Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	415,159	C→A	E199* (GAG→TAG)	sbcC ←	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	415,159	0	C	A	100.0%	202.9 / NA	58	E199* (GAG→TAG)	sbcC	ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/28);  total (30/28)

GTTGTTCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCG  >  NC_000913/415024‑415293                                                                                                                                        |                                                                                                                                       gttgttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTAtgtg                                                                                                                                    >  2:1674100/1‑140 (MQ=255) gttgttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTAtgtg                                                                                                                                    >  1:1492471/1‑140 (MQ=255)  ttgttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGc                                                                                                                                   <  1:1057561/140‑1 (MQ=255)     ttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCggg                                                                                                                                <  2:454131/140‑1 (MQ=255)     ttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCggg                                                                                                                                <  2:445539/140‑1 (MQ=255)     ttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCggg                                                                                                                                <  2:433083/140‑1 (MQ=255)     ttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCggg                                                                                                                                <  2:1591646/140‑1 (MQ=255)     ttCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCggg                                                                                                                                >  1:1542785/1‑140 (MQ=255)              ctgcGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTtgt                                                                                                                       >  1:624010/1‑140 (MQ=255)               tgcGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTtgtg                                                                                                                      >  1:421580/1‑140 (MQ=255)                gcgcGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGtgc                                                                                                                     <  2:421580/140‑1 (MQ=255)                 cgcgGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGtgct                                                                                                                    >  2:903183/1‑140 (MQ=255)                  gcgGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGtgctg                                                                                                                   <  2:505995/140‑1 (MQ=255)                             cTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCa                                                                                                        <  2:934900/140‑1 (MQ=255)                                tttttCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCg                                                                                                     <  1:177621/140‑1 (MQ=255)                                tttttCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCg                                                                                                     >  2:660186/1‑140 (MQ=255)                                 ttttCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGc                                                                                                    <  1:1674100/140‑1 (MQ=255)                                  tttCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGcc                                                                                                   >  1:492821/1‑140 (MQ=255)                                         gTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGAttt                                                                                            <  2:624010/140‑1 (MQ=255)                                                    ccTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGAtt                                                                                 >  1:1135872/1‑140 (MQ=255)                                                     cTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGAttt                                                                                <  1:903183/140‑1 (MQ=255)                                                      tGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTc                                                                               >  1:1287261/1‑140 (MQ=255)                                                       gCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGc                                                                                          >  2:607936/1‑128 (MQ=255)                                                       gCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGc                                                                                          <  1:607936/128‑1 (MQ=255)                                                       gCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCa                                                                              >  1:1506016/1‑140 (MQ=255)                                                        cAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAg                                                                             >  2:381839/1‑140 (MQ=255)                                                         aaaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGt                                                                            <  2:492821/140‑1 (MQ=255)                                                          aaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg                                                                           <  1:1315918/140‑1 (MQ=255)                                                          aaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg                                                                           <  2:1287261/140‑1 (MQ=255)                                                          aaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg                                                                           >  2:1315918/1‑140 (MQ=255)                                                          aaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg                                                                           <  1:1351768/140‑1 (MQ=255)                                                          aaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg                                                                           <  1:381839/140‑1 (MQ=255)                                                          aaCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTg                                                                           <  2:894829/140‑1 (MQ=255)                                                                  gTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTaa                                                                   >  1:853926/1‑140 (MQ=255)                                                                      gCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAActcc                                                               <  2:1222769/140‑1 (MQ=255)                                                                           tGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAg                                                          >  2:1114893/1‑140 (MQ=255)                                                                                  gTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTcc                                                   >  2:1427692/1‑140 (MQ=255)                                                                                   ttCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcg                                                  >  1:1120680/1‑140 (MQ=255)                                                                                    tCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgc                                                 >  2:1070002/1‑140 (MQ=255)                                                                                     ccGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcg                                                <  1:1036290/140‑1 (MQ=255)                                                                                     ccGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcg                                                <  1:660186/140‑1 (MQ=255)                                                                                      cGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCcgcgc                                               <  1:1222843/140‑1 (MQ=255)                                                                                        gCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGt                                             >  2:1150619/1‑140 (MQ=255)                                                                                        gCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGt                                             >  2:675621/1‑140 (MQ=255)                                                                                        gCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGt                                             >  1:1228296/1‑140 (MQ=255)                                                                                         cGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGtt                                            >  1:69028/1‑140 (MQ=255)                                                                                             aGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCttt                                        >  2:283054/1‑140 (MQ=255)                                                                                             aGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCttt                                        <  1:283054/140‑1 (MQ=255)                                                                                                  cGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGtt                                   <  1:1427692/140‑1 (MQ=255)                                                                                                      aCGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGc                               >  1:1451990/1‑140 (MQ=255)                                                                                                            cTGGCCTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAg                         >  2:1283930/1‑140 (MQ=255)                                                                                                                 cTGCGCTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAgg                    >  1:161704/1‑140 (MQ=255)                                                                                                                   gcgcTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGgca                  <  1:1283930/140‑1 (MQ=255)                                                                                                                    cgcTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGgcag                 >  2:1137742/1‑140 (MQ=255)                                                                                                                    cgcTTGCAGCTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGgcag                 >  1:1549995/1‑140 (MQ=255)                                                                                                                             cTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGcc        <  2:1506016/140‑1 (MQ=255)                                                                                                                             cTTCTCCAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGcc        <  2:1135872/140‑1 (MQ=255)                                                                                                                                   cAGCTATGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCg  >  1:334503/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GTTGTTCTTGCTGCTGCGCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGATTTGCCCGTAGATTTCAGTGCCGGTTAACTCCTCGAGCAATTCCGCGCGTTCTTTGGGTTTGGCATTCAGGAAGGCAGCAAATTGCCCCTGCG  >  NC_000913/415024‑415293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑