Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,974,662	C→A	L66M (CTG→ATG)	rffC →	dTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,974,662	0	C	A	100.0%	208.1 / NA	58	L66M (CTG→ATG)	rffC	dTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/29);  total (29/29)

ACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCCTGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGTTTTCGT  >  NC_000913/3974525‑3974796                                                                                                                                          |                                                                                                                                       aCAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATg                                                                                                                                      >  2:297519/1‑140 (MQ=255)    gcgcCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTa                                                                                                                                   <  2:777647/140‑1 (MQ=255)     cgcCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAg                                                                                                                                  >  1:657881/1‑140 (MQ=255)     cgcCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAg                                                                                                                                  >  2:17996/1‑140 (MQ=255)     cgcCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAg                                                                                                                                  >  2:410074/1‑140 (MQ=255)     cgcCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAg                                                                                                                                  >  2:528484/1‑140 (MQ=255)      gcCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGa                                                                                                                                 >  2:1153702/1‑140 (MQ=255)          tCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGt                                                                                                                             >  1:109542/1‑140 (MQ=255)          tCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGt                                                                                                                             <  2:30176/140‑1 (MQ=255)              gcgcATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAg                                                                                                                         <  2:657881/140‑1 (MQ=255)                    tACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGAtt                                                                                                                   >  2:14082/1‑140 (MQ=255)                       gTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTgg                                                                                                                <  1:297519/140‑1 (MQ=255)                          tGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAGCTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             <  2:480483/140‑1 (MQ=255)                          tGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             <  1:356365/140‑1 (MQ=255)                          tGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             <  1:14082/140‑1 (MQ=255)                            aaGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTa                                                                                                           >  2:680828/1‑140 (MQ=255)                                aCCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCg                                                                                                       >  2:772787/1‑140 (MQ=255)                                aCCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCg                                                                                                       <  1:680828/140‑1 (MQ=255)                                 ccGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             <  1:498829/133‑1 (MQ=255)                                 ccGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             >  2:498829/1‑133 (MQ=255)                                 ccGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             >  2:934503/1‑133 (MQ=255)                                 ccGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGgcgc                                                                                                             <  1:934503/133‑1 (MQ=255)                                  cGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTg                                                                                                     <  1:1153596/140‑1 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   >  2:736583/1‑140 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   >  2:551061/1‑140 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   <  2:109542/140‑1 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   <  1:409252/140‑1 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   <  1:551061/140‑1 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   <  1:318230/140‑1 (MQ=255)                                    cTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGAGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGGGAAGTTGATTTGGCGCTACCCGTGaa                                                                                                   >  1:429714/1‑140 (MQ=255)                                      ccTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACa                                                                                                 <  1:410074/140‑1 (MQ=255)                                        tGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAAt                                                                                               <  2:380952/140‑1 (MQ=255)                                         gACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATg                                                                                              >  1:491794/1‑140 (MQ=255)                                         gACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATg                                                                                              <  1:736583/140‑1 (MQ=255)                                          aCGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGc                                                                                             <  1:1153702/140‑1 (MQ=255)                                            gCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCa                                                                                           >  1:684718/1‑140 (MQ=255)                                              cAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGt                                                                                         >  2:1117067/1‑140 (MQ=255)                                                      ttAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCgg                                                                                 <  2:864839/140‑1 (MQ=255)                                                         gcgcCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGc                                                                              <  2:270507/140‑1 (MQ=255)                                                          cgcCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCt                                                                             <  2:670726/140‑1 (MQ=255)                                                                    aCGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGcac                                                                   <  1:528484/140‑1 (MQ=255)                                                                     cGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGcaca                                                                  <  1:17996/140‑1 (MQ=255)                                                                            aGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCT‑CCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCg                                                          >  1:48394/1‑140 (MQ=255)                                                                                       gCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGc                                                <  1:772787/140‑1 (MQ=255)                                                                                       gCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGc                                                <  2:972138/140‑1 (MQ=255)                                                                                           cATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCT‑CCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTAc                                           <  2:48394/140‑1 (MQ=255)                                                                                                       gTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGcc                                >  2:843281/1‑140 (MQ=255)                                                                                                          aaCTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAgc                             >  2:363017/1‑140 (MQ=255)                                                                                                          aaCTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAgc                             >  1:640507/1‑140 (MQ=255)                                                                                                          aaCTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAgc                             >  2:934922/1‑140 (MQ=255)                                                                                                           aCTGGATGCCCTGCAACAGCTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAACCAgcg                            >  2:724197/1‑140 (MQ=255)                                                                                                                              cTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCg         >  1:556127/1‑140 (MQ=255)                                                                                                                              cTGGGATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCg         >  1:958858/1‑140 (MQ=255)                                                                                                                                gggATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGtt       >  1:219183/1‑140 (MQ=255)                                                                                                                                  gATTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGtttt     >  1:1039413/1‑140 (MQ=255)                                                                                                                                   aTTCTCCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGTTTTc    <  2:44577/140‑1 (MQ=255)                                                                                                                                     tctcCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGTTTTCGt  <  1:934922/140‑1 (MQ=255)                                                                                                                                      ctcCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGTTTTCGt  >  2:519630/1‑139 (MQ=255)                                                                                                                                      ctcCATGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGTTTTCGt  <  1:519630/139‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       ACAGCGCCATCGTGCGCATTACGTCTGAAGCACCGCTCCTGACGCCAGACGCGTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGGGTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCCTGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCCAGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAGCCGTTTTCGT  >  NC_000913/3974525‑3974796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑