Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,287,614 A→G noncoding (9/85 nt) tyrT ← tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,6140AG98.8% 292.3 / ‑4.6 82noncoding (9/85 nt)tyrTtRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (46/35);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

TCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGAACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAA  >  NC_000913/1287477‑1287747
                                                                                                                                           |                                                                                                                                      
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAGGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  1:510826/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CGGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  2:446902/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      <  1:1034521/140‑1 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  1:882186/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  2:1034521/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  2:1118075/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  2:316973/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAc                                                                                                                                      >  2:41041/1‑140 (MQ=255)
tCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGAAc                                                                                                                                      >  1:102505/1‑140 (MQ=255)
  cccGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAccc                                                                                                                                    <  2:200828/140‑1 (MQ=255)
  cccGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGAccc                                                                                                                                    <  2:605395/140‑1 (MQ=255)
       tGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCccacca                                                                                                                               >  2:1152817/1‑140 (MQ=255)
       tGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCccacca                                                                                                                               <  1:595452/140‑1 (MQ=255)
         aGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAGGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑Cg                                                                                                                            <  2:510826/140‑1 (MQ=255)
           tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCccacca                                                                                                                               <  2:982312/136‑1 (MQ=255)
           tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCccacca                                                                                                                               >  1:982312/1‑136 (MQ=255)
           tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑Cggg                                                                                                                          >  2:150650/1‑140 (MQ=255)
           tGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGACGACGG‑CAGATTTACAGT‑CTGCTCTCATTCGCCGCTCGGGGACCCCACCA‑Cggg                                                                                                                          >  2:992249/1‑140 (MQ=21)
                   ttCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCt                                                                                                                  <  2:940596/140‑1 (MQ=255)
                   ttCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCt                                                                                                                  <  1:792669/140‑1 (MQ=255)
                              aCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGcc                                                                                                       <  1:150650/140‑1 (MQ=255)
                              aCCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGcc                                                                                                       <  1:635192/140‑1 (MQ=255)
                               ccGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCt                                                                                                      <  2:84275/140‑1 (MQ=255)
                                  aaGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCt                                                                                                   <  2:259080/140‑1 (MQ=255)
                                    gTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGACGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTcc                                                                                                 >  2:1172602/1‑140 (MQ=255)
                                      tACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCt                                                                                               <  2:1001641/140‑1 (MQ=255)
                                            aTCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCgg                                                                                         >  2:939680/1‑140 (MQ=255)
                                              cGCTGTGGTGAATTACGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGa                                                                                       >  1:836930/1‑140 (MQ=255)
                                                 tgtgGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTccc                                                                                                >  2:869600/1‑128 (MQ=255)
                                                 tgtgGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTccc                                                                                                <  1:869600/128‑1 (MQ=255)
                                                     gtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATc                                                                                           >  1:1201189/1‑129 (MQ=255)
                                                     gtgAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATc                                                                                           <  2:1201189/129‑1 (MQ=255)
                                                        aaTTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgcgc                                                                             <  1:939680/140‑1 (MQ=255)
                                                         gtgaTGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGca                                                                            <  1:999275/137‑1 (MQ=255)
                                                           tATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGcatc                                                                          <  1:992249/140‑1 (MQ=255)
                                                               gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCatat                                                                      >  1:1270921/1‑140 (MQ=255)
                                                               gtggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCatat                                                                      >  1:56383/1‑140 (MQ=255)
                                                                tggtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATc                                                                     <  2:1155280/140‑1 (MQ=255)
                                                                  gtggGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCaa                                                                   >  2:266692/1‑140 (MQ=255)
                                                                    gggggAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAAt                                                                 >  2:280852/1‑140 (MQ=255)
                                                                     ggggAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATg                                                                >  2:416012/1‑140 (MQ=255)
                                                                     ggggAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATg                                                                >  1:1205743/1‑140 (MQ=255)
                                                                       ggAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGAc                                                              >  2:770933/1‑140 (MQ=255)
                                                                         aaGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGAcgc                                                            >  1:1249898/1‑140 (MQ=255)
                                                                          aGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGAcgcg                                                           <  2:882186/140‑1 (MQ=255)
                                                                          aGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGAcgcg                                                           <  2:836930/140‑1 (MQ=255)
                                                                           ggATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACgcgc                                                          <  1:307235/140‑1 (MQ=255)
                                                                             aTTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGcgccg                                                        <  1:93696/140‑1 (MQ=255)
                                                                                cGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGC‑TTTTACTTGCCTGCTCCCTTTTCGGGGAGGCGGGCGCATCATATCAAATGACGGGCCCCTGt                                                    >  1:466370/1‑140 (MQ=21)
                                                                                  gaCCCTCCAAGTCGAAGGCCGCCAGATTT‑CAGTCCTGCT‑CCTTTGGGCGGTCGGGGGCCCCCCCACCGGGGTAATG‑TTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTa                                                   <  2:466370/139‑1 (MQ=255)
                                                                                  aaCCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTa                                                   <  1:560672/140‑1 (MQ=255)
                                                                                   aCCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTaa                                                  >  1:564534/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCt                                                      >  2:646050/1‑133 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCt                                                      <  1:646050/133‑1 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCt                                                      >  2:683547/1‑133 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCt                                                      <  1:683547/133‑1 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:1175217/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  2:592035/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:50938/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:172971/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  2:1165670/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:313300/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:716656/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:826083/1‑140 (MQ=255)
                                                                                      ttCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgt                                               >  1:927784/1‑140 (MQ=255)
                                                                                       tCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGcgccg                                                        >  1:1157456/1‑130 (MQ=255)
                                                                                       tCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGcgccg                                                        <  2:1157456/130‑1 (MQ=255)
                                                                                       tCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgtg                                              >  2:58922/1‑140 (MQ=255)
                                                                                       tCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgtg                                              >  2:1051/1‑140 (MQ=255)
                                                                                       tCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgtg                                              >  1:820230/1‑140 (MQ=255)
                                                                                       tCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGAAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgtg                                              >  2:1215232/1‑140 (MQ=255)
                                                                                         gAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGtt                                            <  2:313300/140‑1 (MQ=255)
                                                                                         gAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGtt                                            <  2:1270921/140‑1 (MQ=255)
                                                                                                              tACAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTg                        <  1:592035/140‑1 (MQ=255)
                                                                                                               aCAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCGCCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTgg                       <  1:41041/140‑1 (MQ=255)
                                                                                                                cAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTgct                      <  2:670870/140‑3 (MQ=255)
                                                                                                                cAGT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGt                      <  2:1175217/140‑1 (MQ=255)
                                                                                                                  gT‑CTGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTg                    >  1:752525/1‑140 (MQ=255)
                                                                                                                      tGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGt                 >  2:407669/1‑140 (MQ=255)
                                                                                                                      tGCTCCCTTTGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGt                 >  1:489235/1‑140 (MQ=255)
                                                                                                                              ttGGCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCa         <  1:58922/140‑1 (MQ=255)
                                                                                                                                ggCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATc       >  1:592324/1‑140 (MQ=255)
                                                                                                                                ggCCGCTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATc       >  2:262118/1‑140 (MQ=255)
                                                                                                                                     cTCGGGGACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTaa  <  1:316973/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGG‑CAGATTTACAGT‑CTGCTCCCTTTGGCCGCTCGGGAACCCCACCA‑CGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAA  >  NC_000913/1287477‑1287747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑