Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	673,452	C→A	P444P (CCG→CCT)	leuS ←	leucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	673,452	0	C	A	100.0%	258.0 / NA	75	P444P (CCG→CCT)	leuS	leucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/38);  total (37/38)

TGTCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTCGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGA  >  NC_000913/673319‑673588                                                                                                                                      |                                                                                                                                         tgtCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCa                                                                                                                                      <  1:618660/138‑1 (MQ=255) tgtCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCa                                                                                                                                      >  2:618660/1‑138 (MQ=255) tgtCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCAtt                                                                                                                                    >  2:1648179/1‑140 (MQ=255)  gtCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTa                                                                                                                                   <  2:1872452/140‑1 (MQ=255)   tCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTAc                                                                                                                                  >  1:718999/1‑140 (MQ=255)        ttCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTAc                                                                                                                             >  2:434868/1‑140 (MQ=255)          cACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAgg                                                                                                                                        >  2:999555/1‑127 (MQ=255)                    tGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCg                                                                                                                 <  2:619069/140‑1 (MQ=255)                       cATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCa                                                                                                              <  1:53599/140‑1 (MQ=255)                       cATACCGTTAACGGTAGGTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCa                                                                                                              <  2:1566691/140‑1 (MQ=255)                           ccGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCAt                                                                                                          >  1:1522529/1‑140 (MQ=255)                            cGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATc                                                                                                         <  1:1540867/140‑1 (MQ=255)                            cGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATc                                                                                                         >  2:1526052/1‑140 (MQ=255)                             gTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCg                                                                                                        >  2:1181889/1‑140 (MQ=255)                                    gTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCgg                                                                                                 <  2:1473446/140‑1 (MQ=255)                                    gTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCgg                                                                                                 <  1:1526052/140‑1 (MQ=255)                                      aGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcg                                                                                               <  2:159602/140‑1 (MQ=255)                                      aGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcg                                                                                               <  2:1391790/140‑1 (MQ=255)                                      aGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcg                                                                                               <  2:1250816/140‑1 (MQ=255)                                      aGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcg                                                                                               <  1:434868/140‑1 (MQ=255)                                      aGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcg                                                                                               <  1:160979/140‑1 (MQ=255)                                      aGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcg                                                                                               <  2:718999/140‑1 (MQ=255)                                         tttCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcgcgc                                                                                            <  2:1315557/140‑1 (MQ=255)                                         tttCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcgcgc                                                                                            <  2:1006545/140‑1 (MQ=255)                                         tttCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGacgcgc                                                                                            <  2:1754183/140‑1 (MQ=255)                                          ttCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGcc                                                                                           >  1:1114947/1‑140 (MQ=255)                                             gCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCa                                                                                        <  1:285186/140‑1 (MQ=255)                                                  cTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAAc                                                                                   >  1:826374/1‑140 (MQ=255)                                                   tCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcgcgc                                                                                            >  2:317433/1‑130 (MQ=255)                                                   tCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGgcgcgc                                                                                            <  1:317433/130‑1 (MQ=255)                                                   tCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACg                                                                                  <  2:826374/140‑1 (MQ=255)                                                    ccGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCTCCATCGGAATCGGCGCGCCCCAGTAACGc                                                                                 <  1:735182/140‑1 (MQ=255)                                                         tCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGACAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACg                                                                            <  2:355997/140‑1 (MQ=255)                                                            gCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGa                                                                         <  2:192955/140‑1 (MQ=255)                                                            gCTTTAATCGGGCTGGTAATGCCGTCAATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGa                                                                         <  1:55814/140‑1 (MQ=255)                                                             cTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGaa                                                                        >  2:681386/1‑140 (MQ=255)                                                             cTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGaa                                                                        >  1:925333/1‑140 (MQ=255)                                                             cTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGaa                                                                        >  2:900361/1‑140 (MQ=255)                                                              tttAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGaaa                                                                       <  2:1885971/140‑1 (MQ=255)                                                                 aaTCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAacac                                                                    >  1:1353834/1‑140 (MQ=255)                                                                       gCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGt                                                              >  2:103479/1‑140 (MQ=255)                                                                       gCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGt                                                              >  1:1668421/1‑140 (MQ=255)                                                                       gCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCAGCAGCTGGTCGTCCGGGGTAGGCATTACGTTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGt                                                              <  1:1755042/140‑1 (MQ=255)                                                                            tAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTAACGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCCGTCgcgc                                                         >  2:522711/1‑140 (MQ=255)                                                                             aaTGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCa                                                        <  2:1114947/140‑1 (MQ=255)                                                                                 ccGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCg                                                    >  1:1362208/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCTAGTCGCGCAGGCGGTAg                                                >  1:278406/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAg                                                >  2:331333/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAg                                                >  1:191613/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAg                                                >  2:150643/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAg                                                >  1:1300577/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAg                                                >  2:429000/1‑140 (MQ=255)                                                                                     ccATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAg                                                >  2:1386640/1‑140 (MQ=255)                                                                                       aTTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGtt                                              <  2:1387530/140‑1 (MQ=255)                                                                                       aTTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGtt                                              <  1:1131108/140‑1 (MQ=255)                                                                                           ccACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGCAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACt                                          >  2:1699865/1‑140 (MQ=255)                                                                                              caTCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTa                                       >  1:971009/1‑140 (MQ=255)                                                                                                   tCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTc                                  >  1:933294/1‑140 (MQ=255)                                                                                                     cGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGc                                >  2:1400695/1‑140 (MQ=255)                                                                                                      ggCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGcc                               <  2:1036456/140‑1 (MQ=255)                                                                                                      ggCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGcc                               >  1:1243884/1‑140 (MQ=255)                                                                                                       gCAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCa                              <  2:1362208/140‑1 (MQ=255)                                                                                                        cAGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCaa                             <  2:925333/140‑1 (MQ=255)                                                                                                         aGGATCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAAc                            <  1:331333/140‑1 (MQ=255)                                                                                                            aTCACCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGcc                         >  2:1571147/1‑140 (MQ=255)                                                                                                               aCCGGCAGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCAt                      <  1:696056/140‑1 (MQ=255)                                                                                                                     aGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagt                <  1:1089378/140‑1 (MQ=255)                                                                                                                     aGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagt                <  2:971009/140‑1 (MQ=255)                                                                                                                     aGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagt                <  1:681386/140‑1 (MQ=255)                                                                                                                     aGCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCCCCAACGCCCATCGcagt                >  1:1229132/1‑140 (MQ=255)                                                                                                                      gCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagtc               >  1:1703880/1‑140 (MQ=255)                                                                                                                      gCTGGTCGTCCGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCACCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagtc               >  1:617023/1‑140 (MQ=255)                                                                                                                      gCTGGTCGTCCGGGGTAGGCACTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagt                >  2:498134/1‑139 (MQ=255)                                                                                                                      gCTGGTCGTCCGGGGTAGGCACTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGcagt                <  1:498134/139‑1 (MQ=255)                                                                                                                               ccGGGGTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCg      >  2:1352400/1‑140 (MQ=255)                                                                                                                                   ggTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCTAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGa  <  2:278406/140‑1 (MQ=255)                                                                                                                                   ggTAGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGa  <  1:609211/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TGTCGGTTTCACGCAGTGCTGGCATACCGTTAACGGTAGTTTTCGCCCACTCCGGATCTGCTTTAATCGGGCTGGTAATGCCGTCCATTACCACATCTTCCGGCAGGATCACCGGCAGCTGGTCGTCCGGGGTCGGCATTACGGTACCGTCTTCCAGCGTCACCATCGGAATCGGCGCGCCCCAGTAACGCTGACGGGAAACACCCCAGTCGCGCAGGCGGTAGTTCACTTTACGCTCGCCAACGCCCATCGCAGTCAGTTTATCGGCGA  >  NC_000913/673319‑673588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑