Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	658,820	G→T	intergenic (+2/‑127)	ybeM → / → tatE	deamidated glutathione amidase, N‑terminal fragment/twin arginine protein translocation system ‑ TatE protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	658,820	0	G	T	100.0%	231.4 / NA	66	intergenic (+2/‑127)	ybeM/tatE	deamidated glutathione amidase, N‑terminal fragment/twin arginine protein translocation system ‑ TatE protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/36);  total (30/36)

CGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGA  >  NC_000913/658686‑658956                                                                                                                                       |                                                                                                                                         cGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATtttttt                                                                                                                                     <  2:389336/140‑1 (MQ=255)     aCCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTAc                                                                                                                                 >  2:1098257/1‑140 (MQ=255)     aCCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTAc                                                                                                                                 >  1:858780/1‑140 (MQ=255)      ccATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACt                                                                                                                                <  1:1265376/140‑1 (MQ=255)          tGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGgc                                                                                                                            >  1:720616/1‑140 (MQ=255)          tGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGgc                                                                                                                            >  1:1292435/1‑140 (MQ=255)                 gCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGAt                                                                                                                     >  2:725777/1‑140 (MQ=255)                 gCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGAt                                                                                                                     >  2:725325/1‑140 (MQ=255)                  cGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGAtt                                                                                                                    <  1:540183/140‑1 (MQ=255)                  cGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGAtt                                                                                                                    <  1:1280477/140‑1 (MQ=255)                  cGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGAtt                                                                                                                    <  2:464234/140‑1 (MQ=255)                  cGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGGGTGCGTCAGGGGCGCGCGCAACTGCCCCTCTTAAACAACCGTCGCTTTGCGCCGCCGCCATTATTATGGTTTTTTTTTACTCGGCGCTTGAtt                                                                                                                    >  2:685785/1‑140 (MQ=255)                            gCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTa                                                                                                          <  2:116661/140‑1 (MQ=255)                             ccTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTAc                                                                                                         >  1:998648/1‑140 (MQ=255)                              cTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACa                                                                                                        <  2:530076/140‑1 (MQ=255)                              cTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACa                                                                                                        <  2:1121040/140‑1 (MQ=255)                                           ggCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtg                                                                                           >  2:1050033/1‑140 (MQ=255)                                            gCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATtgtgt                                                                                          >  1:913939/1‑140 (MQ=255)                                               gAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcg                                                                                       <  2:750286/140‑1 (MQ=255)                                               gAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcg                                                                                       <  2:986855/140‑1 (MQ=255)                                                aaGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgc                                                                                      >  2:291976/1‑140 (MQ=255)                                                aaGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgc                                                                                      >  2:1105139/1‑140 (MQ=255)                                                aaGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgc                                                                                      >  2:949803/1‑140 (MQ=255)                                                aaGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcac                                                                                      >  2:1216105/1‑138 (MQ=255)                                                aaGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGACGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgc                                                                                      >  2:1076996/1‑140 (MQ=255)                                                    gACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgcg                                                                                     >  1:717845/1‑137 (MQ=255)                                                    gACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTgcgcg                                                                                     <  2:717845/137‑1 (MQ=255)                                                         ccGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAAt                                                                             <  2:1184750/140‑1 (MQ=255)                                                            aaCGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGAc                                                                          >  2:174610/1‑140 (MQ=255)                                                            aaCGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGAc                                                                          <  2:998648/140‑1 (MQ=255)                                                              cGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCg                                                                        <  2:428593/140‑1 (MQ=255)                                                                       cAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATtc                                                               >  2:1082896/1‑140 (MQ=255)                                                                        aGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATtct                                                              <  1:1082896/140‑1 (MQ=255)                                                                         ggTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATtctc                                                             <  2:720616/140‑1 (MQ=255)                                                                         ggTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATtctc                                                             <  2:1292435/140‑1 (MQ=255)                                                                                 cgcAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGttttt                                                     >  2:661174/1‑140 (MQ=255)                                                                                    aaCTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAg                                                  <  2:1052327/140‑1 (MQ=255)                                                                                              cTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAg                                                  <  2:1205864/130‑1 (MQ=255)                                                                                              cTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAg                                                  >  1:1205864/1‑130 (MQ=255)                                                                                              cTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGcgcg                                             >  1:506893/1‑135 (MQ=255)                                                                                              cTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGcgcg                                             <  2:506893/135‑1 (MQ=255)                                                                                                 aaaCAACCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTg                                     <  1:174610/140‑1 (MQ=255)                                                                                                 aaaCAACCGACGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTg                                     <  1:1076996/140‑1 (MQ=255)                                                                                                  aacaacCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGc                                    <  2:913939/140‑1 (MQ=255)                                                                                                  aacaacCGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGc                                    <  1:949803/140‑1 (MQ=255)                                                                                                       ccGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGtt                                           <  2:840955/128‑1 (MQ=255)                                                                                                       ccGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGtt                                           >  1:840955/1‑128 (MQ=255)                                                                                                       ccGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGtt                               >  2:160970/1‑140 (MQ=255)                                                                                                       ccGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGtt                               <  2:257364/140‑1 (MQ=255)                                                                                                        cGTCGCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTta                              >  1:543059/1‑140 (MQ=255)                                                                                                            gCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATg                          >  2:897811/1‑140 (MQ=255)                                                                                                            gCTTTGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATg                          <  2:858780/140‑1 (MQ=255)                                                                                                               ttGCGCCGCCGCAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCa                       <  2:543059/140‑1 (MQ=255)                                                                                                                  cgccgccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCaaga                    <  1:1050033/140‑1 (MQ=255)                                                                                                                  cgccgccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCaaga                    <  1:1098257/140‑1 (MQ=255)                                                                                                                  cgccgccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCaag                     >  1:101028/1‑139 (MQ=255)                                                                                                                  cgccgccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCaag                     <  2:101028/139‑1 (MQ=255)                                                                                                                      gccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGt                <  2:267822/140‑1 (MQ=255)                                                                                                                      gccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGt                >  1:804953/1‑140 (MQ=255)                                                                                                                       ccgcAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTa               >  2:332868/1‑140 (MQ=255)                                                                                                                          cAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTtat                             <  2:644771/123‑1 (MQ=255)                                                                                                                          cAATTATTATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTtat                             >  1:644771/1‑123 (MQ=255)                                                                                                                            attattATGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTAt          >  1:867006/1‑140 (MQ=255)                                                                                                                             ttattaTGATTTTTTTTTACTCGGCGCTTGATTCGCCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATg         <  1:1191480/140‑1 (MQ=255)                                                                                                                               attatGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATggg       <  1:160970/140‑1 (MQ=255)                                                                                                                               attatGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATggg       <  1:661174/140‑1 (MQ=255)                                                                                                                                ttatGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCACGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGt      <  1:1216105/140‑1 (MQ=255)                                                                                                                                 tatGATTTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTg     >  1:1211808/1‑140 (MQ=255)                                                                                                                                  atgattTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTga    >  2:1191576/1‑140 (MQ=255)                                                                                                                                    gattTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTgaga  <  2:156099/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAAGTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCGTCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATTATGATGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCTGGTTTTTAAGGCGCGTTCTGTTGCCGGTTATATGTCAAGAAGGTATCTATGGGTGAGA  >  NC_000913/658686‑658956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑