Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,787	C→A	S36* (TCG→TAG)	ycgL →	PF05166 family protein YcgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,787	0	C	A	100.0%	304.8 / NA	87	S36* (TCG→TAG)	ycgL	PF05166 family protein YcgL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/42);  total (45/42)

TTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTT  >  NC_000913/1227651‑1227918                                                                                                                                         |                                                                                                                                    ttGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTagcg                                                                                                                                  <  2:828100/140‑1 (MQ=255) ttGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTagcg                                                                                                                                  <  1:1514450/140‑1 (MQ=255)   gtgGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCgtg                                                                                                                                >  1:973913/1‑140 (MQ=255)         cgGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTg                                                                                                                          >  1:1299415/1‑140 (MQ=255)           ggTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAg                                                                                                                        <  1:1223747/140‑1 (MQ=255)              ttCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGaa                                                                                                                     <  2:1299415/140‑1 (MQ=255)                      tGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGaa                                                                                                             <  2:1030742/140‑1 (MQ=255)                              tATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTg                                                                                                     >  2:947359/1‑140 (MQ=255)                               aTGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTgg                                                                                                    >  2:1510937/1‑140 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   <  2:973913/140‑1 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   >  1:767327/1‑140 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   >  1:46253/1‑140 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   <  2:131118/140‑1 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   >  2:1366353/1‑140 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   >  1:426017/1‑140 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   >  1:216076/1‑140 (MQ=255)                                tGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGt                                                                                                   <  1:1605865/140‑1 (MQ=255)                                       aaccggCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTc                                                                                            >  2:426339/5‑140 (MQ=255)                                           ggCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGtt                                                                                        <  1:1655389/140‑1 (MQ=255)                                                 ttAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCgat                                                                                  >  2:41854/1‑140 (MQ=255)                                                   aaaGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCgatga                                                                                <  1:1252515/140‑1 (MQ=255)                                                     agagTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGAtt                                                                              <  1:884249/140‑1 (MQ=255)                                                     agagTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGAtt                                                                              <  1:736113/140‑1 (MQ=255)                                                         tAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGc                                                                          >  2:1849249/1‑140 (MQ=255)                                                         tAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGc                                                                          >  2:1872166/1‑140 (MQ=255)                                                          aaaaGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGcc                                                                         >  2:1520152/1‑140 (MQ=255)                                                           aaaGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCg                                                                        >  1:1070399/1‑140 (MQ=255)                                                           aaaGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCg                                                                        >  2:1172238/1‑140 (MQ=255)                                                                 tGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAt                                                                  >  1:1158076/1‑140 (MQ=255)                                                                 tGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAt                                                                  >  2:509062/1‑140 (MQ=255)                                                                 tGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAt                                                                  >  2:330311/1‑140 (MQ=255)                                                                   ttttGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATgg                                                                <  2:822372/140‑1 (MQ=255)                                                                      tgtgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCg                                                                        >  2:736208/1‑129 (MQ=255)                                                                      tgtgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCg                                                                        <  1:736208/129‑1 (MQ=255)                                                                        tgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGga                                                           <  2:216076/140‑1 (MQ=255)                                                                        tgtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGga                                                           >  1:198914/1‑140 (MQ=255)                                                                         gtgATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaa                                                          >  2:1635493/1‑140 (MQ=255)                                                                           gATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAGGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGgaaga                                                        >  2:1625215/1‑140 (MQ=255)                                                                            aTTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGcc                                                                         >  1:399286/1‑122 (MQ=255)                                                                            aTTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGcc                                                                         <  2:399286/122‑1 (MQ=255)                                                                             tttATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGaaa                                                      <  2:1158076/140‑1 (MQ=255)                                                                             tttATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGaaa                                                      <  2:1730788/140‑1 (MQ=255)                                                                                   gAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAGGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc                                                <  1:1625215/140‑1 (MQ=255)                                                                                   gAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTc                                                <  2:426017/140‑1 (MQ=255)                                                                                    aaGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCa                                               >  2:210292/1‑140 (MQ=255)                                                                                       cagcaAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATg                                            >  2:894600/1‑140 (MQ=255)                                                                                        agcaAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGc                                           <  2:198914/140‑1 (MQ=255)                                                                                        agcaAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGc                                           <  2:701492/140‑1 (MQ=255)                                                                                          caAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCg                                         >  1:1778161/1‑140 (MQ=255)                                                                                           aaGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGa                                        <  1:1849249/140‑1 (MQ=255)                                                                                            aGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGat                                       <  2:1632268/140‑1 (MQ=255)                                                                                                 gACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGa                                  <  2:1070399/140‑1 (MQ=255)                                                                                                  aCCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaa                                 <  2:259462/140‑1 (MQ=255)                                                                                                  aCCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaa                                 <  1:1872166/140‑1 (MQ=255)                                                                                                  aCCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaa                                 >  1:783664/1‑140 (MQ=255)                                                                                                   ccAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGaaa                                <  1:928196/140‑1 (MQ=255)                                                                                                       aCCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGc                                           >  1:251787/1‑125 (MQ=255)                                                                                                       aCCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGc                                           <  2:251787/125‑1 (MQ=255)                                                                                                       aCCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGt                            >  1:1154462/1‑140 (MQ=255)                                                                                                       aCCTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGt                            >  2:297698/1‑140 (MQ=255)                                                                                                       aCCTACTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGt                            >  2:1662574/1‑140 (MQ=255)                                                                                                        ccTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGtt                           >  2:760725/1‑140 (MQ=255)                                                                                                         cTATTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTa                          <  1:760725/140‑1 (MQ=255)                                                                                                           aTTTATATGTCGAAAAAAAAGACGATTTTTATCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTaaa                        <  2:83282/140‑1 (MQ=255)                                                                                                           aTTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTaaa                        <  1:426339/140‑1 (MQ=255)                                                                                                           aTTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTaaa                        <  2:767327/140‑1 (MQ=255)                                                                                                           aTTTATATGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTaaa                        <  2:1171548/140‑1 (MQ=255)                                                                                                                 atGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCa                  >  2:907350/1‑140 (MQ=255)                                                                                                                 atGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCa                  <  2:242759/140‑1 (MQ=255)                                                                                                                  tGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCAt                 >  1:454238/1‑140 (MQ=255)                                                                                                                  tGTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAG‑TAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCAtt                >  2:539465/1‑140 (MQ=255)                                                                                                                   gTCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCAtt                >  2:299853/1‑140 (MQ=255)                                                                                                                    tCGAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTa               >  2:1840187/1‑140 (MQ=255)                                                                                                                     cGAAAAAAAAGTCGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTaa              >  1:1445531/1‑140 (MQ=255)                                                                                                                      gAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAAc             >  1:1201073/1‑140 (MQ=255)                                                                                                                      gAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAAc             >  1:1580646/1‑140 (MQ=255)                                                                                                                      gAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAAc             >  2:94338/1‑140 (MQ=255)                                                                                                                      gAAAAAAAAGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAAc             >  1:1228327/1‑140 (MQ=255)                                                                                                                       aaaaaaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAAcc            >  1:1703117/1‑140 (MQ=255)                                                                                                                        aaaaaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCg           <  1:438925/140‑1 (MQ=255)                                                                                                                          aaaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAg         <  1:935465/140‑1 (MQ=255)                                                                                                                          aaaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAg         <  1:947359/140‑1 (MQ=255)                                                                                                                          aaaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAg         <  1:173046/140‑1 (MQ=255)                                                                                                                          aaaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAg         <  1:1662574/140‑1 (MQ=255)                                                                                                                           aaaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGc        <  1:1366353/140‑1 (MQ=255)                                                                                                                            aaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCa       <  2:1662089/140‑1 (MQ=255)                                                                                                                            aaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCa       <  2:1438680/140‑1 (MQ=255)                                                                                                                            aaaGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCa       <  1:1544507/140‑1 (MQ=255)                                                                                                                              aGACGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAg     >  1:1323022/1‑140 (MQ=255)                                                                                                                                 cGATTTTTAGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGGAAAGGTTAAACAGGCATTAACCGAGCAAGGtt  <  1:1635493/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TTGTGGCGCGGGTTTCATTGATGTTAGCTTATGCCAAAACCTGGCATCTTAAAGAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGATGGGCGGAAGAAACTGGTCAATGCCGATATTGAAAAGGTTAAACAGGCATTAACCGAGCAAGGTT  >  NC_000913/1227651‑1227918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑