Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,306,804 G→A intergenic (‑50/+168) mqo ← / ← yojI malate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,306,8040GA100.0% 180.4 / NA 51intergenic (‑50/+168)mqo/yojImalate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/34);  total (17/34)

CTCCTCGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATT  >  NC_000913/2306668‑2306932
                                                                                                                                        |                                                                                                                                
ctcctcGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCgcagca                                                                                                                               >  2:444275/1‑140 (MQ=255)
ctcctcGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCgcagca                                                                                                                               >  2:587115/1‑140 (MQ=255)
ctcctcGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCgcagca                                                                                                                               <  1:147418/140‑1 (MQ=255)
  cctcGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATc                                                                                                                             <  2:933089/140‑1 (MQ=255)
  cctcGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATc                                                                                                                             <  1:908226/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  2:1051118/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  1:80140/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  2:412626/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  1:444275/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  1:105814/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  1:512887/140‑1 (MQ=255)
        aCGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  1:587115/140‑1 (MQ=255)
           ctttcgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAac                                                                                                                    <  2:652301/140‑1 (MQ=255)
             ttcgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAacac                                                                                                                  <  1:196308/140‑1 (MQ=255)
             ttcgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAacac                                                                                                                  >  2:626532/1‑140 (MQ=255)
              tcgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTAATGCAGGTGAGATTTGCGCAGCATCAACGGTAACACt                                                                                                                 <  1:233926/140‑1 (MQ=255)
                     tGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACt                                                                                                                 >  1:882819/1‑133 (MQ=255)
                     tGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACt                                                                                                                 <  2:882819/133‑1 (MQ=255)
                      gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       <  1:783089/126‑1 (MQ=255)
                      gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGt                                                                                                                       >  2:783089/1‑126 (MQ=255)
                      gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTaac                                                                                                         <  2:388090/140‑1 (MQ=255)
                      gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTaac                                                                                                         <  1:871468/140‑1 (MQ=255)
                        cgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTaacaa                                                                                                       <  1:845808/140‑1 (MQ=255)
                           cATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACAt                                                                                                    <  1:746738/140‑1 (MQ=255)
                            aTCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACAtt                                                                                                   >  2:874553/1‑140 (MQ=255)
                                        ttAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGttt                                                                                       <  2:1039928/140‑1 (MQ=255)
                                          aaGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTtat                                                                                     <  1:32352/140‑1 (MQ=255)
                                                 aCGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATtat                                                                              >  1:158822/1‑140 (MQ=255)
                                                     ccATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTc                                                                          <  1:874553/140‑1 (MQ=255)
                                                      cATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCt                                                                         <  2:489884/140‑1 (MQ=255)
                                                       aTCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTa                                                                        <  1:434909/140‑1 (MQ=255)
                                                        tCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTaa                                                                       >  2:1148245/1‑140 (MQ=255)
                                                                  cATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAAc                                                             >  1:752466/1‑140 (MQ=255)
                                                                    tGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGaaa                                                              <  2:1005641/137‑1 (MQ=255)
                                                                    tGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGaaa                                                              >  1:1005641/1‑137 (MQ=255)
                                                                       cAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATtat                                                        >  1:988372/1‑140 (MQ=255)
                                                                          tCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAAt                                                     >  2:104286/1‑140 (MQ=255)
                                                                                 tttCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTg                                              <  2:886626/140‑1 (MQ=255)
                                                                                   tCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGat                                            <  1:1051776/140‑1 (MQ=255)
                                                                                   tCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGat                                            <  2:506970/140‑1 (MQ=255)
                                                                                     aTCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGatta                                          <  2:158822/140‑1 (MQ=255)
                                                                                      tCTTGTTAATGCTTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGattaa                                         >  1:291403/1‑140 (MQ=255)
                                                                                          gTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATtat                                     <  1:626532/140‑1 (MQ=255)
                                                                                          gTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATtat                                     <  1:831723/140‑1 (MQ=255)
                                                                                           ttAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATtata                                    <  1:49074/140‑1 (MQ=255)
                                                                                               tGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGt                                <  1:553372/140‑1 (MQ=255)
                                                                                                 ccTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGtta                              >  1:870285/1‑140 (MQ=255)
                                                                                                  cTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGttat                             >  2:1123166/1‑140 (MQ=255)
                                                                                                  cTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGttat                             <  1:1123166/140‑1 (MQ=255)
                                                                                                     aCTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAg                          >  2:352817/1‑140 (MQ=255)
                                                                                                             tCGCTTTATTGCAGGTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAg                  <  2:569049/140‑1 (MQ=255)
                                                                                                                          ggTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCat     >  1:1008255/1‑140 (MQ=255)
                                                                                                                           gTGAGATTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCata    >  2:1108726/1‑140 (MQ=255)
                                                                                                                             gagaTTTGCGCAGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATAtt  >  1:123129/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                
CTCCTCGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATT  >  NC_000913/2306668‑2306932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑