Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	24.2%	121.7 / 28.7	66	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (29/21);  new base C (0/16);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.85e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445419‑445692                                                                                                                                            |                                                                                                                                        tAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTTCGCCCGCGAGAAATa                                                                                                                                         >  2:1979442/1‑140 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:2149053/140‑1 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:2247613/140‑1 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:917977/140‑1 (MQ=255)       aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:127170/140‑1 (MQ=255)         gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                 <  2:1010168/140‑1 (MQ=255)         gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                 <  2:413814/140‑1 (MQ=255)         gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                 <  2:217624/140‑1 (MQ=255)                   cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTCAAAC‑a                                                                                                                      >  1:94017/1‑140 (MQ=255)                   cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑a                                                                                                                      <  2:2205090/140‑1 (MQ=255)                   cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑a                                                                                                                      <  2:2097289/140‑1 (MQ=255)                   cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑a                                                                                                                      <  1:1979442/140‑1 (MQ=255)                    gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑At                                                                                                                     <  1:295392/140‑1 (MQ=255)                    gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑At                                                                                                                     <  2:1091500/140‑1 (MQ=255)                     acaggTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATg                                                                                                                    <  1:769594/136‑1 (MQ=255)                       tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGGGGCCGTCAGCCACACCCCGGCCAGCATTTTGCCCGCGCGAAAAAACCCCCGCCCCTCAAAC‑aatcc                                                                                                                  >  2:1186980/1‑136 (MQ=255)                       tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGGCAAAC‑ATGcc                                                                                                                  >  1:670109/1‑140 (MQ=255)                       tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCCGCCTTTCGCCCGCGAGAAAAACCCCCCGACCGCCCAAC‑AAGcc                                                                                                                  >  2:1993481/1‑140 (MQ=255)                           ttGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTa                                                                                                              <  2:189823/140‑1 (MQ=255)                                gCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCa                                                                                                         <  1:2440284/140‑1 (MQ=255)                                 cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAg                                                                                                        <  1:480103/140‑1 (MQ=255)                                    tGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATTGGACTCGCGGCTGTCAGCCACACCGCGGCCCGCAATTCGCCAGCGGGAAAAAACCCCCTACCGCCAAAA‑AAGCCGGTAATCCacctt                                                                                                     >  2:769594/1‑136 (MQ=255)                                      ttCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAgccgcc                                                                                                   <  1:1994400/140‑1 (MQ=255)                                        cGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAAAAACCCCCGACCGTCAAAC‑ATGCCGTTAATCCAGCCGcccc                                                                                                 >  2:1919324/1‑139 (MQ=255)                                         gCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAg                                                                                                >  1:228421/1‑140 (MQ=255)                                         gCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAg                                                                                                >  2:1108550/1‑140 (MQ=255)                                                   aaaTGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGCCTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCa                                                                                      <  2:571555/139‑1 (MQ=255)                                                        cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                 >  1:419397/1‑140 (MQ=255)                                                        cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACGGCGGCCAGCATTGCCCCAGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                 <  2:1603823/140‑1 (MQ=255)                                                        cgaaacctgctgACATACGGGGTTTTTTTCAGGGTACTGGCGCCTGTCAGCCACACTGCGGCCAGCTTTGCGCCAGCGAGAAATCCCCCCTGACCGTCAACC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                 <  2:419397/134‑1 (MQ=255)                                                          cAAACTGCTGACATACGGCGTTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCTTTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                               <  2:94017/140‑1 (MQ=255)                                                                    aCATCCGGCGTTTTTTTCAGGGTCCGGGCGACTGTCAGCCACCCTGCGGCCAGCTTTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                     <  2:1686105/140‑1 (MQ=255)                                                                    aCATCCGGCGGTTCTTTCATGGTACGGGCGCCTGCCAGCCACACTGCGCCAAGCATTGCGCCAGCGAAAAATCCCCCCGGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                     <  2:1686106/140‑1 (MQ=255)                                                                         ggggggTTTTTTCAGGTTTCGGGCGATTTTCACCCACCCGGCGGCAAGCATTGCGCCAGAGAAAAAACCCCCCGGCCCGAAAAAC‑AGGCGGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                <  2:1189249/136‑1 (MQ=255)                                                                         gggcggTTTTTTCATGGTCCTGGGGCCTGTCAGCCACCCTGCGGCAAGCATGGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                <  2:1190234/139‑1 (MQ=255)                                                                         cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                <  1:1919324/140‑1 (MQ=255)                                                                         cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                >  2:1607892/1‑140 (MQ=255)                                                                              gTTCTTTTATGGTACTGGCGGCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCTACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCCGCGAACCGCCCATCGCCACGCCAAGAAAatggctgggg                                                           >  2:362171/1‑138 (MQ=255)                                                                              gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                           >  1:638172/1‑140 (MQ=255)                                                                               ttCTTTCATGGTACTGGCGCCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgg                                                          <  1:1925360/140‑1 (MQ=255)                                                                                ttttttATGGTACTGGCGACTGTCAGCCCCACTGCGGCCCGCATTTCGCCCGCGAGAAAAACCCCCCGACCGTCAAAC‑AAGCCGGTTAACCAGCCGCCCAGCGAACCCCCCAACGCCCCCCCCAGAAAAAGGCtgggggg                                                         >  2:1209807/3‑139 (MQ=255)                                                                                tCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGGCAGCAATTCGGCAGCGAGAAATACCCCCCGACCGTCAAAC‑ATGCCGTTTATCCAGCCGCCCCACGCACCGCCCATCGCCACGCCAAGAAACTGGCtgggggg                                                         >  2:1925360/1‑139 (MQ=255)                                                                                  tttCATGGTACGGGCGCCTGTCAGCCACACTGCGGCAAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTAAACC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGt                                                       <  2:1029161/140‑1 (MQ=255)                                                                                      aTGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaaaa                                                   >  1:1312934/1‑139 (MQ=255)                                                                                        ggTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAacac                                                 >  2:1355444/1‑140 (MQ=255)                                                                                         gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                >  1:2067327/1‑140 (MQ=255)                                                                                         gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                >  1:2149794/1‑140 (MQ=255)                                                                                         gTACGGGCGCCTTTCAGCCACACGGCGGCCAGCATGGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                <  1:1475954/140‑1 (MQ=255)                                                                                          acggggCGACTGCCACCCACACTGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                               <  2:2250682/136‑1 (MQ=255)                                                                                            cTGGCGATTGCCAGCCACCCTGCGGCCAGTTTGGCGCCAGCAAAAAATACCCCCTGCCCGACAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                             <  2:873849/140‑1 (MQ=255)                                                                                            cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAGAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                             <  1:1368158/140‑1 (MQ=255)                                                                                             tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                            >  2:1414843/1‑140 (MQ=255)                                                                                             tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                            >  2:155354/1‑140 (MQ=255)                                                                                              ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                           >  1:997375/1‑140 (MQ=255)                                                                                               gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                          >  2:1621490/1‑140 (MQ=255)                                                                                                   ccgTCAGCCAAACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      >  1:827378/3‑140 (MQ=255)                                                                                                   cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTAACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  1:123408/140‑1 (MQ=255)                                                                                                   cTGTCACCCACACGGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  1:1209807/140‑1 (MQ=255)                                                                                                   cTGCCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCTTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  2:1529903/140‑1 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  1:949482/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  1:1529903/1‑140 (MQ=255)                                                                                                    tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:2222348/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACTCCCATCGCCGTACCt                                   >  2:2069289/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  2:836035/1‑140 (MQ=255)                                                                                                      tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  2:1724426/1‑140 (MQ=255)                                                                                                         gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAccctgt                                >  1:2250682/1‑136 (MQ=255)                                                                                                         gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                >  2:405782/1‑140 (MQ=255)                                                                                                           cccccggCGGCCAGCATGGCCCCAGCGAAAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                              <  2:997375/134‑1 (MQ=255)                                                                                                           cacaCTGCGCCCAGCTTTCCGCCAGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                              <  2:904224/140‑1 (MQ=255)                                                                                                                tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                         >  1:904224/1‑140 (MQ=255)                                                                                                                  cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       >  2:580013/1‑140 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     <  1:1993481/140‑1 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     <  1:405782/140‑1 (MQ=255)                                                                                                                    gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     >  1:1257323/1‑140 (MQ=255)                                                                                                                     ccAGAATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                    <  1:2012729/140‑1 (MQ=255)                                                                                                                       aGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  2:1257323/140‑1 (MQ=255)                                                                                                                       aGCATTGCCCCAGCGAGAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  2:1526633/140‑1 (MQ=255)                                                                                                                       aGCATGGCGCAAGCGAAAAATCCCCCCTGCCCGTAAACC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  2:670109/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  1:53006/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                >  2:1738973/1‑140 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  1:1414843/140‑1 (MQ=255)                                                                                                                         cATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                >  1:1980536/1‑140 (MQ=255)                                                                                                                           ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:1621490/140‑1 (MQ=255)                                                                                                                           tggcgcGAGCGAGAAATCCCCCTTGACCG‑CAAACAATGCCGTTAACCCAGCCGCCAAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  2:1641085/138‑1 (MQ=255)                                                                                                                           tggcgcCAGCGAGAAATCCCCCCTGACCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  2:2067327/138‑1 (MQ=255)                                                                                                                             gcgcCAGCGAGAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:362171/140‑1 (MQ=255)                                                                                                                             gcgcCAGCGAGAAATCCCCCCTGACCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:1012808/140‑1 (MQ=255)                                                                                                                             gcgcCAGCGAGAAATCCCCCCTACCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:2045338/140‑1 (MQ=255)                                                                                                                             gcgcCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:1355444/140‑1 (MQ=255)                                                                                                                             gcgcCAGCGAAAAATCCCCCCTGACCTACAACC‑ATGCCTTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  2:894878/140‑1 (MQ=255)                                                                                                                                ccAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACt         <  1:836035/140‑1 (MQ=255)                                                                                                                                  aGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       >  1:894878/1‑140 (MQ=255)                                                                                                                                  aGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  1:2222348/140‑1 (MQ=255)                                                                                                                                   gCGAGAAATCCCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:455961/132‑1 (MQ=255)                                                                                                                                   gCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              >  2:455961/1‑132 (MQ=255)                                                                                                                                   gCGAAAAATCCCCCCTGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt      <  2:949482/140‑1 (MQ=255)                                                                                                                                       aaaaTCCCCCCGGCCCGTAAAAC‑ATGCCTTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt  <  2:2149794/139‑1 (MQ=255)                                                                                                                                       aaaaTCCCCCCGGCCCGTAAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt  <  2:638172/139‑1 (MQ=255)                                                                                                                                            |                                                                                                                                        TAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAAC‑ATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445419‑445692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑