Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,699	C→A	intergenic (‑156/‑102)	purL ← / → mltF	phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,699	0	C	A	100.0%	264.7 / NA	75	intergenic (‑156/‑102)	purL/mltF	phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/40);  total (35/40)

TTCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGG  >  NC_000913/2695565‑2695835                                                                                                                                       |                                                                                                                                          ttCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGgcg                                                                                                                                      >  1:150298/1‑140 (MQ=255) ttCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGgcg                                                                                                                                      >  1:893974/1‑140 (MQ=255)    ggTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAg                                                                                                                                   >  2:1156494/1‑140 (MQ=255)      tgtACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGt                                                                                                                                 <  2:539875/140‑1 (MQ=255)        tACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCGCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGttt                                                                                                                               <  2:1210108/140‑1 (MQ=255)        tACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGttt                                                                                                                               <  1:1156494/140‑1 (MQ=255)             aaGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCa                                                                                                                          <  1:585011/140‑1 (MQ=255)                ggggggAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCaaaa                                                                                                                       >  1:424222/1‑140 (MQ=255)                ggggggAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCaaaa                                                                                                                       >  2:104187/1‑140 (MQ=255)                ggggggAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCATGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCaaaa                                                                                                                       >  1:778673/1‑140 (MQ=255)                    ggAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGcc                                                                                                                   >  2:728602/1‑140 (MQ=255)                    ggAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGcc                                                                                                                   >  1:882954/1‑140 (MQ=255)                            gCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCAttt                                                                                                           <  2:60129/140‑1 (MQ=255)                            gCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCAttt                                                                                                           >  2:1207824/1‑140 (MQ=255)                                   tataAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTa                                                                                                    >  2:1105154/1‑140 (MQ=255)                                   tataAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTa                                                                                                    <  2:800188/140‑1 (MQ=255)                                          aaTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑cc                                                                                               <  2:1054056/137‑1 (MQ=255)                                          aaTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑cc                                                                                               >  1:1054056/1‑137 (MQ=255)                                          aaTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCttt                                                                                            <  2:424222/140‑1 (MQ=255)                                          aaTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCttt                                                                                            <  2:150298/140‑1 (MQ=255)                                          aaTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCttt                                                                                            >  1:1125359/1‑140 (MQ=255)                                          aaTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCttt                                                                                            <  2:893974/140‑1 (MQ=255)                                           aTCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTc                                                                                           >  2:987194/1‑140 (MQ=255)                                            tCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCttt                                                                                            <  2:847887/138‑1 (MQ=255)                                            tCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCttt                                                                                            >  1:847887/1‑138 (MQ=255)                                            tCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCa                                                                                          >  2:142742/1‑140 (MQ=255)                                            tCTGATGCGCTGACGAAACCGTTTGCGAGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGTCGCCAAAGGCTGTAATAAGTTGCCATATGGCGCAGGGTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCa                                                                                          >  2:33479/1‑140 (MQ=255)                                             cTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCAc                                                                                         <  1:524451/140‑1 (MQ=255)                                             cTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCAc                                                                                         <  1:1207824/140‑1 (MQ=255)                                               gATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACtt                                                                                       >  1:305402/1‑139 (MQ=255)                                               gATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACta                                                                                       >  2:212692/1‑140 (MQ=255)                                                            aaCCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGttttt                                                                          <  2:1117683/140‑1 (MQ=255)                                                              ccGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTc                                                                        <  1:1180532/140‑1 (MQ=255)                                                                    gCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTg                                                                  >  2:190320/1‑140 (MQ=255)                                                                      gTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTggg                                                                <  2:882954/140‑1 (MQ=255)                                                                      gTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTggg                                                                <  1:142742/140‑1 (MQ=255)                                                                      gTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTggg                                                                <  1:472619/140‑1 (MQ=255)                                                                          aaaTAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGAcgc                                                            <  1:728602/140‑1 (MQ=255)                                                                           aaTAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGAcgcg                                                           >  2:1271886/1‑140 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCa                                                         >  2:409316/1‑134 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCa                                                         <  1:409316/134‑1 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCCTTTCACTATTATTACGTTTTTTCAAGCCGGGACGCGCa                                                         <  1:522325/134‑1 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAACCCTTTCACTATTATTACGTTTTTTCAAGCCGGGACGCGCa                                                         >  2:522325/1‑134 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTggg                                                                >  1:812719/1‑126 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTggg                                                                <  2:812719/126‑1 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGAcaca                                                  <  1:987194/140‑1 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGAcaca                                                  >  2:357911/1‑140 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGAcaca                                                  <  1:1105154/140‑1 (MQ=255)                                                                                    aCCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGAcaca                                                  <  1:104187/140‑1 (MQ=255)                                                                                         cGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGacac                                                   <  2:644686/134‑1 (MQ=255)                                                                                         cGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGacac                                                   >  1:644686/1‑134 (MQ=255)                                                                                          gTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACgcgc                                                          <  1:1057809/126‑1 (MQ=255)                                                                                          gTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACgcgc                                                          >  2:1057809/1‑126 (MQ=255)                                                                                                   cAACGCGTGCCGCCAATGGCTGTGATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTaa                                         <  2:206170/134‑1 (MQ=255)                                                                                                   cAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTaa                                         >  1:206170/1‑134 (MQ=255)                                                                                                      cgcgTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGaa                                >  2:481160/1‑140 (MQ=255)                                                                                                      cgcgTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGaa                                >  2:750283/1‑140 (MQ=255)                                                                                                       gcgTGCCGCCAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGaaa                               >  2:1130413/1‑140 (MQ=255)                                                                                                             cgccAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTa                         <  1:357911/140‑1 (MQ=255)                                                                                                             cgccAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTa                         <  1:212692/140‑1 (MQ=255)                                                                                                               ccAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTaaa                       <  2:778673/140‑1 (MQ=255)                                                                                                               ccAATGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTaaa                       <  1:33479/140‑1 (MQ=255)                                                                                                                  aTGGCTGTAATAAGTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGat                    <  1:481160/140‑1 (MQ=255)                                                                                                                               gTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTa                         >  2:253245/1‑122 (MQ=255)                                                                                                                               gTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTa                         <  1:253245/122‑1 (MQ=255)                                                                                                                               gTTGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTc       >  1:504539/1‑140 (MQ=255)                                                                                                                                 tGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGat                    >  2:208430/1‑125 (MQ=255)                                                                                                                                 tGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGat                    <  1:208430/125‑1 (MQ=255)                                                                                                                                 tGCCATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCAt     >  1:1133655/1‑140 (MQ=255)                                                                                                                                   ccATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTAt             <  1:874242/130‑1 (MQ=255)                                                                                                                                   ccATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTAt             >  2:874242/1‑130 (MQ=255)                                                                                                                                    cATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCgg  <  2:504539/140‑1 (MQ=255)                                                                                                                                    cATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCgg  <  2:1133655/140‑1 (MQ=255)                                                                                                                                    cATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCgg  <  1:1211339/140‑1 (MQ=255)                                                                                                                                    cATATGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCgg  <  1:190320/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                          TTCGGTGTACCCAAGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATGCCGCTCATTTATGAGTAAA‑CCTTTCACTATTATTACGTTTTTTCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGG  >  NC_000913/2695565‑2695835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑