Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,459	0	T	C	25.5%	182.9 / 26.8	98	D625G (GAT→GGT)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (53/20);  new base C (0/25);  total (53/45)
Fisher's exact test for biased strand distribution p-value = 2.33e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTC  >  NC_000913/3401326‑3401597                                                                                                                                      |                                                                                                                                            cAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCtt                                                                                                                                      >  1:1364294/1‑140 (MQ=255)   gACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGc                                                                                                                                    <  1:529048/140‑1 (MQ=255)   gACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGc                                                                                                                                    <  1:382131/140‑1 (MQ=255)   gACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGc                                                                                                                                    <  2:498599/140‑1 (MQ=255)    aCAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGcc                                                                                                                                   >  1:768840/1‑140 (MQ=255)    aCAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGcc                                                                                                                                   >  2:1311820/1‑140 (MQ=255)     gacaagcacTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  <  2:37065/134‑1 (MQ=255)     cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  <  2:734472/140‑1 (MQ=255)     cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  <  2:285680/140‑1 (MQ=255)     aaaGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTAAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTCCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAAT‑CCCCTTGccc                                                                                                                                  <  2:333804/139‑1 (MQ=255)      aaGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  <  1:1198922/139‑1 (MQ=255)      aaGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  >  2:1198922/1‑139 (MQ=255)          acacTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTg                                                                                                                             <  1:797965/140‑1 (MQ=255)           cacTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGt                                                                                                                            >  1:1263585/1‑140 (MQ=255)              tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAac                                                                                                                         >  1:864042/1‑140 (MQ=255)              tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAac                                                                                                                         >  2:677045/1‑140 (MQ=255)              tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAac                                                                                                                         >  2:1231485/1‑140 (MQ=255)              tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAac                                                                                                                         >  1:489215/1‑140 (MQ=255)                ccATGAGACGCAGCGCGCATTATTCTACGTAAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTGTCAAGTGGCTGGGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAacac                                                                                                                       <  2:1470300/140‑1 (MQ=255)                  aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  <  2:1145281/127‑1 (MQ=255)                  aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGccc                                                                                                                                  >  1:1145281/1‑127 (MQ=255)                   tGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACcgc                                                                                                                    >  2:1111919/1‑140 (MQ=255)                    gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   >  2:257306/1‑140 (MQ=255)                    gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  1:257306/140‑1 (MQ=255)                    gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  2:1234499/140‑1 (MQ=255)                    gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  2:327713/140‑1 (MQ=255)                    gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACcgcg                                                                                                                   <  2:1263585/140‑1 (MQ=255)                        cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                               >  2:2555/1‑140 (MQ=255)                          cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                             >  1:1565711/1‑140 (MQ=255)                          cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                             >  1:1834301/1‑140 (MQ=255)                          cAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                             >  2:1459466/1‑140 (MQ=255)                            gcgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCa                                                                                                           >  1:1377274/1‑140 (MQ=255)                             cgcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAg                                                                                                          >  1:1703449/1‑140 (MQ=255)                              gcgcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAgg                                                                                                         <  2:93159/140‑1 (MQ=255)                              cagcATTATTCTACGTAAAAACGGATTAAACGGCAGGTTAACCCGAGTATCTTTGTAAATTTTTTTTCCCGTTAGTATCAAGTGGTTGTGAGGCCGCAAAAAACC‑CCCCTCGCCCGCCTGTAACACCGCGCTGAATCAgg                                                                                                         <  2:1320818/138‑1 (MQ=255)                                gcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGt                                                                                                       <  1:1039670/140‑1 (MQ=255)                                gcATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGt                                                                                                       <  1:431559/140‑1 (MQ=255)                                      ttCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAGTCAGGGTCTGCCa                                                                                                 <  1:1245339/140‑1 (MQ=255)                                       tCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCAc                                                                                                >  1:914402/1‑140 (MQ=255)                                          aCGTGAAAACGGATTAAACGGCAGGTTAAACCGGGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCg                                                                                             >  2:1384582/1‑140 (MQ=255)                                            gTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTcctg                                                                                           >  1:1320818/1‑137 (MQ=255)                                            gTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAAAACCGCGCTGAATCAGGGTCTGCCACTCGCt                                                                                           >  1:1649503/1‑140 (MQ=255)                                                             ggCGGGTTACACCAAGTTTTTTTGTAAATTTTTTTTCCTGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAAC‑CCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                          <  2:961205/140‑1 (MQ=255)                                                             ggCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                          <  2:489215/140‑1 (MQ=255)                                                                aGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTgg                                                                       >  1:183793/1‑140 (MQ=255)                                                                  gTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                                     <  1:996396/140‑1 (MQ=255)                                                                      aaCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTaaa                                                                 >  1:1389002/1‑140 (MQ=255)                                                                       aCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAAc                                                                >  1:1513326/1‑140 (MQ=255)                                                                            gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                           >  2:668698/1‑140 (MQ=255)                                                                             tttCTTTGTAAATTTTTTTTCACTTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGt                                                          <  1:164385/138‑1 (MQ=255)                                                                             tATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGt                                                          >  1:1457605/1‑140 (MQ=255)                                                                             tATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGt                                                          <  1:668698/140‑1 (MQ=255)                                                                               tCTTTGTAAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGc                                                        <  2:641529/140‑1 (MQ=255)                                                                                ttttGTAAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTCCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCt                                                       <  2:864042/139‑1 (MQ=255)                                                                                     tgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                                  >  2:1506044/1‑140 (MQ=255)                                                                                      aaaTTTTTTTTCTCTTTATTATCAAGGGGCTGTGAGGAGGCAAAAAAAC‑CCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTccc                                                 <  2:411179/139‑1 (MQ=255)                                                                                       aaTATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCg                                                >  1:411179/1‑140 (MQ=255)                                                                                         tatTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGa                                              >  2:705436/1‑140 (MQ=255)                                                                                          tttttttCACGTTAGTATAAGGTGGCTGTGAGGACGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAg                                             <  2:914402/139‑1 (MQ=255)                                                                                           ttttttCAGGTTAGTATAAAGTGGCTGTGGGGACGCAAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                            <  1:677045/140‑1 (MQ=255)                                                                                              tttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAgg                                         >  2:1017083/1‑140 (MQ=255)                                                                                                ccACGTTAGTATCAGGTGGTTGTGGGGCCGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCt                                       <  2:183793/139‑1 (MQ=255)                                                                                                     ttAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccac                                  >  2:1113222/1‑140 (MQ=255)                                                                                                      tAGTATCAAGTGGTTGTGGGGACGGGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  2:1190593/140‑1 (MQ=255)                                                                                                      tAGTATCAAGTGGTTGTAAGGCCG‑GAAAAAATCCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  1:567720/140‑1 (MQ=255)                                                                                                      tAGTATCAAGTGGCTGTGAGGCCGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  1:1410366/140‑1 (MQ=255)                                                                                                      tAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 >  2:1135731/1‑140 (MQ=255)                                                                                                      tAGTATCAAGTGGCTGGGAGGACGCAAAAAACC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  2:768840/140‑1 (MQ=255)                                                                                                      gcgTATCAAGTGGCGGAGGGAACGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacc                                 <  2:1140337/138‑1 (MQ=255)                                                                                                       aGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacca                                >  1:1067746/1‑140 (MQ=255)                                                                                                           agaaGTGGCTGTGAGGAGGCAAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCt                            <  2:130422/138‑1 (MQ=255)                                                                                                             aaGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCttt                          <  1:1135731/140‑1 (MQ=255)                                                                                                             aaGTGGCTGTGAGGACGCGAAAAAAC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCttt                          <  1:1345789/140‑1 (MQ=255)                                                                                                              aGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCTGAGCAGGCTTCCACCAGGCTTTg                         >  2:1117951/1‑140 (MQ=255)                                                                                                              aGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                         >  1:1190593/1‑140 (MQ=255)                                                                                                                tGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                       >  2:1832004/1‑140 (MQ=255)                                                                                                                tGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                       >  1:825520/1‑140 (MQ=255)                                                                                                                tGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa                       >  2:644548/1‑140 (MQ=255)                                                                                                                 ggCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAc                      >  2:235582/1‑140 (MQ=255)                                                                                                                  gCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:573257/1‑140 (MQ=255)                                                                                                                  gCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:1646417/1‑140 (MQ=255)                                                                                                                  gCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:1140337/1‑140 (MQ=255)                                                                                                                  gCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc                     >  1:1757159/1‑140 (MQ=255)                                                                                                                   cTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                    >  2:1874435/1‑140 (MQ=255)                                                                                                                   cTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca                    >  2:817672/1‑140 (MQ=255)                                                                                                                     gtgAGGCCGCGAAAAACC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc                  <  2:339239/140‑1 (MQ=255)                                                                                                                     gtgAGGACGCAAAAAATC‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc                  <  2:1457605/140‑1 (MQ=255)                                                                                                                       gAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagcag                <  1:1459466/140‑1 (MQ=255)                                                                                                                        aGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagcact               >  1:130422/1‑138 (MQ=255)                                                                                                                        aGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCagcagc               >  1:1487842/1‑140 (MQ=255)                                                                                                                            cgcgAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGTCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt           >  1:646417/1‑140 (MQ=255)                                                                                                                            cgcgAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt           >  1:501165/1‑140 (MQ=255)                                                                                                                            cgcgAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt           >  2:1695866/1‑140 (MQ=255)                                                                                                                            cgcgAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt           >  2:1712559/1‑140 (MQ=255)                                                                                                                              cgAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTtc         >  2:811819/1‑140 (MQ=255)                                                                                                                                    aaT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGtt   <  1:1017083/140‑1 (MQ=255)                                                                                                                                    aaT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGtt   <  1:817672/140‑1 (MQ=255)                                                                                                                                     aT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTc  <  1:1712559/140‑1 (MQ=255)                                                                                                                                     aT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTc  <  2:1067746/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                            CAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAAT‑CCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTTCTCCGTTC  >  NC_000913/3401326‑3401597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑