Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,143	T→G	L328R (CTT→CGT)	pepQ →	Xaa‑Pro dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,143	0	T	G	100.0%	361.5 / NA	99	L328R (CTT→CGT)	pepQ	Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (49/50);  total (49/50)

GCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTACCTGCGCTGCACCCG  >  NC_000913/4032007‑4032278                                                                                                                                         |                                                                                                                                        gcgcTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTAc                                                                                                                                      >  1:637792/1‑140 (MQ=255)  cgcTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTAcc                                                                                                                                     >  1:411412/1‑140 (MQ=255)    cTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCgg                                                                                                                                   <  1:999803/140‑1 (MQ=255)     tGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCggg                                                                                                                                  <  1:633185/140‑1 (MQ=255)       aTCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGcc                                                                                                                                >  1:41508/1‑140 (MQ=255)        tCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCg                                                                                                                               >  2:279466/1‑140 (MQ=255)            caccaTGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTa                                                                                                                           >  2:981996/1‑140 (MQ=255)            caccaTGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTa                                                                                                                           >  1:193740/1‑140 (MQ=255)              ccaTGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATg                                                                                                                         <  1:390645/140‑1 (MQ=255)               caTGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGc                                                                                                                        >  2:784460/1‑140 (MQ=255)                 tGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCg                                                                                                                      >  1:404940/1‑140 (MQ=255)                  gAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGc                                                                                                                     <  1:279466/140‑1 (MQ=255)                  gAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGc                                                                                                                     <  2:895566/140‑1 (MQ=255)                   aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    <  1:102478/140‑1 (MQ=255)                   aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    >  2:712928/1‑140 (MQ=255)                   aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    <  2:707577/140‑1 (MQ=255)                   aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    <  2:1095298/140‑1 (MQ=255)                   aaaGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    <  2:39041/140‑1 (MQ=255)                        aGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCAt                                                                                                                   <  1:200809/136‑1 (MQ=255)                        aGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCAt                                                                                                                   >  2:200809/1‑136 (MQ=255)                        aGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTa                                                                                                               <  1:459834/140‑1 (MQ=255)                         ggCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    >  2:138769/1‑134 (MQ=255)                         ggCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCa                                                                                                                    <  1:138769/134‑1 (MQ=255)                         ggCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTAt                                                                                                              >  1:252915/1‑140 (MQ=255)                         ggCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTAt                                                                                                              >  2:177194/1‑140 (MQ=255)                         ggCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTAt                                                                                                              <  2:19350/140‑1 (MQ=255)                          gCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATc                                                                                                             >  1:515548/1‑140 (MQ=255)                               aGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCa                                                                                                        <  2:278404/140‑1 (MQ=255)                               aGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCa                                                                                                        <  1:166932/140‑1 (MQ=255)                               aGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCa                                                                                                        >  2:942094/1‑140 (MQ=255)                                    tgtgGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGc                                                                                                   >  2:259056/1‑140 (MQ=255)                                     gtgGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCt                                                                                                  <  2:155027/140‑1 (MQ=255)                                       ggATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTgg                                                                                                <  1:418085/140‑1 (MQ=255)                                          ttACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGcc                                                                                             <  2:271903/140‑1 (MQ=255)                                          ttACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGcc                                                                                             >  1:369858/1‑140 (MQ=255)                                           tACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCt                                                                                            >  2:176097/1‑140 (MQ=255)                                             ccACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGc                                                                                          <  2:876891/140‑1 (MQ=255)                                             ccACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGc                                                                                          <  1:712928/140‑1 (MQ=255)                                             ccACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGc                                                                                          <  2:515548/140‑1 (MQ=255)                                             ccACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGc                                                                                          <  2:637792/140‑1 (MQ=255)                                               acaTCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAg                                                                                        <  2:729532/140‑1 (MQ=255)                                               acaTCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAg                                                                                        <  2:252915/140‑1 (MQ=255)                                                caTCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAgg                                                                                       >  2:689513/1‑140 (MQ=255)                                                  tCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTg                                                                                     <  2:193740/140‑1 (MQ=255)                                                   ccAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGcc                                                                                             >  2:102797/1‑131 (MQ=255)                                                   ccAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGcc                                                                                             <  1:102797/131‑1 (MQ=255)                                                       ttCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGa                                                                                >  2:974198/1‑140 (MQ=255)                                                       ttCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGa                                                                                >  1:70987/1‑140 (MQ=255)                                                       ttCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGa                                                                                >  1:1027346/1‑140 (MQ=255)                                                       ttCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGa                                                                                >  1:1009077/1‑140 (MQ=255)                                                       ttCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGa                                                                                >  1:570098/1‑140 (MQ=255)                                                        tCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGAc                                                                               <  1:463886/140‑1 (MQ=255)                                                        tCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGAc                                                                               <  2:369858/140‑1 (MQ=255)                                                           aTCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTc                                                                            >  1:788438/1‑140 (MQ=255)                                                            tCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCg                                                                           >  2:611315/1‑140 (MQ=255)                                                            tCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCg                                                                           >  2:188270/1‑140 (MQ=255)                                                             cAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                                          <  1:177194/140‑1 (MQ=255)                                                             cAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                                          <  2:1018748/140‑1 (MQ=255)                                                             cAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                                          <  2:404940/140‑1 (MQ=255)                                                               gcgcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTg                                                                        >  2:1111404/1‑140 (MQ=255)                                                                cgcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTgg                                                                       >  1:349452/1‑140 (MQ=255)                                                                 gcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGTTATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGt                                                                      >  1:404644/1‑140 (MQ=255)                                                                 gcATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGt                                                                      >  1:564513/1‑140 (MQ=255)                                                                    tCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                                          >  2:208945/1‑133 (MQ=255)                                                                    tCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGc                                                                          <  1:208945/133‑1 (MQ=255)                                                                       ccAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATg                                                                <  1:1138399/140‑1 (MQ=255)                                                                       ccAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATg                                                                <  1:981996/140‑1 (MQ=255)                                                                       ccAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATg                                                                <  2:972714/140‑1 (MQ=255)                                                                        cAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGc                                                               <  1:495151/140‑1 (MQ=255)                                                                        cAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGc                                                               <  2:449810/140‑1 (MQ=255)                                                                          aaCTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAg                                                             <  2:41508/140‑1 (MQ=255)                                                                               ctgcGTAAACATCAAATCATCCCCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGgatga                                                        <  2:688352/140‑1 (MQ=255)                                                                               ctgcGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGgatga                                                        >  2:705693/1‑140 (MQ=255)                                                                                 gcGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATa                                                      >  2:399267/1‑140 (MQ=255)                                                                                 gcGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATa                                                      <  1:974198/140‑1 (MQ=255)                                                                                  cGTAAACATCAAATCATCACCGTTATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAg                                                     <  2:404644/140‑1 (MQ=255)                                                                                  cGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAg                                                     >  2:190130/1‑140 (MQ=255)                                                                                  cGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAg                                                     >  1:843033/1‑140 (MQ=255)                                                                                    tAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCg                                                   <  2:184480/140‑1 (MQ=255)                                                                                    tAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCg                                                   >  1:316922/1‑140 (MQ=255)                                                                                            aaaTCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCAc                                           >  2:480879/1‑140 (MQ=255)                                                                                               tcatcaCCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTc                                        <  2:411412/140‑1 (MQ=255)                                                                                                 atcaCCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTcgc                                      >  1:277670/1‑140 (MQ=255)                                                                                                        atatGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCg                               >  1:921922/1‑140 (MQ=255)                                                                                                        atatGAGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCg                               >  2:659391/1‑140 (MQ=255)                                                                                                             aGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCaaa                          >  2:939193/1‑140 (MQ=255)                                                                                                             aGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCaaa                          >  1:834462/1‑140 (MQ=255)                                                                                                             aGTGAAGAAGCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGACCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCaaa                          <  1:1058695/140‑1 (MQ=255)                                                                                                                  agaagCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCAcc                                <  2:999021/129‑1 (MQ=255)                                                                                                                  agaagCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCAcc                                >  1:999021/1‑129 (MQ=255)                                                                                                                  agaagCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCg                               >  1:381686/1‑130 (MQ=255)                                                                                                                  agaagCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCg                               <  2:381686/130‑1 (MQ=255)                                                                                                                   gaagCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATcc                    <  1:659391/140‑1 (MQ=255)                                                                                                                   gaagCGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATcc                    >  2:161377/1‑140 (MQ=255)                                                                                                                       cGATGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTAc                >  2:749527/1‑140 (MQ=255)                                                                                                                         aTGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTACCt              >  1:875740/1‑140 (MQ=255)                                                                                                                          tGGTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTACCTg             <  1:399267/140‑1 (MQ=255)                                                                                                                            gTCGAAAACGATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCAGCAAAATATCCGTACCTgcg           <  1:784460/140‑1 (MQ=255)                                                                                                                                     gATCGTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTACCTGCGCTGCACCCg  <  1:731669/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAAAACGATCTTACCGGGCCGTTTATGCCGCATGGTATCGGCCATCCGCTGGGCCTGCAGGTGCATGACGTCGCTGGTTTTATGCAGGATGATAGCGGTACGCACCTCGCGGCACCGGCAAAATATCCGTACCTGCGCTGCACCCG  >  NC_000913/4032007‑4032278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑