Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,343	G→T	T270T (ACC→ACA)	btuC ←	vitamin B12 ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,343	0	G	T	100.0%	275.8 / NA	78	T270T (ACC→ACA)	btuC	vitamin B12 ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (44/34);  total (44/34)

ACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTAA  >  NC_000913/1794206‑1794477                                                                                                                                          |                                                                                                                                       acCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGt                                                                                                                                      >  2:1607502/1‑140 (MQ=255)    ggCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTaa                                                                                                                                   >  1:1767751/1‑140 (MQ=255)     gcgcACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTaaa                                                                                                                                  >  2:2113784/1‑140 (MQ=255)     gcgcACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTaaa                                                                                                                                  >  1:2023996/1‑140 (MQ=255)     gcgcACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGAAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTaaa                                                                                                                                  >  2:572911/1‑140 (MQ=255)      cgcACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAAc                                                                                                                                 >  2:1202448/1‑140 (MQ=255)         aCCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcac                                                                                                                              >  2:1741984/1‑140 (MQ=255)          ccTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcaca                                                                                                                             >  2:552705/1‑140 (MQ=255)          ccTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcaca                                                                                                                             <  2:2180957/140‑1 (MQ=255)          ccTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcaca                                                                                                                             <  1:2110712/140‑1 (MQ=255)          ccTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcaca                                                                                                                             <  1:1439422/140‑1 (MQ=255)           cTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcacac                                                                                                                            >  1:1865801/1‑140 (MQ=255)           cTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACcacac                                                                                                                            <  2:1865801/140‑1 (MQ=255)                gTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCg                                                                                                                       <  1:1741984/140‑1 (MQ=255)                gTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCAGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCg                                                                                                                       <  1:1315020/140‑1 (MQ=255)                  tGCGGTGACCTCGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGga                                                                                                                     <  2:1603738/140‑1 (MQ=255)                  tGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGga                                                                                                                     >  2:1405801/1‑140 (MQ=255)                  tGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGga                                                                                                                     <  1:1405801/140‑1 (MQ=255)                   gCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGgag                                                                                                                    >  1:1835456/1‑140 (MQ=255)                   gCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGgag                                                                                                                    <  1:1607502/140‑1 (MQ=255)                   gCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGgag                                                                                                                    >  2:1386957/1‑140 (MQ=255)                     ggTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGga                                                                                                                     >  1:702805/1‑137 (MQ=255)                     ggTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGga                                                                                                                     <  2:702805/137‑1 (MQ=255)                     ggTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGaa                                                                                                                  >  2:1518948/1‑140 (MQ=255)                     ggTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGaa                                                                                                                  >  2:103255/1‑140 (MQ=255)                       tGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAata                                                                                                                <  1:1308809/140‑1 (MQ=255)                       tGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAata                                                                                                                <  1:2259004/140‑1 (MQ=255)                        gACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGTCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAatat                                                                                                               <  2:1234310/140‑1 (MQ=255)                        gACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAatat                                                                                                               <  1:1518948/140‑1 (MQ=255)                        gACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAatat                                                                                                               <  2:827597/140‑1 (MQ=255)                         aCCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATg                                                                                                              <  2:1978373/140‑1 (MQ=255)                         aCCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATg                                                                                                              >  2:957554/1‑140 (MQ=255)                            aCGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATgggg                                                                                                           <  2:900070/140‑1 (MQ=255)                            aCGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATgggg                                                                                                           <  1:552705/140‑1 (MQ=255)                             cGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATggggg                                                                                                          <  1:2113784/140‑1 (MQ=255)                               ccAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGAt                                                                                                        <  1:103255/140‑1 (MQ=255)                                   tAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCAcc                                                                                                    >  1:1192444/1‑140 (MQ=255)                                   tAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCAcc                                                                                                    >  2:1234318/1‑140 (MQ=255)                                   tAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCAcc                                                                                                    >  2:488011/1‑140 (MQ=255)                                     ggCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAg                                                                                                  <  1:488011/140‑1 (MQ=255)                                      gCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGa                                                                                                 >  2:911100/1‑140 (MQ=255)                                       cAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGAc                                                                                                <  2:1767751/140‑1 (MQ=255)                                          ctctGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCaa                                                                                             >  1:142024/1‑140 (MQ=255)                                          ctctGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCaa                                                                                             >  1:640977/1‑140 (MQ=255)                                            ctGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATa                                                                                           <  2:1935085/140‑1 (MQ=255)                                               cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCAc                                                                                                     <  2:1204469/127‑1 (MQ=255)                                               cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCAc                                                                                                     >  1:1204469/1‑127 (MQ=255)                                               cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAg                                                                                        <  1:1386957/140‑1 (MQ=255)                                                   aGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGa                                                                                    <  1:1141833/140‑1 (MQ=255)                                                     cTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGa                                                                                    >  1:1027221/1‑138 (MQ=255)                                                     cTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGa                                                                                    <  2:1027221/138‑1 (MQ=255)                                                       aaTGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGc                                                                                >  2:580088/1‑140 (MQ=255)                                                             aGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCCCCAGACCAATAAAGCCGATAGCCCCCGc                                                                          <  2:842764/140‑1 (MQ=255)                                                             aGGCGCGCTACAATATCGGCCAGAAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                          <  1:572911/140‑1 (MQ=255)                                                                cgcgcTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAg                                                                       <  1:957554/140‑1 (MQ=255)                                                                cgcgcTAAAATATGGCCCAGCAGCAATGCGCTCGCCCCGGCCAGCGCGCAGCGGGGAAGTAATACGCGATGATCTGTAAACCCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAg                                                                       <  2:2278414/140‑1 (MQ=255)                                                                       cAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCcaca                                                                <  1:580088/140‑1 (MQ=255)                                                                           atCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGa                                                            >  1:1488434/1‑140 (MQ=255)                                                                            tCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGAc                                                           <  1:1202448/140‑1 (MQ=255)                                                                             cGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACg                                                          >  1:1214583/1‑140 (MQ=255)                                                                               gCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGcc                                                        >  2:57680/1‑140 (MQ=255)                                                                               gCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGcc                                                        >  2:164332/1‑140 (MQ=255)                                                                               gCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGcc                                                        >  2:343986/1‑140 (MQ=255)                                                                                    cagcaATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCa                                                   >  1:394975/1‑140 (MQ=255)                                                                                         aTGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAg                                              >  1:1478578/1‑140 (MQ=255)                                                                                          tGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGc                                             >  1:65044/1‑140 (MQ=255)                                                                                                 gCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCgctgtc                                      >  1:2096585/1‑135 (MQ=255)                                                                                                 gCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGc                                      >  1:1198196/1‑140 (MQ=255)                                                                                                           gcgcgcAGCCGGGAAGTAATACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGc                            >  2:1197622/1‑140 (MQ=255)                                                                                                                             aTACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACcac          >  2:216791/1‑140 (MQ=255)                                                                                                                             aTACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACcac          >  2:2034426/1‑140 (MQ=255)                                                                                                                             aTACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACcac          >  1:1614136/1‑140 (MQ=255)                                                                                                                              tACGCGATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACcaca         <  1:164332/140‑1 (MQ=255)                                                                                                                                cgcgATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAgg       >  2:100017/1‑140 (MQ=255)                                                                                                                                 gcgATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAggg      >  1:234839/1‑140 (MQ=255)                                                                                                                                  cgATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAgggg     >  2:2226168/1‑140 (MQ=255)                                                                                                                                  cgATGATCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAgggg     <  1:911100/140‑1 (MQ=255)                                                                                                                                   gatgatCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGt    >  1:187504/1‑140 (MQ=255)                                                                                                                                    atgatCTGTTAAACCACCCAACCGGAGAATAGGGGGGACCACCAGACAAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTa   <  2:2179054/140‑1 (MQ=255)                                                                                                                                     tgatCTGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTaa  >  1:82624/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTAA  >  NC_000913/1794206‑1794477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑