Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,850,380	C→A	R19L (CGC→CTC)	hycA ←	regulator of the transcriptional regulator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,850,380	0	C	A	100.0%	247.2 / NA	71	R19L (CGC→CTC)	hycA	regulator of the transcriptional regulator FhlA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/46);  total (25/46)

TGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGA  >  NC_000913/2850246‑2850512                                                                                                                                       |                                                                                                                                      tGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGagacga                                                                                                                                  <  1:229962/140‑1 (MQ=255) tGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGagacga                                                                                                                                  <  2:231941/140‑1 (MQ=255)   cGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATg                                                                                                                                <  2:1586472/140‑1 (MQ=255)    gAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGc                                                                                                                               >  2:1009017/1‑140 (MQ=255)           ttCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCtgtg                                                                                                                        <  1:456004/140‑1 (MQ=255)                aaaGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATg                                                                                                                   >  1:1546683/1‑140 (MQ=255)                     aCGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCg                                                                                                                             >  1:1621283/1‑125 (MQ=255)                     aCGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCg                                                                                                                             <  2:1621283/125‑1 (MQ=255)                         aaCAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCt                                                                                                          <  2:456097/140‑1 (MQ=255)                             gagTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTtct                                                                                                      <  2:459111/140‑1 (MQ=255)                              agTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTtctc                                                                                                     >  1:1663758/1‑140 (MQ=255)                              agTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTtctc                                                                                                     <  2:1536959/140‑1 (MQ=255)                                    ttGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTAt                                                                                               <  2:1343132/140‑1 (MQ=255)                                    ttGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTAt                                                                                               <  2:1663758/140‑1 (MQ=255)                                      gTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTAttt                                                                                             <  2:260508/140‑1 (MQ=255)                                          ggCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCa                                                                                         >  2:1133483/1‑140 (MQ=255)                                          ggCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCa                                                                                         <  2:1392814/140‑1 (MQ=255)                                          ggCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCa                                                                                         <  1:1009017/140‑1 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCttt                                                                                                        >  1:1114611/1‑123 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCttt                                                                                                        <  2:1114611/123‑1 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCttt                                                                                                        >  1:713799/1‑123 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCttt                                                                                                        <  2:713799/123‑1 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCaaa                                                                                       <  2:681835/140‑1 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCaaa                                                                                       <  1:279201/140‑1 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCaaa                                                                                       <  2:1448994/140‑1 (MQ=255)                                            cgcgcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCaaa                                                                                       <  1:212927/140‑1 (MQ=255)                                               gcAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAg                                                                                    <  2:882999/140‑1 (MQ=255)                                                  gCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCa                                                                                 <  1:394075/140‑1 (MQ=255)                                                    tCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCAtt                                                                               <  1:561945/140‑1 (MQ=255)                                                    tCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCAtt                                                                               <  2:902374/140‑1 (MQ=255)                                                          cGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAgg                                                                         <  1:423140/140‑1 (MQ=255)                                                            tGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGtt                                                                       <  2:942313/140‑1 (MQ=255)                                                             gATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTa                                                                      >  2:59018/1‑140 (MQ=255)                                                              aTGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTAc                                                                     <  2:871959/140‑1 (MQ=255)                                                              aTGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTAc                                                                     <  2:1284432/140‑1 (MQ=255)                                                                         cTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACag                                                          >  1:1452338/1‑140 (MQ=255)                                                                         cTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCAGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACag                                                          >  2:1482528/1‑140 (MQ=255)                                                                          tttCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACaga                                                         <  1:59018/140‑1 (MQ=255)                                                                              gATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATg                                                     <  1:993535/140‑1 (MQ=255)                                                                                    gTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCt                                               >  2:1447593/1‑140 (MQ=255)                                                                                      gATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCATGTTACCCGTTTAACAGAGATGCCAGCttt                                             >  2:846961/1‑140 (MQ=255)                                                                                      gATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCttt                                             >  2:520216/1‑140 (MQ=255)                                                                                       aTCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                            <  1:1472865/140‑1 (MQ=255)                                                                                       aTCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                            <  1:1447593/140‑1 (MQ=255)                                                                                         ccccTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAg                                          >  1:1315733/1‑140 (MQ=255)                                                                                              gAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑ttt                                    >  1:1423925/1‑140 (MQ=255)                                                                                              gAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑ttt                                    >  1:1254213/1‑140 (MQ=255)                                                                                                    gCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTtgtg                              <  2:316038/140‑1 (MQ=255)                                                                                                    gCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTtgtg                              >  1:394117/1‑140 (MQ=255)                                                                                                    gCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTtgtg                              >  2:1637225/1‑140 (MQ=255)                                                                                                     cGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGc                             <  1:1482528/140‑1 (MQ=255)                                                                                                     cGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGc                             <  1:1181756/140‑1 (MQ=255)                                                                                                     cGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGc                             >  1:1666273/1‑140 (MQ=255)                                                                                                     cGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGc                             <  2:1254213/140‑1 (MQ=255)                                                                                                      gAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGcc                            <  1:1133483/140‑1 (MQ=255)                                                                                                      gAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGcc                            <  2:1425256/140‑1 (MQ=255)                                                                                                      gAATTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGcc                            >  2:354371/1‑140 (MQ=255)                                                                                                       aaTTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCa                           <  2:762179/140‑1 (MQ=255)                                                                                                        aTTGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCTGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTTGTGCCa                           <  2:1315733/140‑1 (MQ=255)                                                                                                         ttGCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAAc                         >  1:266832/1‑140 (MQ=255)                                                                                                           gCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACtt                       <  2:109282/140‑1 (MQ=255)                                                                                                           gCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACtt                       <  2:394117/140‑1 (MQ=255)                                                                                                           gCAGTAGATGTGCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACtt                       <  1:1637225/140‑1 (MQ=255)                                                                                                                    gtgCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGc                             <  1:141246/125‑1 (MQ=255)                                                                                                                    gtgCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGc                             >  2:141246/1‑125 (MQ=255)                                                                                                                    gtgCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTAt              >  1:141279/1‑140 (MQ=255)                                                                                                                    gtgCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTAt              <  2:1423925/140‑1 (MQ=255)                                                                                                                    gtgCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTAt              <  2:1452338/140‑1 (MQ=255)                                                                                                                      gCCACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTATTg            <  2:141279/140‑1 (MQ=255)                                                                                                                       ccACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGcc                            >  1:1036838/1‑123 (MQ=255)                                                                                                                       ccACTGGTCCTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGcc                            <  2:1036838/123‑1 (MQ=255)                                                                                                                               ccTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTATTGTTATTAAag   >  2:1232860/1‑140 (MQ=255)                                                                                                                                cTGTAGGAGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTATTGTTATTAAaga  <  2:266832/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      TGCGAAAATGTTCAAAAAGGACGAAACAGAGTTCTTTGTCCGGCGCGCAGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCA‑TTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGA  >  NC_000913/2850246‑2850512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑