Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,367,598	C→A	E244* (GAG→TAG)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,367,598	0	C	A	100.0%	209.0 / NA	59	E244* (GAG→TAG)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/28);  total (31/28)

CCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGT  >  NC_000913/4367466‑4367733                                                                                                                                     |                                                                                                                                        ccAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGc                                                                                                                                  <  2:994085/140‑1 (MQ=255) ccAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGc                                                                                                                                  <  2:783766/140‑1 (MQ=255) ccAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGc                                                                                                                                  <  2:1161475/140‑1 (MQ=255) ccAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGc                                                                                                                                  <  1:657868/140‑1 (MQ=255)   aGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCgt                                                                                                                                >  2:1153043/1‑140 (MQ=255)   aGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCgt                                                                                                                                >  1:637596/1‑140 (MQ=255)    ggCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGc                                                                                                                                  >  2:890538/1‑137 (MQ=255)    ggCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGc                                                                                                                                  <  1:890538/137‑1 (MQ=255)      cGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGtt                                                                                                                             <  1:396791/140‑1 (MQ=255)           cAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGAc                                                                                                                        <  1:983000/140‑1 (MQ=255)             gcgcgcCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCa                                                                                                                      <  1:721979/140‑1 (MQ=255)               gcgcCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGt                                                                                                                    >  1:983355/1‑140 (MQ=255)               gcgcCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGt                                                                                                                    <  2:498917/140‑1 (MQ=255)                       aaCCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTg                                                                                                            <  2:351055/140‑1 (MQ=255)                            taacataaGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGCACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGtt                                                                                                       <  2:699670/140‑1 (MQ=255)                             aacataaGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTTACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTg                                                                                                      >  1:674621/1‑140 (MQ=255)                                 taaGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCAcc                                                                                                  >  2:608366/1‑140 (MQ=255)                                 taaGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCAcc                                                                                                  >  2:340054/1‑140 (MQ=255)                                    gcgcCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCTGACCAGTACCGATTGCTGTTTCACCAAg                                                                                               >  2:1092639/1‑140 (MQ=255)                                    gcgcCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAg                                                                                               >  2:709703/1‑140 (MQ=255)                                      gccgcAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGt                                                                                             <  2:1191016/140‑1 (MQ=255)                                         gcAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTaa                                                                                          >  1:570433/1‑140 (MQ=255)                                         gcAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTaa                                                                                          <  2:983355/140‑1 (MQ=255)                                          cAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAAc                                                                                         >  1:1252678/1‑140 (MQ=255)                                              cAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTcc                                                                                     <  2:1252678/140‑1 (MQ=255)                                                 agGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCcagc                                                                                  <  1:608366/140‑1 (MQ=255)                                                   gTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCcagcag                                                                                <  1:437613/140‑1 (MQ=255)                                                      gCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGTCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCcagcagcag                                                                             >  1:1191597/1‑140 (MQ=255)                                                      gCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCcagcagcag                                                                             >  2:146770/1‑140 (MQ=255)                                                       cTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGt                                                                            >  1:679614/1‑140 (MQ=255)                                                       cTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGt                                                                            >  2:131248/1‑140 (MQ=255)                                                        ttCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGtt                                                                           <  2:679614/140‑1 (MQ=255)                                                              cAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCgg                                                                     <  1:1153043/140‑1 (MQ=255)                                                                      cAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTgg                                                             <  1:146770/140‑1 (MQ=255)                                                                               cGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGTTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTc                                                    <  2:137741/140‑1 (MQ=255)                                                                                   tGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGCACTATTTCGGCGTGTTCAGACCAGTACCGATTCCTGTTGCACCAAGGTTAACTTCCAGCAGCCGTTCAGCGGTACGTTGGATGTTTTTCActt                                                >  2:779602/1‑140 (MQ=255)                                                                                       aaGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCActtcttc                                            >  1:420870/1‑140 (MQ=255)                                                                                       aaGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCActtcttc                                            >  1:184851/1‑140 (MQ=255)                                                                                       aaGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCActtcttc                                            >  1:8924/1‑140 (MQ=255)                                                                                            aGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTca                                       >  1:1046009/1‑140 (MQ=255)                                                                                              tAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTcagc                                     <  2:8924/140‑1 (MQ=255)                                                                                              tAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACATTGGATGTTTTTCACTTCTTCTTTcagc                                     >  1:411682/1‑140 (MQ=255)                                                                                                aCTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTcagcag                                   >  1:683234/1‑140 (MQ=255)                                                                                                  ttCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGa                                 <  2:544382/140‑1 (MQ=255)                                                                                                    cAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCttt                                         >  2:70543/1‑130 (MQ=255)                                                                                                    cAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCttt                                         <  1:70543/130‑1 (MQ=255)                                                                                                       ccAGTTTTTTCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTg                            >  1:227338/1‑140 (MQ=255)                                                                                                           ttttttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAg                        <  2:184851/140‑1 (MQ=255)                                                                                                           ttttttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAg                        <  2:1191597/140‑1 (MQ=255)                                                                                                           ttttttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAg                        >  1:260193/1‑140 (MQ=255)                                                                                                            tttttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAgc                       >  1:422349/1‑140 (MQ=255)                                                                                                            tttttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAgc                       >  1:527467/1‑140 (MQ=255)                                                                                                             ttttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAgcg                      <  2:637596/140‑1 (MQ=255)                                                                                                               ttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGcgcg                    <  1:450016/140‑1 (MQ=255)                                                                                                               ttCACTGCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGcgcg                    <  2:420870/140‑1 (MQ=255)                                                                                                                     gCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTc              >  2:81731/1‑140 (MQ=255)                                                                                                                     gCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTc              >  1:757136/1‑140 (MQ=255)                                                                                                                     gCCAGCGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTc              >  1:1125708/1‑140 (MQ=255)                                                                                                                          cGGAGAGTACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGAc         >  1:975817/1‑140 (MQ=255)                                                                                                                                 tACTATTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGt  <  2:975817/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCAGCCGGTACGCATGGGAAGCCAGTAACTTCAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGCGGAATTCCTGACCGAGGGT  >  NC_000913/4367466‑4367733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑