Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,728,301	G→T	G357G (GGC→GGA)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,301	0	G	T	100.0%	276.1 / NA	80	G357G (GGC→GGA)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (51/29);  total (51/29)

GCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAA  >  NC_000913/3728165‑3728435                                                                                                                                         |                                                                                                                                       gCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCa                                                                                                                                     <  1:608010/140‑1 (MQ=255)     gTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGtt                                                                                                                                 >  1:524085/1‑140 (MQ=255)       aCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTtga                                                                                                                               <  2:234957/140‑1 (MQ=255)       aCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTtga                                                                                                                               <  2:794183/140‑1 (MQ=255)           aCTACGCGCCCCGCCCCAAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCGCTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAg                                                                                                                           >  2:672940/1‑140 (MQ=255)               cgcgCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCaa                                                                                                                       >  2:511508/1‑140 (MQ=255)                gcgcCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTtgat                                                                                                                              <  1:1081047/132‑1 (MQ=255)                gcgcCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTtgat                                                                                                                              >  2:1081047/1‑132 (MQ=255)                gcgcCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCaaa                                                                                                                      >  1:1099985/1‑140 (MQ=255)                gcgcCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCaaa                                                                                                                      >  1:833438/1‑140 (MQ=255)                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAATTCCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAAcc                                                                                                                    <  1:479343/140‑1 (MQ=255)                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAAcc                                                                                                                    <  2:833438/140‑1 (MQ=255)                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAAcc                                                                                                                    >  1:560719/1‑140 (MQ=255)                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAAcc                                                                                                                    >  1:72822/1‑140 (MQ=255)                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAAcc                                                                                                                    >  1:828486/1‑140 (MQ=255)                  gccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCACCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAAcc                                                                                                                    >  1:493564/1‑140 (MQ=255)                   ccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCa                                                                                                                   >  2:888871/1‑140 (MQ=255)                   ccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAATGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCa                                                                                                                   <  2:493564/140‑1 (MQ=255)                       gccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGa                                                                                                               >  1:202649/1‑140 (MQ=255)                                   cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                                                                                                   >  2:943154/1‑140 (MQ=255)                                   cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                                                                                                   >  2:278871/1‑140 (MQ=255)                                   cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                                                                                                   >  2:390615/1‑140 (MQ=255)                                   cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCACATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg                                                                                                   >  1:424720/1‑140 (MQ=255)                                    gTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGc                                                                                                  <  1:909245/140‑1 (MQ=255)                                    gTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGc                                                                                                  <  1:495009/140‑1 (MQ=255)                                    gTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGc                                                                                                  <  1:521340/140‑1 (MQ=255)                                         acTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTggg                                                                                             >  2:1018687/1‑140 (MQ=255)                                           tttGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCGAAGAAAACCCCCTTCGCCTGGGGa                                                                                           <  2:1099985/140‑1 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCGGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGatt                                                                                         >  1:1173040/1‑140 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCt                                                                                                <  1:698310/133‑1 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCt                                                                                                >  2:698310/1‑133 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCt                                                                                                <  2:224056/133‑1 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCt                                                                                                >  1:224056/1‑133 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGatt                                                                                         >  2:618706/1‑140 (MQ=255)                                             tGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGatt                                                                                         >  2:50131/1‑140 (MQ=255)                                                  tttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCGGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgt                                                                                    <  2:1173040/140‑1 (MQ=255)                                                   ttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtg                                                                                   <  1:943154/140‑1 (MQ=255)                                                     aaTACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg                                                                                 >  2:753005/1‑140 (MQ=255)                                                         ccGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGt                                                                             >  1:842000/1‑140 (MQ=255)                                                            cAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACg                                                                          <  1:753005/140‑1 (MQ=255)                                                                cATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCg                                                                      <  2:740870/140‑1 (MQ=255)                                                                  tGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGcc                                                                    >  1:226482/1‑140 (MQ=255)                                                                    cACGACATCCATGCCATCTGCCAGCGCATAACCCACGTCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCgg                                                                  >  1:399632/1‑140 (MQ=255)                                                                         cATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGa                                                             >  2:61064/1‑140 (MQ=255)                                                                         cATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGa                                                             >  2:319587/1‑140 (MQ=255)                                                                           tCCATGCCATCTGCCAGCGCATAACCCACGTCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATa                                                           <  2:399632/140‑1 (MQ=255)                                                                               tGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGc                                                       >  1:209657/1‑140 (MQ=255)                                                                                   aTCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaa                                                   >  1:498091/1‑140 (MQ=255)                                                                                   aTCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaa                                                   >  1:996053/1‑140 (MQ=255)                                                                                   aTCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaa                                                   >  1:992676/1‑140 (MQ=255)                                                                                   aTCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaa                                                   >  2:450610/1‑140 (MQ=255)                                                                                    tCTGCCAGCGCATAGCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGATTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaaa                                                  <  1:1081870/140‑1 (MQ=255)                                                                                    tCTGCCAGCGCATAACCCACGCCTTCCAGCGCTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGaaa                                                  <  1:672940/140‑1 (MQ=255)                                                                                     cTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAAc                                                 >  2:87612/1‑140 (MQ=255)                                                                                      tGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAAcc                                                >  2:397611/1‑140 (MQ=255)                                                                                          aGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAAc                                            >  1:495771/1‑140 (MQ=255)                                                                                          aGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAAc                                            >  1:42565/1‑140 (MQ=255)                                                                                            cgcATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTg                                          >  2:163526/1‑140 (MQ=255)                                                                                                 aaCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCg                                     >  1:29776/1‑140 (MQ=255)                                                                                                   cccACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGc                                   <  1:319587/140‑1 (MQ=255)                                                                                                      aCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACt                                <  1:87612/140‑1 (MQ=255)                                                                                                       cGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACtt                               >  1:443461/1‑140 (MQ=255)                                                                                                       cGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACtt                               <  1:278871/140‑1 (MQ=255)                                                                                                       cGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCACCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACtt                               >  2:982713/1‑140 (MQ=255)                                                                                                        gCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACttt                              >  2:1024177/1‑140 (MQ=255)                                                                                                          cTTCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTtca                            <  2:209657/140‑1 (MQ=255)                                                                                                           ttCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTtcat                           >  1:1015634/1‑140 (MQ=255)                                                                                                           ttCCAGCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTtcat                           >  2:798627/1‑140 (MQ=255)                                                                                                                gCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGcc                      <  2:524085/140‑1 (MQ=255)                                                                                                                gCACTGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATCGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGcc                      <  1:888871/140‑1 (MQ=255)                                                                                                                    tGCTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGtt                  >  2:79018/1‑140 (MQ=255)                                                                                                                      cTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGa                >  2:351280/1‑140 (MQ=255)                                                                                                                      cTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGa                >  2:429042/1‑140 (MQ=255)                                                                                                                      cTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGa                >  1:288867/1‑140 (MQ=255)                                                                                                                      cTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGa                >  2:1081292/1‑140 (MQ=255)                                                                                                                      cTCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGa                >  2:586149/1‑140 (MQ=255)                                                                                                                       tCGCGCCAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAg               <  2:560719/140‑1 (MQ=255)                                                                                                                            ccAGTTCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCa          <  1:397611/140‑1 (MQ=255)                                                                                                                                ttCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATTAGCCTGTTGAGCTGCAGCGa      >  2:1088218/1‑140 (MQ=255)                                                                                                                                ttCATTGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGa      >  2:111128/1‑140 (MQ=255)                                                                                                                                    ttGGGTCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTaa  <  2:602479/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGAAACCAAACTGGCTCGGCACTTTCATCAGCCTGTTGAGCTGCAGCGATTAA  >  NC_000913/3728165‑3728435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑