Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,159,783	G→T	T466T (ACG→ACT)	mdtC →	multidrug efflux pump RND permease subunit MdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,159,783	0	G	T	100.0%	321.7 / NA	91	T466T (ACG→ACT)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/55);  total (36/55)

TTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACGCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCC  >  NC_000913/2159646‑2159916                                                                                                                                          |                                                                                                                                      ttGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGActct                                                                                                                                     >  1:1841214/1‑140 (MQ=255) ttGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGActct                                                                                                                                     >  1:1344336/1‑140 (MQ=255)   gCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCttt                                                                                                                                   >  2:119098/1‑140 (MQ=255)      aGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTg                                                                                                                                >  1:974422/1‑140 (MQ=255)       gCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGt                                                                                                                               >  2:1881574/1‑140 (MQ=255)       gCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGt                                                                                                                               <  1:1704604/140‑1 (MQ=255)         cGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCg                                                                                                                             >  2:1121321/1‑140 (MQ=255)          gCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGc                                                                                                                            <  1:1881574/140‑1 (MQ=255)          gCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGc                                                                                                                            <  2:711368/140‑1 (MQ=255)           cACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGcc                                                                                                                           <  2:689293/140‑1 (MQ=255)           cACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGcc                                                                                                                           <  1:1579713/140‑1 (MQ=255)           cACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGcc                                                                                                                           <  2:1344336/140‑1 (MQ=255)           cACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGcc                                                                                                                           >  2:1010220/1‑140 (MQ=255)            ccTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTGGAGGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  2:368866/139‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCTGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  1:646093/140‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  1:1500711/140‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  2:1841214/140‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  1:811084/140‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  1:1706309/140‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  1:1556052/140‑1 (MQ=255)            aCTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCa                                                                                                                          <  1:922825/140‑1 (MQ=255)             cTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCAt                                                                                                                         <  1:176535/140‑1 (MQ=255)             cTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCAt                                                                                                                         <  2:677571/140‑1 (MQ=255)             cTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCAt                                                                                                                         <  1:567787/140‑1 (MQ=255)                   catttatgcgAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGACGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGtat                                                                                                                   <  2:315019/133‑1 (MQ=255)                    gTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGtata                                                                                                                  <  2:80683/140‑1 (MQ=255)                     tACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTatat                                                                                                                 <  1:1121321/140‑1 (MQ=255)                       cTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCg                                                                                                               <  1:266351/140‑1 (MQ=255)                       cTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCg                                                                                                               <  1:1029765/140‑1 (MQ=255)                        tCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGt                                                                                                                               >  2:656034/1‑123 (MQ=255)                        tCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGt                                                                                                                               <  1:656034/123‑1 (MQ=255)                        tCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTc                                                                                                                              <  1:695638/124‑1 (MQ=255)                        tCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTc                                                                                                                              >  2:695638/1‑124 (MQ=255)                        tCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGt                                                                                                              >  2:1545715/1‑140 (MQ=255)                        tCGCGAAGACGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGATCCTGTCGCCGGTGTTGATAGGCGGAGTGCAGGGCCGACTGTTACGCGGATTTGCCGTGACTATTTCTGTCGGCATTGGCATATCGt                                                                                                              >  2:191072/1‑140 (MQ=255)                             aaGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTg                                                                                                         <  2:1404278/140‑1 (MQ=255)                              aGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTgg                                                                                                        >  2:1070103/1‑140 (MQ=255)                               gTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  2:142926/1‑140 (MQ=255)                               gTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  2:349509/1‑140 (MQ=255)                               gTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  2:601396/1‑140 (MQ=255)                               gTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  2:1535306/1‑140 (MQ=255)                               gTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  1:1369154/1‑140 (MQ=255)                               gTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       <  1:349509/140‑1 (MQ=255)                                tCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGtt                                                                                                      <  2:246340/140‑1 (MQ=255)                                           gTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACAtt                                                                                           <  2:1005057/140‑1 (MQ=255)                                           gTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACAtt                                                                                           <  1:912223/140‑1 (MQ=255)                                           gTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCCGGTTTCTCTGACAtt                                                                                           <  2:1572004/140‑1 (MQ=255)                                            tGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       <  1:430745/127‑1 (MQ=255)                                            tGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  2:1511551/1‑127 (MQ=255)                                            tGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       >  2:430745/1‑127 (MQ=255)                                            tGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGt                                                                                                       <  1:1511551/127‑1 (MQ=255)                                             gCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTaa                                                                                         <  1:601396/140‑1 (MQ=255)                                              cTGTCGATGTGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAAc                                                                                        >  2:1338952/1‑140 (MQ=255)                                               tGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACg                                                                                       <  1:1070103/140‑1 (MQ=255)                                                  cGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCa                                                                                    <  2:776109/140‑1 (MQ=255)                                                      gAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTg                                                                                                <  2:256591/124‑1 (MQ=255)                                                      gAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTg                                                                                                >  1:256591/1‑124 (MQ=255)                                                      gAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACg                                                                                       <  2:30951/133‑1 (MQ=255)                                                      gAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACg                                                                                       >  1:30951/1‑133 (MQ=255)                                                      gAGTCTGTCACTGGTGCCGGGGTTCCTGCCGCTGCTGTTGATGGGGGGGTCGGCGGTCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGCTTTCTCTGACAGTAACGCCAatca                                                                                >  2:80374/1‑138 (MQ=255)                                                        gTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTTGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAatgatg                                                                              <  2:1189138/140‑1 (MQ=255)                                                        gTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAatgatg                                                                              >  1:91568/1‑140 (MQ=255)                                                         tCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGAtgt                                                                             >  1:1885133/1‑140 (MQ=255)                                                          cTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGAtgtg                                                                            <  1:3135/140‑1 (MQ=255)                                                           tGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGAtgtgt                                                                           >  1:615735/1‑140 (MQ=255)                                                                 tggtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCt                                                                       >  1:1787429/1‑138 (MQ=255)                                                                 tggtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCt                                                                       <  2:1787429/138‑1 (MQ=255)                                                                 tggtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTgg                                                                     >  2:1278534/1‑140 (MQ=255)                                                                  ggtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGa                                                                    <  1:1715469/140‑1 (MQ=255)                                                                   gtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGAt                                                                   >  1:640422/1‑140 (MQ=255)                                                                   gtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGAt                                                                   >  2:1627503/1‑140 (MQ=255)                                                                   gtggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGAt                                                                   >  1:996363/1‑140 (MQ=255)                                                                   gtggCGGTGGTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGAt                                                                   >  2:981240/1‑140 (MQ=255)                                                                     ggCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGc                                                                 <  2:1369154/140‑1 (MQ=255)                                                                       cGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGTTGCTg                                                               >  1:947987/1‑140 (MQ=255)                                                                               cTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAg                                                       <  2:1800437/140‑1 (MQ=255)                                                                                       gctgTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCcgc                                               <  1:1545715/140‑1 (MQ=255)                                                                                        ctgTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCcgcg                                              <  2:342562/140‑1 (MQ=255)                                                                                           ttGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGa                                           >  2:1108319/1‑140 (MQ=255)                                                                                                   cGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGc                                   >  1:831289/1‑140 (MQ=255)                                                                                                     gATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGa                                 >  2:458533/1‑140 (MQ=255)                                                                                                               ccGACTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGtt                       >  1:138720/1‑140 (MQ=255)                                                                                                                  aCTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGa                                 >  1:1659996/1‑127 (MQ=255)                                                                                                                  aCTGTTACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGa                                 <  2:1659996/127‑1 (MQ=255)                                                                                                                    tGTTACGCGAATTTGCCGTGGCTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGt                  <  1:931661/140‑1 (MQ=255)                                                                                                                       tACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGc               <  2:138720/140‑1 (MQ=255)                                                                                                                       tACGCGAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGc               >  2:1207053/1‑140 (MQ=255)                                                                                                                         cgcgAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCAt             <  1:569479/140‑1 (MQ=255)                                                                                                                         cgcgAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCAGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCAt             <  2:314161/140‑1 (MQ=255)                                                                                                                           cgAATTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGt           >  2:1366851/1‑140 (MQ=255)                                                                                                                              aTTTGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTgg        <  1:903275/140‑1 (MQ=255)                                                                                                                               tttGCCGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGt       <  1:47188/140‑1 (MQ=255)                                                                                                                                    cGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGTTGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGccc  <  2:947987/140‑1 (MQ=255)                                                                                                                                    cGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGccc  <  1:458533/140‑1 (MQ=255)                                                                                                                                    cGTGACTCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGccc  <  1:1010220/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTGGTGGCGGTGTTCCTGCCGCTGCTGTTGATGGGCGGATTGCCGGGCCGACTGTTACGCGAATTTGCCGTGACGCTTTCTGTCGCCATTGGTATATCGTTGCTGGTTTCTCTGACATTAACGCCAATGATGTGTGGCTGGATGCTGAAAGCCAGCAAGCCGCGCGAGCAAAAGCGACTGCGTGGTTTTGGTCGCATGTTGGTAGCCC  >  NC_000913/2159646‑2159916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑