Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	726,385	C→A	E218* (GAA→TAA)	kdpB ←	K(+) transporting P‑type ATPase subunit KdpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	726,385	0	C	A	100.0%	117.4 / NA	35	E218* (GAA→TAA)	kdpB	K(+) transporting P‑type ATPase subunit KdpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/19);  total (16/19)

ATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGAGG  >  NC_000913/726249‑726516                                                                                                                                         |                                                                                                                                    aTCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATttagtt                                                                                                                                  >  2:739672/1‑140 (MQ=255) aTCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCACCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATttagtt                                                                                                                                  >  2:350681/1‑140 (MQ=255)   cagacagaCCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATttagttcg                                                                                                                                <  1:118037/140‑1 (MQ=255)   cagacagaCCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATttagttcg                                                                                                                                >  1:40708/1‑140 (MQ=255)   cagacagaCCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGGAAAAAGAAGATAGTCCGGGGAATTAGCAGAAAGGTCAGGGGAATttagttcc                                                                                                                                >  2:941523/1‑139 (MQ=255)     gacagacCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCCGTAAAAAAACGAAAGTCAGGGGAAACAGCAGAATGGTCAGGGCAATTTAGTTTggg                                                                                                                              >  2:911789/1‑139 (MQ=255)          acCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGGAATCAGCAGAAAGGGCAGGGGAATTTAGTTCGGCGtttt                                                                                                                         >  2:892881/1‑140 (MQ=255)                    ccaccaGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGATAGTCAGGGCAATCAGCAGAATGGGCAGGGCAATTTAGTTCGGCGTTTTTCGTCGCtgt                                                                                                               >  2:999310/1‑140 (MQ=255)                    ccaccaGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAAACGATAGTCAGGGCAAACAGCAGAATGGTCAGGGCAATTTAGTTTGGCGTTTTGCGTCGCtgt                                                                                                               >  2:1131354/1‑140 (MQ=255)                     caccaGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCtgtg                                                                                                              <  2:781633/140‑1 (MQ=255)                     caccaGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCtgtg                                                                                                              <  1:739672/140‑1 (MQ=255)                     caccaGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCtgtg                                                                                                              <  1:350681/140‑1 (MQ=255)                         aGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGcc                                                                                                          <  1:941523/140‑1 (MQ=255)                              cGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTcca                                                                                                     <  1:892881/140‑1 (MQ=255)                                 tACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTccacca                                                                                                  <  2:40708/140‑1 (MQ=255)                                 tACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTccacca                                                                                                  <  2:1075121/140‑1 (MQ=255)                                     cTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCtgtg                                                                                                              <  1:51859/124‑1 (MQ=255)                                     cTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCtgtg                                                                                                              >  2:51859/1‑124 (MQ=255)                                        aCTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGat                                                                                           >  1:1054230/1‑140 (MQ=255)                                            cATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGatcatc                                                                                       <  2:681380/140‑1 (MQ=255)                                            cATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGatcatc                                                                                       <  1:236409/140‑1 (MQ=255)                                            cATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGatcatc                                                                                       <  2:315743/140‑1 (MQ=255)                                                aCCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGAt                                                                                   <  1:353875/140‑1 (MQ=255)                                                  cGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATcc                                                                                 >  2:273815/1‑140 (MQ=255)                                                      ccACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGaa                                                                             >  1:894315/1‑140 (MQ=255)                                                         cgcgGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATg                                                                          >  1:791574/1‑140 (MQ=255)                                                              aaaaCGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCg                                                                     <  2:791574/140‑1 (MQ=255)                                                                     ccACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGt                                                              <  1:273815/140‑1 (MQ=255)                                                                     ccACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGt                                                              <  1:528506/140‑1 (MQ=255)                                                                        caGCGTGGCGGTTGCCAGGAAAAAGAAGATAGTCAGGGGAATCAGCAGAATGGTCAGGGGAATTTAGTTTGGCGTTTTGCGTCGCTGTGCGCCTTCCACCCTCGCGGTCATCCGATCCAAAAATGTCTCGCCGGGGGTaa                                                           >  2:528506/1‑140 (MQ=255)                                                                                 ggTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACt                                                  <  2:1287594/140‑1 (MQ=255)                                                                                                    aTAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGt                                   >  2:331977/1‑136 (MQ=255)                                                                                                    aTAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGt                                   <  1:331977/136‑1 (MQ=255)                                                                                                    aTAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCaga                               >  2:1125706/1‑140 (MQ=255)                                                                                                               aaTCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCaga                               >  1:716657/1‑129 (MQ=255)                                                                                                               aaTCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCaga                               <  2:716657/129‑1 (MQ=255)                                                                                                                aTCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGt                   >  1:216076/1‑140 (MQ=255)                                                                                                                 tCAGCAGAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTg                  >  2:1180604/1‑140 (MQ=255)                                                                                                                   agcagAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTgcc                <  1:1125706/140‑1 (MQ=255)                                                                                                                      agAATGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTgccgcc             >  2:437784/1‑140 (MQ=255)                                                                                                                          tGGTCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAATTGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTg         >  2:670938/1‑140 (MQ=255)                                                                                                                             tCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACg      >  2:196505/1‑140 (MQ=255)                                                                                                                             tCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACg      >  2:1067188/1‑140 (MQ=255)                                                                                                                             tCAGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACg      >  1:850052/1‑140 (MQ=255)                                                                                                                               aGGGCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGa    >  2:129721/1‑140 (MQ=255)                                                                                                                                gggCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGAg   >  2:1248748/1‑140 (MQ=255)                                                                                                                                 ggCAATTTAGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGAgg  >  1:495401/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    ATCAGACAGACCAGCAGCGCCACCAGTACCGTTACGCTGACTGCATTACCGCCCCACGCGGAAAACGGCCACAGCGTGGCGGTTGCCAGTAAAAAGACGATAGTCAGGGCAATCAGCAGAATGGTCAGGGCAATTTCGTTCGGCGTTTTGCGTCGCTGTGCGCCTTCCACCATCGCGATCATCCGATCCAGAAATGTCTCGCCGGGGTTAACGCTACACTCAATCACCAGCCAGTCAGAAAGAATACGCGTGCCGCCGGTGACGGAGG  >  NC_000913/726249‑726516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑