BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,131,218	C→A	A285S (GCT→TCT)	wcaC ←	putative colanic acid biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,131,218	0	C	A	100.0%	379.2 / NA	106	A285S (GCT→TCT)	wcaC	putative colanic acid biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (58/48); total (58/48)

Only 100 of 107 total aligned reads displayed.

CAATCGATAGCGCCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCTTGCCCT  >  NC_000913/2131083‑2131355

cAATCGATAGCGCCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTg                                                                                                                                       <  1:2280170/140‑1 (MQ=255)

 aaTCGATAGCGCCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGa                                                                                                                                      <  1:1073399/140‑1 (MQ=255)

  aTCGATAGCGCCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGaa                                                                                                                                     >  2:1809584/1‑140 (MQ=255)

         gcgcCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCgag                                                                                                                              <  1:2149793/140‑1 (MQ=255)

         gcgcCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCgag                                                                                                                              <  2:456981/140‑1 (MQ=255)

          cgcCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCgaga                                                                                                                             >  2:40750/1‑140 (MQ=255)

           gcCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGaa                                                                                                                            <  1:1809584/140‑1 (MQ=255)

           gcCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGaa                                                                                                                            <  2:1914599/140‑1 (MQ=255)

            ccTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGa                                                                                                                                      >  1:1406915/1‑129 (MQ=255)

            ccTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGa                                                                                                                                      <  2:1406915/129‑1 (MQ=255)

               cacacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGa                                                                                                                                      >  2:1743221/1‑126 (MQ=255)

               cacacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGa                                                                                                                                      <  1:1743221/126‑1 (MQ=255)

                acacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCGGAGGTGAACGGCGAGAACTTAc                                                                                                                       <  1:904271/140‑1 (MQ=255)

                acacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTAc                                                                                                                       <  1:40750/140‑1 (MQ=255)

                acacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTAc                                                                                                                       <  1:1598581/140‑1 (MQ=255)

                 cacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTAcc                                                                                                                      >  1:2104395/1‑140 (MQ=255)

                 cacaAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTAcc                                                                                                                      >  1:105722/1‑140 (MQ=255)

                      aaTCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAgg                                                                                                                 <  2:1486531/140‑1 (MQ=255)

                         cAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTAt                                                                                                              <  2:2175908/140‑1 (MQ=255)

                          aGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATg                                                                                                             <  1:1056346/140‑1 (MQ=255)

                            cGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCa                                                                                                           >  1:1984342/1‑140 (MQ=255)

                              gggagctATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGt                                                                                                         >  2:1999447/7‑140 (MQ=255)

                              ggTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGt                                                                                                         >  1:170140/1‑140 (MQ=255)

                              ggTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGt                                                                                                         >  2:537950/1‑140 (MQ=255)

                              ggTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGt                                                                                                         >  1:2055081/1‑140 (MQ=255)

                                  gTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCaa                                                                                                     <  2:757495/140‑1 (MQ=255)

                                         aCGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTc                                                                                                       >  2:2432824/1‑131 (MQ=255)

                                         aCGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTc                                                                                                       <  1:2432824/131‑1 (MQ=255)

                                          cgcgAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCg                                                                                             >  2:1550174/1‑140 (MQ=255)

                                          cgcgAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCg                                                                                             >  1:790406/1‑140 (MQ=255)

                                           gcgAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGc                                                                                            >  2:2155125/1‑140 (MQ=255)

                                            cgAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGcc                                                                                           <  1:1233871/140‑1 (MQ=255)

                                               gaACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAg                                                                                        <  1:2155125/140‑1 (MQ=255)

                                                     aaTACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCcat                                                                                  >  1:510393/1‑140 (MQ=255)

                                                          cAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCAtct                                                                             <  2:1984342/140‑1 (MQ=255)

                                                          cAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCAtct                                                                             >  2:2009683/1‑140 (MQ=255)

                                                            gcgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTcg                                                                           >  2:1032210/1‑140 (MQ=255)

                                                            gcgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGCCGCCCAGCGCCATCATCTcg                                                                           <  1:1378790/140‑1 (MQ=255)

                                                             cgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCcatca                                                                                <  2:1796477/134‑1 (MQ=255)

                                                             cgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCcatca                                                                                >  1:1796477/1‑134 (MQ=255)

                                                             cgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTcgc                                                                          >  1:2357571/1‑140 (MQ=255)

                                                             cgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTcgc                                                                          <  1:1819463/140‑1 (MQ=255)

                                                             cgcATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTcgc                                                                          >  1:1522532/1‑140 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCcatca                                                                                <  1:1796589/132‑1 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCatcatc                                                                              >  2:1953961/1‑134 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCatcatc                                                                              >  1:426501/1‑134 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCatcatc                                                                              <  1:1953961/134‑1 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCatcatc                                                                              <  2:426501/134‑1 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTcgcg                                                                         <  1:1383399/139‑1 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTcgcg                                                                         >  2:1383399/1‑139 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGt                                                                        >  1:1671862/1‑140 (MQ=255)

                                                               catccatcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGt                                                                        >  2:1374437/1‑140 (MQ=255)

                                                                    atcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa                                                                       <  1:1768381/136‑1 (MQ=255)

                                                                    atcTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa                                                                       >  2:1768381/1‑136 (MQ=255)

                                                                       tGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTg                                                                <  1:1374437/140‑1 (MQ=255)

                                                                       tGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTg                                                                <  2:510393/140‑1 (MQ=255)

                                                                         aTTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCcatca                                                                                <  1:585725/122‑1 (MQ=255)

                                                                         aTTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCcatca                                                                                >  2:585725/1‑122 (MQ=255)

                                                                               cgcgcTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAg                                                        >  2:2332351/1‑140 (MQ=255)

                                                                               cgcgcTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAg                                                        >  1:2286061/1‑140 (MQ=255)

                                                                               cgcgcTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCAAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAg                                                        >  1:2204597/1‑140 (MQ=255)

                                                                                gcgcTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGt                                                       >  2:2213709/1‑140 (MQ=255)

                                                                                       tcaGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGATGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcg                                                <  1:1743218/140‑1 (MQ=255)

                                                                                          gCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcg                                             <  1:1013610/140‑1 (MQ=255)

                                                                                          gCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcg                                             <  2:1671862/140‑1 (MQ=255)

                                                                                           cTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt                                            <  2:1522532/140‑1 (MQ=255)

                                                                                           cTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt                                            >  1:1179323/1‑140 (MQ=255)

                                                                                              aCGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtc                                              <  1:1751686/135‑1 (MQ=255)

                                                                                              aCGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtc                                              >  2:1751686/1‑135 (MQ=255)

                                                                                                gTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGc                                       >  1:678739/1‑140 (MQ=255)

                                                                                                gTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGc                                       >  1:418623/1‑140 (MQ=255)

                                                                                                    gTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt                                   >  2:1001036/1‑140 (MQ=255)

                                                                                                     tCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATc                                  >  2:520353/1‑140 (MQ=255)

                                                                                                     tCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCACTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGc                                                  >  2:1173173/1‑124 (MQ=255)

                                                                                                     tCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCACTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGc                                                  <  1:1173173/124‑1 (MQ=255)

                                                                                                        gTTTCAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATg                               <  2:678739/140‑1 (MQ=255)

                                                                                                            cAAAGCCGTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGcc                           >  2:2152824/1‑140 (MQ=255)

                                                                                                                   gTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACcg                    >  1:101616/1‑140 (MQ=255)

                                                                                                                   gTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACcg                    >  1:1588460/1‑140 (MQ=255)

                                                                                                                   gTGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACcg                    >  2:2275262/1‑140 (MQ=255)

                                                                                                                    tGATTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACcgc                   >  1:67205/1‑140 (MQ=255)

                                                                                                                      aTTAACCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCACAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGa                 >  2:1384457/1‑140 (MQ=255)

                                                                                                                          aCCACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGAtttt             >  2:1602096/1‑140 (MQ=255)

                                                                                                                           ccACGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTc            >  1:1434016/1‑140 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:2275262/140‑1 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:1001036/140‑1 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:2009683/140‑1 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:2213709/140‑1 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:2433631/140‑1 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         >  2:2012093/1‑140 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:537950/140‑1 (MQ=255)

                                                                                                                              cGTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         >  2:1873997/1‑140 (MQ=255)

                                                                                                                               gTTGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATTTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCt        >  2:828546/1‑140 (MQ=255)

                                                                                                                                ttGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCtt       <  1:2012093/140‑1 (MQ=255)

                                                                                                                                ttGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCtt       >  2:732884/1‑140 (MQ=255)

                                                                                                                                 tGCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCTTg      >  1:350331/1‑140 (MQ=255)

                                                                                                                                  gCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         >  2:364786/1‑136 (MQ=255)

                                                                                                                                  gCCAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGc         <  1:364786/136‑1 (MQ=255)

                                                                                                                                   ccAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCTTGcc    <  2:1588460/140‑1 (MQ=255)

                                                                                                                                    cAGAGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGC‑TGCCCt  >  2:478587/1‑140 (MQ=255)

CAATCGATAGCGCCTCACACAAAATCAGCGGGTAGTTATCGACGCGAGAACTGAATACCAGCGCATCCATCTGATTGAGCGCGCTCATCAGCTTACGTTTGTCGGTTTCAAAGCCGTGATTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGATCATGCGCCACCACCGCGATTTTCGGCTTGCCCT  >  NC_000913/2131083‑2131355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`