Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,263,236	C→A	W87L (TGG→TTG)	fruB ←	fructose‑specific PTS multiphosphoryl transfer protein FruB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,263,236	0	C	A	100.0%	195.6 / NA	56	W87L (TGG→TTG)	fruB	fructose‑specific PTS multiphosphoryl transfer protein FruB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/29);  total (27/29)

TGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGC  >  NC_000913/2263103‑2263363                                                                                                                                      |                                                                                                                                tgttgtTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGa                                                                                                                           <  1:1584461/140‑1 (MQ=255) tgttgtTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGa                                                                                                                           >  2:1100932/1‑140 (MQ=255)    tgttgCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGc                                                                                                                        <  2:1112647/140‑1 (MQ=255)     gttgCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGcc                                                                                                                       >  2:1794283/1‑140 (MQ=255)     gttgCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGcc                                                                                                                       >  1:1672974/1‑140 (MQ=255)            aCTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCggg                                                                                                                <  1:1538222/140‑1 (MQ=255)             cTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCggg                                                                                                                >  2:166956/1‑139 (MQ=255)             cTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCggg                                                                                                                <  1:166956/139‑1 (MQ=255)              ttCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATTCCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGaa                                                                                                              <  2:159592/140‑1 (MQ=255)              ttCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGaa                                                                                                              <  2:1060361/140‑1 (MQ=255)                      ttCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGc                                                                                                                        >  2:514124/1‑122 (MQ=255)                      ttCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGc                                                                                                                        <  1:514124/122‑1 (MQ=255)                      ttCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATa                                                                                                      >  1:1199864/1‑140 (MQ=255)                              cGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACg                                                                                              >  1:923962/1‑140 (MQ=255)                               ggAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGc                                                                                             <  2:808788/140‑1 (MQ=255)                                         cTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAg                                                                                   >  1:873898/1‑140 (MQ=255)                                            aGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCAc                                                                                <  1:1794283/140‑1 (MQ=255)                                                 gTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGAt                                                                           <  1:712634/140‑1 (MQ=255)                                                 gTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGAt                                                                           <  1:177151/140‑1 (MQ=255)                                                   gggTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATcg                                                                         <  1:1839082/140‑1 (MQ=255)                                                   ggatCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATcg                                                                         <  1:702857/137‑1 (MQ=255)                                                      tCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTCAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCgg                                                                      >  1:1764156/1‑140 (MQ=255)                                                        aGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGgt                                                                    >  2:1124816/1‑140 (MQ=255)                                                        aGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGgt                                                                    >  2:1769440/1‑140 (MQ=255)                                                        aGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGgt                                                                    >  1:91733/1‑140 (MQ=255)                                                        aGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGgt                                                                    <  2:1672974/140‑1 (MQ=255)                                                        aGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGgt                                                                    >  1:1364417/1‑140 (MQ=255)                                                          cTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGgtgt                                                                  <  1:697281/140‑1 (MQ=255)                                                             gCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCgg                                                               <  2:1582001/140‑1 (MQ=255)                                                               gTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTa                                                             <  1:1823655/140‑1 (MQ=255)                                                                  gCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTg                                                          <  2:1843854/140‑1 (MQ=255)                                                                  gCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTCAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTg                                                          <  2:1764156/140‑1 (MQ=255)                                                                     ggCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCg                                                       <  1:907063/140‑1 (MQ=255)                                                                      gCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCgt                                                      <  2:923962/140‑1 (MQ=255)                                                                       cccAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCgtg                                                     <  2:91733/140‑1 (MQ=255)                                                                        ccAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCgtgt                                                    >  2:384361/1‑140 (MQ=255)                                                                          aGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTgg                                                  <  2:1562264/140‑1 (MQ=255)                                                                           gATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGa                                                 <  1:90054/140‑1 (MQ=255)                                                                           gATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGa                                                 >  2:802576/1‑140 (MQ=255)                                                                             tGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAAt                                               <  2:1408889/140‑1 (MQ=255)                                                                                 cATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCa                                           >  2:702157/1‑140 (MQ=255)                                                                                  aTCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGa                                                 <  1:28770/133‑1 (MQ=255)                                                                                  aTCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGa                                                 >  2:28770/1‑133 (MQ=255)                                                                                      gAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATAcc                                      >  1:18105/1‑140 (MQ=255)                                                                                      gAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATAcc                                      >  1:1328040/1‑140 (MQ=255)                                                                                                       gATTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTg                     >  2:1012726/1‑140 (MQ=255)                                                                                                        aTTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGa                    >  2:1716989/1‑140 (MQ=255)                                                                                                        aTTGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGa                    >  2:456898/1‑140 (MQ=255)                                                                                                          tGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAgg                            <  1:268628/130‑1 (MQ=255)                                                                                                          tGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAgg                            >  2:268628/1‑130 (MQ=255)                                                                                                          tGCCACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAgg                  >  2:28103/1‑140 (MQ=255)                                                                                                             cACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGttt               >  1:578429/1‑140 (MQ=255)                                                                                                             cACGTACGCTACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGttt               >  2:1652524/1‑140 (MQ=255)                                                                                                                      tACCTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTcgc      >  1:1195866/1‑140 (MQ=255)                                                                                                                        ccTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTcgcgc    <  2:8460/140‑1 (MQ=255)                                                                                                                         cTGACCGTCACCCAAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGT‑GAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCgcgcgc  <  1:384361/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATGTTCATCCGAGCTGGCAGCAATACCGATTGCCACGTACGCTACCTGACCGTCACCCCAGGTGACGCCTTCCGGGAACTGAAATACCTGAACGCCGGTTTTCAGCACCTGATCGCGGGTGTCGGTAGTGCCGTGTGGAATAGCAATACCATTGCCGAGGAACGTTGAGGTTTGCTGTTCGCGCGC  >  NC_000913/2263103‑2263363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑