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGt                                                                                                                      >  2:992507/1‑140 (MQ=255)            cAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTc                                                                                                                    >  2:1031539/1‑140 (MQ=255)               tGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAg                                                                                                                 >  2:87674/1‑140 (MQ=255)                gagaCGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAgg                                                                                                                <  1:1140753/140‑1 (MQ=255)                   acgacgCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTga                                                                                                             >  2:889219/1‑140 (MQ=255)                        gCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACtt                                                                                                        <  2:69251/140‑1 (MQ=255)                            cccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  2:819377/140‑1 (MQ=255)                            cccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  2:211797/140‑1 (MQ=255)                            cccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  1:768495/140‑1 (MQ=255)                            cccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  1:765537/140‑1 (MQ=255)                             ccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtctc                                                                                                   <  1:636019/140‑1 (MQ=255)                              cATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCa                                                                                                  <  1:889219/140‑1 (MQ=255)                                      gCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    >  1:163631/1‑130 (MQ=255)                                      gCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  2:163631/130‑1 (MQ=255)                                      gCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    >  2:1146430/1‑130 (MQ=255)                                      gCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  1:1146430/130‑1 (MQ=255)                                      gCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACat                                                                                          <  1:597567/140‑1 (MQ=255)                                        cGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACatat                                                                                        <  1:1031539/140‑1 (MQ=255)                                          aaGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  2:1026060/126‑1 (MQ=255)                                          aaGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    >  1:1026060/1‑126 (MQ=255)                                          aaGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGa                                                                                      >  2:739486/1‑140 (MQ=255)                                           aGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    <  2:245675/125‑1 (MQ=255)                                           aGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTtct                                                                                                    >  1:245675/1‑125 (MQ=255)                                           aGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACat                                                                                          <  1:697775/135‑1 (MQ=255)                                           aGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACat                                                                                          >  2:697775/1‑135 (MQ=255)                                                       aaaCCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCt                                                                         <  1:1173683/140‑1 (MQ=255)                                                        aaCCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTc                                                                        >  1:46808/1‑140 (MQ=255)                                                             ggCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCggg                                                                           <  1:82003/132‑1 (MQ=255)                                                             ggCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCggg                                                                           >  2:82003/1‑132 (MQ=255)                                                              gCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTg                                                                  >  1:176107/1‑140 (MQ=255)                                                                        tATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCa                                                        <  1:254404/140‑1 (MQ=255)                                                                            aCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACgag                                                    >  2:354093/1‑140 (MQ=255)                                                                                 ttttCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGc                                               <  1:992507/140‑1 (MQ=255)                                                                                       ggCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCt                                         <  1:1075290/140‑1 (MQ=255)                                                                                       ggCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCt                                         <  1:739486/140‑1 (MQ=255)                                                                                            tCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATc                                    >  1:119008/1‑140 (MQ=255)                                                                                                      tGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAg                          >  1:464306/1‑140 (MQ=255)                                                                                                      tGGTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAg                          >  2:455104/1‑140 (MQ=255)                                                                                                       ggTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAg                          >  2:1268994/1‑139 (MQ=255)                                                                                                       ggTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAg                          <  1:1268994/139‑1 (MQ=255)                                                                                                       ggTTTTATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTAGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGc                         >  2:259372/1‑140 (MQ=255)                                                                                                         ttttATCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTa                       <  2:176107/140‑1 (MQ=255)                                                                                                              tCCCACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTg                  <  2:119008/140‑1 (MQ=255)                                                                                                                ccACAACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGc                <  2:46808/140‑1 (MQ=255)                                                                                                                 cacaACAACCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGcc               >  2:540341/1‑140 (MQ=255)                                                                                                                    aacaacCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAgg            <  1:354093/140‑1 (MQ=255)                                                                                                                    aacaacCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAgg            <  1:24837/140‑1 (MQ=255)                                                                                                                       aacCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAgg            >  2:1238804/1‑137 (MQ=255)                                                                                                                       aacCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAgg            <  1:1238804/137‑1 (MQ=255)                                                                                                                        acCCGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAGGTGCa        >  1:373803/1‑140 (MQ=255)                                                                                                                         cccGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATg                     >  2:249692/1‑126 (MQ=255)                                                                                                                         cccGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATg                     <  1:249692/126‑1 (MQ=255)                                                                                                                           cGTTACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAGGTGCATcc     <  1:455104/140‑1 (MQ=255)                                                                                                                             ttACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAGGTGCATCCAg   >  1:669927/1‑140 (MQ=255)                                                                                                                              tACGGTTTTTCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAGGTGCATCCAGa  >  2:1158734/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   CATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTGGATGATAGCACGAGAAAGCACAGCTAAATCATAAGCCGAGCTATGCTGGCCAGGTGCATCCAGA  >  NC_000913/2081771‑2082035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑