Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,938,954	G→T	A436S (GCA→TCA)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,938,954	0	G	T	100.0%	239.7 / NA	68	A436S (GCA→TCA)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/45);  total (23/45)

TACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTAGCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTA  >  NC_000913/1938818‑1939085                                                                                                                                         |                                                                                                                                    tACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTatcag                                                                                                                                  >  1:1035176/1‑140 (MQ=255)   ccGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCt                                                                                                                                <  2:767625/140‑1 (MQ=255)       gcTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCa                                                                                                                            <  2:482065/140‑1 (MQ=255)       gcTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCa                                                                                                                            >  1:40063/1‑140 (MQ=255)        cTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAg                                                                                                                           >  1:506078/1‑140 (MQ=255)          gatgaCCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCATTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCg                                                                                                                         <  1:258863/140‑1 (MQ=255)           atgaCCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGa                                                                                                                        <  1:865690/140‑1 (MQ=255)            tgaCCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGaa                                                                                                                       <  2:1035176/140‑1 (MQ=255)                cTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCgg                                                                                                                   <  2:830080/140‑1 (MQ=255)                cTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCgg                                                                                                                   <  2:506078/140‑1 (MQ=255)                   ccGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTa                                                                                                                >  2:26077/1‑140 (MQ=255)                     gTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAAt                                                                                                              >  2:966059/1‑140 (MQ=255)                           gCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATctgctg                                                                                                        >  2:526560/1‑140 (MQ=255)                           gCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATctgctg                                                                                                        <  2:1063965/140‑1 (MQ=255)                             tctGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTgcg                                                                                                      >  2:761904/1‑140 (MQ=255)                                ggTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGa                                                                                                   >  1:1098382/1‑140 (MQ=255)                                 gTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGAt                                                                                                  >  2:75861/1‑140 (MQ=255)                                 gTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGAt                                                                                                  >  2:431980/1‑140 (MQ=255)                                   cTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATaa                                                                                                <  1:916421/140‑1 (MQ=255)                                     gCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAg                                                                                              <  1:183712/140‑1 (MQ=255)                                      ccAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAgg                                                                                             >  2:981792/1‑140 (MQ=255)                                       cAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGt                                                                                            <  1:26077/140‑1 (MQ=255)                                             tCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTg                                                                                      <  1:422943/140‑1 (MQ=255)                                              cGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGa                                                                                     <  1:998708/140‑1 (MQ=255)                                               gCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGAt                                                                                    <  2:40063/140‑1 (MQ=255)                                               gCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGAt                                                                                    <  2:1144593/140‑1 (MQ=255)                                                     tCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTgg                                                                              <  1:800993/140‑1 (MQ=255)                                                             tGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTgg                                                                      <  1:981792/140‑1 (MQ=255)                                                                aCGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGa                                                                   <  1:75861/140‑1 (MQ=255)                                                                       cTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCAc                                                            >  1:1129134/1‑140 (MQ=255)                                                                            ccTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAgg                                                       <  1:431980/140‑1 (MQ=255)                                                                               gACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCg                                                    <  2:680057/140‑1 (MQ=255)                                                                                 cTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGAc                                                  <  2:982861/140‑1 (MQ=255)                                                                                  tGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACg                                                 >  2:172486/1‑140 (MQ=255)                                                                                   gCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGt                                                <  2:1052022/140‑1 (MQ=255)                                                                                       tctATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGtgatg                                            <  1:664867/140‑1 (MQ=255)                                                                                            cGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTAc                                       >  2:582706/1‑140 (MQ=255)                                                                                             gTGACGTTACGCCGGTGCACTTTGATAGCGCCTATGGCGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTAcc                                      <  1:212201/140‑1 (MQ=255)                                                                                              tGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCg                                     <  1:74585/140‑1 (MQ=255)                                                                                              tGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCg                                     >  2:74585/1‑140 (MQ=255)                                                                                              tGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCg                                     <  2:1052213/140‑1 (MQ=255)                                                                                                gCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTg                                   >  1:1034676/1‑140 (MQ=255)                                                                                                gCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTg                                   >  1:293879/1‑140 (MQ=255)                                                                                                gCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTg                                   >  2:471374/1‑140 (MQ=255)                                                                                                 cGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTgg                                  >  1:233035/1‑140 (MQ=255)                                                                                                 cGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTgg                                  >  2:101923/1‑140 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  2:1034676/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:966059/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:126212/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:526560/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:57096/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:571116/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                >  1:1072283/1‑140 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:582706/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  2:1129134/140‑1 (MQ=255)                                                                                                   ttACGCCGGTGCACGTTGAGAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGt                                <  1:81669/140‑1 (MQ=255)                                                                                                    tACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGtt                               <  1:369016/140‑1 (MQ=255)                                                                                                    tACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGtt                               <  1:172486/140‑1 (MQ=255)                                                                                                     aCGCCGGTGCACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTc                              <  1:58495/140‑1 (MQ=255)                                                                                                              cACTTTGATAGCGCTAATGACGGCGTATCAGCTGCCAGCGCAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCGCCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAAtcc                     <  2:563449/140‑3 (MQ=255)                                                                                                                        gcgcTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTAtt           >  2:934451/1‑140 (MQ=255)                                                                                                                         cgcTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTAttt          >  2:84664/1‑140 (MQ=255)                                                                                                                          gcTAATGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTAtttt         <  2:1098382/140‑1 (MQ=255)                                                                                                                             aaTGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAAc      <  1:260529/140‑1 (MQ=255)                                                                                                                             aaTGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAAc      >  1:522256/1‑140 (MQ=255)                                                                                                                             aaTGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAAc      <  1:58794/140‑1 (MQ=255)                                                                                                                               tGACGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACgg    <  1:65711/140‑1 (MQ=255)                                                                                                                                 aCGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTa  <  1:934451/140‑1 (MQ=255)                                                                                                                                 aCGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTa  <  2:563122/140‑1 (MQ=255)                                                                                                                                 aCGGCGTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTa  <  1:471374/140‑1 (MQ=255)                                                                                                                                      gTATCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTAttt          <  2:1134687/127‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGCCGGTGCACTTTGATAGCGCTAATGACGGCGTAGCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTGGTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTA  >  NC_000913/1938818‑1939085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑