BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,131,320	G→T	G496C (GGT→TGT)	metL →	fused aspartate kinase/homoserine dehydrogenase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,131,320	0	G	T	100.0%	453.0 / NA	127	G496C (GGT→TGT)	metL	fused aspartate kinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (65/62); total (65/62)

Only 100 of 127 total aligned reads displayed.

AGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGA  >  NC_000913/4131183‑4131456

aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATgt                                                                                                                                        >  1:538762/1‑140 (MQ=255)

aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATgt                                                                                                                                        >  1:1760713/1‑140 (MQ=255)

       cTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGtggtgg                                                                                                                                 <  2:376821/140‑1 (MQ=255)

                aGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAgccgcc                                                                                                                        >  2:260422/1‑140 (MQ=255)

                  aaaaaCGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCcgccgc                                                                                                                      <  2:1533946/140‑1 (MQ=255)

                   aaaaCGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCa                                                                                                                     <  2:1109988/140‑1 (MQ=255)

                    aaaCGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAg                                                                                                                    <  1:734269/140‑1 (MQ=255)

                     aaCGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGc                                                                                                                   <  2:1608581/140‑1 (MQ=255)

                     aaCGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGc                                                                                                                   <  1:369641/140‑1 (MQ=255)

                        gCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTg                                                                                                                <  2:538762/140‑1 (MQ=255)

                         cATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGt                                                                                                               <  1:1772341/140‑1 (MQ=255)

                          aTCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGtt                                                                                                              <  1:1306420/140‑1 (MQ=255)

                                   gTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGa                                                                                                     <  1:1389669/140‑1 (MQ=255)

                                        gTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGc                                                                                                >  1:1284252/1‑140 (MQ=255)

                                         ttCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCt                                                                                               >  2:1599719/1‑140 (MQ=255)

                                         ttCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCt                                                                                               >  2:1644290/1‑140 (MQ=255)

                                         ttCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCt                                                                                               >  1:286783/1‑140 (MQ=255)

                                             gTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTg                                                                                                             <  2:1124986/122‑1 (MQ=255)

                                             gTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTg                                                                                                             >  1:1124986/1‑122 (MQ=255)

                                             gTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGAc                                                                                           >  1:706255/1‑140 (MQ=255)

                                             gTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGAc                                                                                           >  2:966159/1‑140 (MQ=255)

                                              tAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACg                                                                                          >  1:1870852/1‑140 (MQ=255)

                                              tAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACg                                                                                          >  2:67517/1‑140 (MQ=255)

                                              tAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACg                                                                                          >  2:691704/1‑140 (MQ=255)

                                              tAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTCGAGCTATGACGGGCTGGACg                                                                                          >  2:669260/1‑140 (MQ=255)

                                                aGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGcc                                                                                        >  2:312294/1‑140 (MQ=255)

                                                       tatCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCcgcg                                                                                 <  1:1599719/140‑1 (MQ=255)

                                                       tatCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCcgcg                                                                                 <  1:67517/140‑1 (MQ=255)

                                                         tCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCcgcgcg                                                                               <  1:451392/140‑1 (MQ=255)

                                                         tCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCcgcgcg                                                                               <  1:1612585/140‑1 (MQ=255)

                                                          cGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGt                                                                              <  2:1776510/140‑1 (MQ=255)

                                                            gTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTa                                                                            >  1:1765435/1‑140 (MQ=255)

                                                            gTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTa                                                                            >  2:609566/1‑140 (MQ=255)

                                                            gTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTATGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTa                                                                            >  2:1788897/1‑140 (MQ=255)

                                                               cccGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGcc                                                                         <  2:417091/140‑1 (MQ=255)

                                                                    tGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCcttctt                                                                    <  2:1765435/140‑1 (MQ=255)

                                                                    tGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCcttctt                                                                    <  1:609566/140‑1 (MQ=255)

                                                                       cTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCcg                                                                                   <  1:392716/122‑1 (MQ=255)

                                                                       cTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCcg                                                                                   >  2:392716/1‑122 (MQ=255)

                                                                               gTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAg                                                         <  1:986193/140‑1 (MQ=255)

                                                                               gTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAg                                                         <  1:691704/140‑1 (MQ=255)

                                                                               gTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAg                                                         <  1:1802551/140‑1 (MQ=255)

                                                                               gTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAg                                                         <  1:312294/140‑1 (MQ=255)

                                                                                ttCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGc                                                        >  2:2017109/1‑140 (MQ=255)

                                                                                ttCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGc                                                        >  1:547676/1‑140 (MQ=255)

                                                                                ttCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGc                                                        >  1:1951342/1‑140 (MQ=255)

                                                                                   gCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGt                                                     <  2:823401/140‑1 (MQ=255)

                                                                                     ccGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTg                                                   >  1:2048501/1‑140 (MQ=255)

                                                                                     ccGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTg                                                   >  1:906838/1‑140 (MQ=255)

                                                                                     ccGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTg                                                   >  2:66981/1‑140 (MQ=255)

                                                                                      cGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGa                                                  <  2:228231/140‑1 (MQ=255)

                                                                                      cGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGa                                                  <  2:1951342/140‑1 (MQ=255)

                                                                                       gTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGTTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAg                                                 <  2:712650/140‑1 (MQ=255)

                                                                                       gTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAg                                                 <  1:2017109/140‑1 (MQ=255)

                                                                                        tGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGc                                                <  1:463584/140‑1 (MQ=255)

                                                                                          agcagagcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAg                                              >  1:852774/1‑140 (MQ=255)

                                                                                          agcagagcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAg                                              >  2:811739/1‑140 (MQ=255)

                                                                                               agcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGc                                                        <  1:839874/125‑1 (MQ=255)

                                                                                               agcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGc                                                        >  2:839874/1‑125 (MQ=255)

                                                                                               agcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGa                                         >  1:600552/1‑140 (MQ=255)

                                                                                               agcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGa                                         >  1:905449/1‑140 (MQ=255)

                                                                                                gcaCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGaa                                        <  2:1870852/140‑1 (MQ=255)

                                                                                                 caCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAag                                       >  1:1126272/1‑140 (MQ=255)

                                                                                                 caCGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAag                                       >  1:123494/1‑140 (MQ=255)

                                                                                                    gCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGc                                                >  2:1853370/1‑128 (MQ=255)

                                                                                                    gCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGc                                                <  1:1853370/128‑1 (MQ=255)

                                                                                                    gCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGt                                    <  1:966159/140‑1 (MQ=255)

                                                                                                    gCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGt                                    >  2:529148/1‑140 (MQ=255)

                                                                                                    gCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGt                                    <  2:123494/140‑1 (MQ=255)

                                                                                                      tttCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCg                                  >  1:1866047/1‑140 (MQ=255)

                                                                                                      tttCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCg                                  >  1:65122/1‑140 (MQ=255)

                                                                                                       ttCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCgt                                 >  1:337124/1‑140 (MQ=255)

                                                                                                        tCGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCgtt                                <  2:2040833/140‑1 (MQ=255)

                                                                                                         cGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCgttg                               >  2:1735050/1‑140 (MQ=255)

                                                                                                         cGGCACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCgttg                               >  2:919917/1‑140 (MQ=255)

                                                                                                            cACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAaga                                      <  2:1094042/130‑1 (MQ=255)

                                                                                                            cACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAaga                                      >  1:1094042/1‑130 (MQ=255)

                                                                                                            cACGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTc                            <  2:1126272/140‑1 (MQ=255)

                                                                                                              cGTACCGGCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCt                          <  2:2047433/140‑1 (MQ=255)

                                                                                                                     gCTTTGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATg                   <  1:1560664/140‑1 (MQ=255)

                                                                                                                         tGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTTTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATgcgcg               <  2:1451329/140‑1 (MQ=255)

                                                                                                                         tGAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATgcgcg               <  2:1866047/140‑1 (MQ=255)

                                                                                                                          gAGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATgcgcgc              >  2:819030/1‑140 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             <  1:1986154/140‑1 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             <  1:1644290/140‑1 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             <  2:547676/140‑1 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             >  2:455255/1‑140 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             <  1:260422/140‑1 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             >  2:1465473/1‑140 (MQ=255)

                                                                                                                           aGTTTGTGCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTCGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             <  1:669260/140‑1 (MQ=255)

                                                                                                                           aGTTTGTGCGGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGcc             <  1:812385/140‑1 (MQ=255)

                                                                                                                               tgtgCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATc         >  2:688492/1‑140 (MQ=255)

                                                                                                                                 tgCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCg       <  2:306071/140‑1 (MQ=255)

                                                                                                                                 tgCTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCg       >  2:908407/1‑140 (MQ=255)

                                                                                                                                   cTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTa     <  1:455255/140‑1 (MQ=255)

                                                                                                                                   cTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTa     <  2:905449/140‑1 (MQ=255)

                                                                                                                                   cTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTa     <  1:66981/140‑1 (MQ=255)

                                                                                                                                   cTGGCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTa     <  1:1107043/140‑1 (MQ=255)

                                                                                                                                     ggCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTatg   >  2:1452466/1‑140 (MQ=255)

                                                                                                                                      gCATGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTatga  <  1:201690/140‑1 (MQ=255)

AGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGTGCTGGCAGGTGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTATGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCGGTTGAGCAGGATGAAGAGTCGTTGTTCCTGTGGATGCGCGCCCATCCGTATGA  >  NC_000913/4131183‑4131456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`