Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,054	C→A	T618T (ACC→ACA)	yagF →	CP4‑6 prophage; D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,054	0	C	A	100.0%	241.8 / NA	70	T618T (ACC→ACA)	yagF	CP4‑6 prophage; D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/41);  total (29/41)

CGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATA  >  NC_000913/284917‑285183                                                                                                                                          |                                                                                                                                  cGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACg                                                                                                                                 >  1:811188/1‑140 (MQ=255)   tCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGc                                                                                                                               >  2:1048496/1‑140 (MQ=255)   tCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGc                                                                                                                               >  1:659365/1‑140 (MQ=255)   tCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGc                                                                                                                               >  1:178095/1‑140 (MQ=255)   tCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGc                                                                                                                               >  1:459282/1‑140 (MQ=255)   tCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGc                                                                                                                               >  1:224392/1‑140 (MQ=255)    cTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCt                                                                                                                              <  1:968525/140‑1 (MQ=255)          ttAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGggc                                                                                                                        <  1:154057/140‑1 (MQ=255)            aaCTGGCAGCGTGAACTTCATCGGCACAGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCGGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGggcgg                                                                                                                      <  1:142577/140‑1 (MQ=255)             aCTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCATGACTTTTTGCCGGACGACACACGGCTGTGggcggc                                                                                                                     <  1:1059260/140‑1 (MQ=255)                 gCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTg                                                                                                                 >  1:1277987/1‑140 (MQ=255)                    gCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAg                                                                                                              >  2:378957/1‑140 (MQ=255)                          aCTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTg                                                                                                        <  1:685744/140‑1 (MQ=255)                           cTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGa                                                                                                       >  1:298592/1‑140 (MQ=255)                               aTCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcgg                                                                                                   >  2:424823/1‑140 (MQ=255)                                tCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcggc                                                                                                  <  2:659365/140‑1 (MQ=255)                                tCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcggc                                                                                                  <  1:378957/140‑1 (MQ=255)                                   gCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCggcggc                                                                                               <  1:1048496/140‑1 (MQ=255)                                    cACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCa                                                                                              >  1:322818/1‑140 (MQ=255)                                      ccGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCAcc                                                                                            >  1:864546/1‑140 (MQ=255)                                      ccGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCAcc                                                                                            >  2:366184/1‑140 (MQ=255)                                      ccGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCAcc                                                                                            >  2:123366/1‑140 (MQ=255)                                         cgGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTgg                                                                                         >  1:848351/1‑140 (MQ=255)                                         cgGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTgg                                                                                         >  2:89206/1‑140 (MQ=255)                                           gACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGGCTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGaa                                                                                       <  2:339388/140‑1 (MQ=255)                                              aaCCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAgg                                                                                    <  1:960179/140‑1 (MQ=255)                                              aaCCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAgg                                                                                    <  2:811188/140‑1 (MQ=255)                                              aaCCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAgg                                                                                    <  1:1274715/140‑1 (MQ=255)                                              aaCCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAgg                                                                                    <  1:583746/140‑1 (MQ=255)                                              aaCCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAgg                                                                                    <  1:424823/140‑1 (MQ=255)                                                cccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  2:864546/140‑1 (MQ=255)                                                cccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  2:322818/140‑1 (MQ=255)                                                cccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  2:894468/140‑1 (MQ=255)                                                cccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  2:848351/140‑1 (MQ=255)                                                cccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  1:89206/140‑1 (MQ=255)                                                cccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTACAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  2:839924/140‑1 (MQ=255)                                                 ccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcggc                                                                                                  >  2:879424/1‑123 (MQ=255)                                                 ccGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcggc                                                                                                  <  1:879424/123‑1 (MQ=255)                                                  cGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGt                                                                                <  1:479284/140‑1 (MQ=255)                                                  cGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGt                                                                                <  1:1100015/140‑1 (MQ=255)                                                    cTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGaa                                                                                       >  1:214788/1‑131 (MQ=255)                                                    cTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGaa                                                                                       <  2:214788/131‑1 (MQ=255)                                                        cGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTAt                                                                          >  1:705134/1‑140 (MQ=255)                                                          ccGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGa                                                                        >  2:40272/1‑140 (MQ=255)                                                           cGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  >  1:366710/1‑129 (MQ=255)                                                           cGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCt                                                                                  <  2:366710/129‑1 (MQ=255)                                                                 agGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGAt                                                                 >  1:155678/1‑140 (MQ=255)                                                                    gcgcgcgcGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATaaa                                                              <  1:593542/140‑1 (MQ=255)                                                                    gcgcgcgcGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATaaa                                                              <  1:593942/140‑1 (MQ=255)                                                                        gcgcgAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTa                                                          >  1:214469/1‑140 (MQ=255)                                                                          gcgAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATc                                                        <  2:459282/140‑1 (MQ=255)                                                                           cgAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCg                                                       >  1:202145/1‑140 (MQ=255)                                                                             cggtGGGGCGGCGGCAGAGGCACCCCCACCTGCGCGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAg                                                     <  2:1080799/137‑1 (MQ=255)                                                                             aGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAg                                                     <  1:173447/140‑1 (MQ=255)                                                                             aGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAg                                                     <  2:705134/140‑1 (MQ=255)                                                                             aGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAg                                                     <  1:40272/140‑1 (MQ=255)                                                                                        cggcAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGc                                          >  2:140959/1‑140 (MQ=255)                                                                                        cggcAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGc                                          <  2:330482/140‑1 (MQ=255)                                                                                        cggcAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGc                                          >  1:992646/1‑140 (MQ=255)                                                                                                  aCCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcgg                                                                                                   <  1:593856/73‑1 (MQ=255)                                                                                                  aCCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAgcgg                                                                                                   >  2:593856/1‑73 (MQ=255)                                                                                                       gACCTGCACGCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCt                           <  2:992646/140‑1 (MQ=255)                                                                                                                gCCCACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTa                  <  2:214469/140‑1 (MQ=255)                                                                                                                 cccACGACTTTTTGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTaa                 <  2:1272759/140‑1 (MQ=255)                                                                                                                         tttttGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTa         <  2:298592/140‑1 (MQ=255)                                                                                                                         tttttGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTa         <  1:140959/140‑1 (MQ=255)                                                                                                                          ttttGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTaa        <  2:178095/140‑1 (MQ=255)                                                                                                                           tttGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAag       <  2:155678/140‑1 (MQ=255)                                                                                                                            ttGCCGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAaga      <  2:202145/140‑1 (MQ=255)                                                                                                                                cGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATa  >  1:569826/1‑140 (MQ=255)                                                                                                                                cGGACGACACACGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATa  >  1:676534/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  CGTCTGACGTTAACTGGCAGCGTGAACTTCATCGGCACCGCGGACAACCCGCTGACGCCGGAAGAGGGCGCGCGCGAGCTGGCGCGGCGGCAGACGCACCCGGACCTGCACGCCCACGACTTTTTGCCGGACGACACCCGGCTGTGGGCGGCACTGCAGTCGGTGAGCGGCGGCACCTGGAAAGGCTGTATTTATGACACCGATAAAATTATCGAGGTAATTAACGCCGGTAAAAAAGCGCTCGGAATTTAATTATTTTAAGAGATA  >  NC_000913/284917‑285183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑