Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,207	0	T	C	28.3%	106.8 / 21.4	60	S215G (AGT→GGT)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (40/3);  new base C (0/17);  total (40/20)
Fisher's exact test for biased strand distribution p-value = 2.94e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556068‑3556345                                                                                                                                            |                                                                                                                                           accTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACt                                                                                                                                            >  1:816779/1‑140 (MQ=255)   cTCGGCACTGACGCGCTTTTCACCGACGGCAAAAAAGCGTTCGGCGATATTTTCACTTTCGACTTCAAGCGAGACGGCATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcc                                                                                                                                          >  1:1425094/1‑140 (MQ=255)             aCGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                                >  1:2373203/1‑140 (MQ=255)               gcgtTTTCCACCGACGACAAAAAAGCGTTGGGTAATTTTTTCCTTTTCGACTTCAGGCGAGACGGTATTCCCCGGCCCCGGGTCGCGGGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAg                                                                                                                              <  2:2244984/140‑1 (MQ=255)                       ccccGACGACAAAAAAGCTTTGGGTAATTTTTTCCCTTCCGACTTAAAGCGAGAGGGTTTTCCCCGCCCCCTGGTCGCGGGGAAGTTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCa                                                                                                                      <  2:1678650/138‑1 (MQ=255)                       cACCGACGACAAAAAAGCGTTCGGTGATTTTTTCACTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCGGGTCGCGCGGCAGGTTATAAATATTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCa                                                                                                                      <  2:232641/140‑1 (MQ=255)                         cggccgaaAAAAAGGCTTTGGGTGTTTTTTTCACTTTCGATTTAAAGCGAGACGTTTTCCCCCGCCCCCTGGGCCGGCGGCGGTTTATGAATTTTCTGTTCAGGCGGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                    <  2:947982/136‑1 (MQ=255)                         ccgccgAAAAAAAGGCGTTTGGTGATATTTTCACTTTCGCCTCCAAGCGGGATTGTATCCCCGGGCCCGTGGTCGCGCGGTAGTTTATAAATATTCTGTTCATGAAGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                    <  2:1272502/140‑1 (MQ=255)                                          ttgggTGATTTTTTCCTTTTCGACTTCAAGGGAGACGTTATCCCCCGCCCCTGGGCCGCGCGGCGGTTTATGAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                   <  2:1906025/137‑1 (MQ=255)                                             ggTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGca                                                                                                >  2:169983/1‑140 (MQ=255)                                               tGATTTTTTCTTTTTCGCTTTCAAGCGAGAGGGTATTCCCCGCCCCCTGGTCGCGGGGCAGTTTATAAATTTTTTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:816779/140‑1 (MQ=255)                                               tGATTTTTTCATTTCCGATTCCAGGCGAGGCGGTATTCCGCGCCCCGGGGTCGCGCGGCAGGTTATGAATTTTCTGTTCAGGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:624759/140‑1 (MQ=255)                                               tGATTTTTTCACTTTCGCCTAAAAGGGAGACGGTATCCCCCGCCCCGGGGCCGCGGGGCGGGTTATAAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcaca                                                                                              <  2:1538653/140‑1 (MQ=255)                                                         aCTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                    >  1:351801/1‑140 (MQ=255)                                                           ttccgccTTCAAGGGAGACGTTATTCCCCGCCCCCGGGTCGCGCGGCAGTTTATAAATATTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                  <  2:2256033/134‑1 (MQ=255)                                                            ttCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAgct                                                                                 >  1:533257/1‑138 (MQ=255)                                                             tCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                >  1:2247246/1‑140 (MQ=255)                                                              cGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                               >  2:2228629/1‑140 (MQ=255)                                                                aCTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCTGCTAATAGAACTtc                                                                             >  1:2256033/1‑140 (MQ=255)                                                                    caggCGAGACGTTTTTCCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                         <  2:2128274/137‑1 (MQ=255)                                                                              tttATTACCCCCCCCCTGGACGCGCGGAAGATAAATAATTTCCTGTTCTAGCAGAGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                               <  2:2346870/138‑1 (MQ=255)                                                                              ggtttgccccGCCCCCGGGCCGCGGGGCAGGTTAAAATTTTTCTGTTAAGGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                               <  2:2295610/133‑1 (MQ=255)                                                                              ggTATTACCCGCCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGca                                                               <  1:2121462/140‑1 (MQ=255)                                                                                   tACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                          >  2:1307373/1‑140 (MQ=255)                                                                                        ggCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                     >  2:1076180/1‑140 (MQ=255)                                                                                          ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  1:283306/1‑140 (MQ=255)                                                                                          ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  1:2331518/1‑140 (MQ=255)                                                                                          ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                   >  2:670493/1‑140 (MQ=255)                                                                                               ggTCGGGCGGAAGGTTATGAATTTTCTGTGCAGGGGGGGAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                              <  2:178266/140‑1 (MQ=255)                                                                                               ggTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                              >  2:1732939/1‑140 (MQ=255)                                                                                                gTCGCGCGGCAGGCTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGc                                             >  1:425150/1‑140 (MQ=255)                                                                                                   gcgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCaa                                          >  1:692448/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  1:820555/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  2:253639/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  1:1314723/1‑140 (MQ=255)                                                                                                    cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                         >  2:576027/1‑140 (MQ=255)                                                                                                         aagtttATAAATTTTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    <  2:692448/136‑1 (MQ=255)                                                                                                           ggTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg                                  <  1:253639/140‑1 (MQ=255)                                                                                                                      ttttGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                       <  1:324326/137‑1 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCGCGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    >  2:2436721/1‑127 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCGCGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    <  1:2436721/127‑1 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    >  2:2366392/1‑127 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                       >  1:2439547/1‑140 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                       >  1:1309561/1‑140 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                    <  1:2366392/127‑1 (MQ=255)                                                                                                                      ttCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCATCTAATAGAACTTCTCCACGCACCTGCACAATATTCCCGCGCTCGCCAAGTCGCAAGGTGTTAAACgc                       <  2:1935747/140‑2 (MQ=255)                                                                                                                       tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                      >  1:360138/1‑140 (MQ=255)                                                                                                                       tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                      >  1:368097/1‑140 (MQ=255)                                                                                                                          gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                   >  2:1247751/1‑140 (MQ=255)                                                                                                                          gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                   >  1:1707465/1‑140 (MQ=255)                                                                                                                          gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                   >  2:310038/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg                                  >  2:883834/1‑124 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  2:691854/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  1:1241764/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                  >  1:183079/1‑140 (MQ=255)                                                                                                                           ttCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAg                                  <  1:883834/124‑1 (MQ=255)                                                                                                                            tCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCAc                 >  1:1347969/1‑140 (MQ=255)                                                                                                                              aTGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg               >  2:2046700/1‑140 (MQ=255)                                                                                                                                gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc             >  1:374211/1‑140 (MQ=255)                                                                                                                                gCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc             >  1:1935747/1‑140 (MQ=255)                                                                                                                                     gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc        >  1:2401771/1‑140 (MQ=255)                                                                                                                                     gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc        >  1:705915/1‑140 (MQ=255)                                                                                                                                      aaaacCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCACGGTGTTAAACGATGCCACGGGCGAGACt       <  2:2247246/140‑1 (MQ=255)                                                                                                                                      aaaacCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACt       <  1:310038/140‑1 (MQ=255)                                                                                                                                       aaaaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACtt      <  2:705915/140‑1 (MQ=255)                                                                                                                                         aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc    <  1:576027/140‑1 (MQ=255)                                                                                                                                           cTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGt  >  2:885934/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                           ACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGT  >  NC_000913/3556068‑3556345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑