Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	595,903	C→A	L102I (CTC→ATC)	cusC →	copper/silver export system outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	595,903	0	C	A	100.0%	141.8 / NA	42	L102I (CTC→ATC)	cusC	copper/silver export system outer membrane channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/18);  total (24/18)

GATAATCAGGTGAAGACGCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGA  >  NC_000913/595777‑596033                                                                                                                               |                                                                                                                                   gATAATCAGGTGAAGACGCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAggg                                                                                                                       >  1:1815935/1‑140 (MQ=255)        aGGTGAAGACGCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGggcagcggc                                                                                                                <  2:1533784/140‑1 (MQ=255)         ggTGAAGACGCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGggcagcggca                                                                                                               >  1:35991/1‑140 (MQ=255)                aCGCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAg                                                                                                        <  2:668079/140‑1 (MQ=255)                  gCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCg                                                                                                      >  1:1110866/1‑140 (MQ=255)                   cTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCgg                                                                                                     <  2:684242/140‑1 (MQ=255)                    tGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGt                                                                                                    >  2:974061/1‑140 (MQ=255)                    tGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGt                                                                                                    <  2:880268/140‑1 (MQ=255)                     gATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTa                                                                                                   <  2:1815935/140‑1 (MQ=255)                        tAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATc                                                                                                <  2:509118/140‑1 (MQ=255)                        tAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATc                                                                                                <  2:951645/140‑1 (MQ=255)                          gCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCtt                                                                                              <  2:591824/140‑1 (MQ=255)                          gCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCtt                                                                                              <  2:349084/140‑1 (MQ=255)                           cGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTa                                                                                             <  1:974061/140‑1 (MQ=255)                                cgcTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAggg                                                                                        >  1:369857/1‑140 (MQ=255)                                          taatCGGGATTTGCGCATGGCGACGTTGAAAGTGCAGGAGGCACGGGCGCAATACCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCa                                                                              <  2:1246619/140‑1 (MQ=255)                                             tCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGa                                                                           >  2:372346/1‑140 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGaca                                                                         >  2:870859/1‑140 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGaca                                                                         >  1:732943/1‑140 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGaca                                                                         >  1:815839/1‑140 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGaca                                                                         >  2:1084021/1‑140 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGaca                                                                         >  1:1914344/1‑140 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCCACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGc                                                                                >  1:2064195/1‑133 (MQ=255)                                               gggATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCCACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGc                                                                                <  2:2064195/133‑1 (MQ=255)                                                  aTTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACgg                                                                      <  1:1081503/140‑1 (MQ=255)                                                   tttGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACggg                                                                     <  2:815839/140‑1 (MQ=255)                                                            ggCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGa                                                            >  2:1559042/1‑140 (MQ=255)                                                            ggCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGa                                                            <  1:564639/140‑1 (MQ=255)                                                                              ggaggCCGGGGCGAAATATCGTCTGACGGATGCCGGCCGCTCCCCACAGAACAATGGGGGGGGGGGCGGCGGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCt                                          <  2:732943/140‑1 (MQ=255)                                                                                   cACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGAc                                     <  1:372346/140‑1 (MQ=255)                                                                                    ccGGGCGCAATATCGTCTGGCCGAGGCCGACGGCTACCCAGAGAGCAATGGCGAGGGCAGCGGCAGTTGGAGCGGTAATCTTAAGGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGAcc                                    <  2:71999/139‑1 (MQ=255)                                                                                    aCGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGAcc                                    <  2:1914344/140‑1 (MQ=255)                                                                                    aCGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGAcc                                    >  1:71999/1‑140 (MQ=255)                                                                                     cGGGCGCAATATCGTCTGCCCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCt                                   <  1:1415101/140‑1 (MQ=255)                                                                                       ggCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCg                                 >  2:1129158/1‑140 (MQ=255)                                                                                       ggCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCg                                 <  1:1129158/140‑1 (MQ=255)                                                                                             atatCGTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGAttttt                           >  1:437210/1‑140 (MQ=255)                                                                                                  gTCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGt                      >  2:911655/1‑140 (MQ=255)                                                                                                   tCTGACCGATGCCGTCCGCTCCCCACAGATCAATGGCGAGGCCGGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTc                     <  2:452020/140‑1 (MQ=255)                                                                                                   tCTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTc                     >  2:1257904/1‑140 (MQ=255)                                                                                                    cTGACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCg                    <  2:2017915/140‑1 (MQ=255)                                                                                                      gACCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCt                  >  1:1075137/1‑140 (MQ=255)                                                                                                       aCCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCtt                 >  2:758360/1‑140 (MQ=255)                                                                                                       aCCGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCtt                 >  2:1051088/1‑140 (MQ=255)                                                                                                        ccGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTa                >  1:422848/1‑140 (MQ=255)                                                                                                        ccGATGCCGACCGCTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTa                >  1:869875/1‑140 (MQ=255)                                                                                                                     cTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAg     >  1:922304/1‑138 (MQ=255)                                                                                                                     cTACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAg     <  2:922304/138‑1 (MQ=255)                                                                                                                      tACCCACAGATCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGGATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGa  >  2:18596/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                   GATAATCAGGTGAAGACGCTGATTAGCGAGGCGCTGGTGAATAATCGGGATTTGCGCATGGCGACGCTGAAAGTGCAGGAGGCACGGGCGCAATATCGTCTGACCGATGCCGACCGCTACCCACAGCTCAATGGCGAGGGCAGCGGCAGCTGGAGCGGTAATCTTAAAGGGAATACCGCCACGACACGGGAGTTTTCGACCGGCCTTAACGCCAGCTTTGACCTCGATTTTTTCGGTCGCTTAAAGAACATGAGCGA  >  NC_000913/595777‑596033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑