Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,319,376	G→T	R167R (CGC→CGA)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,319,376	0	G	T	100.0%	218.0 / NA	61	R167R (CGC→CGA)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/28);  total (33/28)

GGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAT  >  NC_000913/2319242‑2319505                                                                                                                                       |                                                                                                                                  ggCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTc                                                                                                                              <  2:76073/140‑1 (MQ=255)   cGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCa                                                                                                                            <  2:998547/140‑1 (MQ=255)   cGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCa                                                                                                                            >  2:746015/1‑140 (MQ=255)    gTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCAc                                                                                                                           <  1:940278/140‑1 (MQ=255)    gTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCAc                                                                                                                           >  1:352529/1‑140 (MQ=255)     tCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACt                                                                                                                          >  1:1021160/1‑140 (MQ=255)        aCGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGc                                                                                                                       <  1:1163260/140‑1 (MQ=255)         cgcgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTc                                                                                                                              >  2:1024088/1‑132 (MQ=255)         cgcgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTc                                                                                                                              <  1:1024088/132‑1 (MQ=255)         cgcgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCa                                                                                                                      >  1:1016450/1‑140 (MQ=255)            gTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCatat                                                                                                                   >  1:976650/1‑140 (MQ=255)            gTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTCATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCatat                                                                                                                   >  2:212670/1‑140 (MQ=255)                  aTACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTTATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCa                                                                                                             >  2:724078/1‑140 (MQ=255)                      ccGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGAcc                                                                                                         >  1:97538/1‑140 (MQ=255)                          cGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGaaa                                                                                                     >  2:731087/1‑140 (MQ=255)                          cGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGaaa                                                                                                     >  2:822014/1‑140 (MQ=255)                              aGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTgg                                                                                                 <  2:976650/140‑1 (MQ=255)                              aGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTgg                                                                                                 <  1:723528/140‑1 (MQ=255)                                ggCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGcc                                                                                               <  1:822014/140‑1 (MQ=255)                                ggCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTCATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGcc                                                                                               <  1:212670/140‑1 (MQ=255)                                   gcgGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg                                                                                            <  1:746015/140‑1 (MQ=255)                                    cgGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGc                                                                                           <  2:897472/140‑1 (MQ=255)                                    cgGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGc                                                                                           <  2:515655/140‑1 (MQ=255)                                     ggACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTTATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCa                                                                                          <  1:724078/140‑1 (MQ=255)                                     ggACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCa                                                                                          <  2:327945/140‑1 (MQ=255)                                     ggACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCa                                                                                          <  2:709038/140‑1 (MQ=255)                                           tCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGtt                                                                                    >  2:97183/1‑140 (MQ=255)                                            cGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTa                                                                                   >  2:997537/1‑140 (MQ=255)                                               tGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCg                                                                                <  1:97183/140‑1 (MQ=255)                                                   ccAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGc                                                                            >  1:455267/1‑140 (MQ=255)                                                            gTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTaaaa                                                                   >  2:1083484/1‑140 (MQ=255)                                                            gTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTaaaa                                                                   >  2:190757/1‑140 (MQ=255)                                                                cTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATAc                                                               <  2:97538/140‑1 (MQ=255)                                                                cTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATAc                                                               <  1:34145/140‑1 (MQ=255)                                                                cTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATAc                                                               <  2:1021160/140‑1 (MQ=255)                                                                cTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATAc                                                               <  2:352529/140‑1 (MQ=255)                                                                 tGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATAcc                                                              <  2:121291/140‑1 (MQ=255)                                                                       tATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGttt                                                        <  1:197817/140‑1 (MQ=255)                                                                             cTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCg                                                  >  2:546274/1‑140 (MQ=255)                                                                                   gtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGt                                                          >  2:993333/1‑126 (MQ=255)                                                                                   gtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGt                                                          <  1:993333/126‑1 (MQ=255)                                                                                    tgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCg                                           <  2:455267/140‑1 (MQ=255)                                                                                          tCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGaaa                                     >  1:827489/1‑140 (MQ=255)                                                                                           cGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGaaaa                                    >  1:603546/1‑140 (MQ=255)                                                                                                tttattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTAt                               >  1:76578/1‑140 (MQ=255)                                                                                                   attGATGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATcgc                            >  1:1038005/1‑140 (MQ=255)                                                                                                        tGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGTATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAg                       >  2:697582/1‑140 (MQ=255)                                                                                                        tGCGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAg                       >  2:500643/1‑140 (MQ=255)                                                                                                          cGTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATTCCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTa                     >  2:776769/1‑140 (MQ=255)                                                                                                           gTTCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAg                    >  1:120284/1‑140 (MQ=255)                                                                                                            ttCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTa                     <  2:268259/138‑1 (MQ=255)                                                                                                            ttCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTa                     >  1:268259/1‑138 (MQ=255)                                                                                                            ttCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAgc                   >  2:945768/1‑140 (MQ=255)                                                                                                            ttCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAgc                   >  1:317509/1‑140 (MQ=255)                                                                                                            ttCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAgc                   >  1:165414/1‑140 (MQ=255)                                                                                                            ttCATGCAAAGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAga                   >  1:526933/1‑139 (MQ=255)                                                                                                                    aaGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAAc           >  2:1108477/1‑140 (MQ=255)                                                                                                                    aaGCTTTCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAAc           <  2:989800/140‑1 (MQ=255)                                                                                                                          tCAACGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGcc     >  2:503504/1‑140 (MQ=255)                                                                                                                             aCGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt  <  1:997537/140‑1 (MQ=255)                                                                                                                             aCGCGGTATCTCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt  <  1:546274/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  GGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAT  >  NC_000913/2319242‑2319505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑