Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,923	G→T	A36S (GCT→TCT)	yecF →	DUF2594 domain‑containing protein YecF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,923	0	G	T	100.0%	350.8 / NA	99	A36S (GCT→TCT)	yecF	DUF2594 domain‑containing protein YecF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/60);  total (39/60)

TTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAAGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGA  >  NC_000913/1995790‑1996057                                                                                                                                      |                                                                                                                                       tttCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGAcg                                                                                                                                  <  1:1429639/140‑1 (MQ=255)     aTTGCAACATGACGAGGAAAGTAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCggg                                                                                                                              <  2:1539407/140‑1 (MQ=255)     aTTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCggg                                                                                                                              <  1:1864854/140‑1 (MQ=255)     aTTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCggg                                                                                                                              <  1:1115466/140‑1 (MQ=255)     aTTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCggg                                                                                                                              <  1:369921/140‑1 (MQ=255)       tGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGcc                                                                                                                            >  1:1819116/1‑140 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  2:252520/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  2:190566/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  2:192466/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  2:1305088/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  2:1273636/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  1:668838/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  1:273724/140‑1 (MQ=255)          aaCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCcgcg                                                                                                                         <  2:206539/140‑1 (MQ=255)           aCATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGt                                                                                                                        <  2:477118/140‑1 (MQ=255)            cATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTg                                                                                                                       <  2:1978741/140‑1 (MQ=255)                 cGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGtt                                                                                                                  <  2:2104875/140‑1 (MQ=255)                   aGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTaa                                                                                                                >  2:1752822/1‑140 (MQ=255)                    ggAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTaaa                                                                                                               <  1:1752822/140‑1 (MQ=255)                        aataaatGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTaaa                                                                                                               >  1:1874876/1‑136 (MQ=255)                        aataaatGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTaaa                                                                                                               <  2:1874876/136‑1 (MQ=255)                             atGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGaaaa                                                                                                      <  1:605759/140‑1 (MQ=255)                               gAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAAc                                                                                                    >  1:1579553/1‑140 (MQ=255)                                aGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTa                                                                                                                 >  1:2001043/1‑126 (MQ=255)                                aGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTa                                                                                                                 <  2:2001043/126‑1 (MQ=255)                                aGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACa                                                                                                   >  1:603374/1‑140 (MQ=255)                                  tACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGc                                                                                                 >  2:1571526/1‑140 (MQ=255)                                  tACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGc                                                                                                 >  1:942497/1‑140 (MQ=255)                                  tACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGc                                                                                                 >  1:810966/1‑140 (MQ=255)                                  tACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGc                                                                                                 >  1:687984/1‑140 (MQ=255)                                  tACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGc                                                                                                 >  1:607310/1‑140 (MQ=255)                                    cGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCtt                                                                                               >  1:2151833/1‑140 (MQ=255)                                     gCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTg                                                                                              <  1:2242974/140‑1 (MQ=255)                                       cTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAACGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTgcg                                                                                            <  2:1878966/140‑1 (MQ=255)                                       cTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTgcg                                                                                            <  2:1579553/140‑1 (MQ=255)                                       cTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTgcg                                                                                            <  2:1819116/140‑1 (MQ=255)                                       cTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTgcg                                                                                            <  2:564608/140‑1 (MQ=255)                                       cTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTgcg                                                                                            <  2:687984/140‑1 (MQ=255)                                          aTTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTg                                                                                         >  1:2050936/1‑140 (MQ=255)                                             tttCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATc                                                                                      <  1:139171/140‑1 (MQ=255)                                              ttCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCg                                                                                     <  2:810966/140‑1 (MQ=255)                                               tCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGa                                                                                    <  2:2151833/140‑1 (MQ=255)                                                   cTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGAc                                                                                <  2:2083240/140‑1 (MQ=255)                                                    tGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACg                                                                               <  1:1512545/140‑1 (MQ=255)                                                     gCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGa                                                                              >  1:34513/1‑140 (MQ=255)                                                     gCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGa                                                                              >  2:516406/1‑140 (MQ=255)                                                                tCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   >  2:594112/1‑140 (MQ=255)                                                                tCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   >  1:2017785/1‑140 (MQ=255)                                                                tCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   <  2:869803/140‑1 (MQ=255)                                                                tCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   >  2:822671/1‑140 (MQ=255)                                                                tCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   >  2:207660/1‑140 (MQ=255)                                                                tCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGACCGAAGACGAAACCCAGGCTg                                                                   >  1:1264539/1‑140 (MQ=255)                                                                  aaGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCa                                                                 <  1:1571526/140‑1 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   >  2:2017341/1‑135 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   <  1:2017341/135‑1 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTa                                                              <  1:594112/140‑1 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTa                                                              >  2:1688523/1‑140 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTa                                                              >  2:2068034/1‑140 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTa                                                              >  2:1332102/1‑140 (MQ=255)                                                                     aaCTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTa                                                              >  1:1532923/1‑140 (MQ=255)                                                                        tGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTAttt                                                           >  2:1022217/1‑140 (MQ=255)                                                                         ggCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCCGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCa                                                                        >  1:774035/1‑126 (MQ=255)                                                                         ggCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCCGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCa                                                                        <  2:774035/126‑1 (MQ=255)                                                                          gCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   >  2:1577928/1‑130 (MQ=255)                                                                          gCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTg                                                                   <  1:1577928/130‑1 (MQ=255)                                                                          gCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTc                                                         >  1:1072744/1‑140 (MQ=255)                                                                          gCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTc                                                         >  2:2248010/1‑140 (MQ=255)                                                                              aTGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCaaa                                                     <  1:1332102/140‑1 (MQ=255)                                                                              aTGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGACCGAAGACGAAACCCAGGCTGCAGTATTTTCCaaa                                                     <  2:1264539/140‑1 (MQ=255)                                                                               agAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCaaaa                                                    <  1:779019/139‑1 (MQ=255)                                                                                    tctcCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGtt                                               >  2:282646/1‑140 (MQ=255)                                                                                      tcCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTaa                                             <  1:1688523/140‑1 (MQ=255)                                                                                      tcCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTaa                                             <  2:603374/140‑1 (MQ=255)                                                                                       cctgcctgAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAg                                            <  1:2068034/140‑1 (MQ=255)                                                                                       cctgcctgAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAg                                            <  1:516406/140‑1 (MQ=255)                                                                                           cctgAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCaaa                                        >  2:150789/1‑140 (MQ=255)                                                                                             tgAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAAtt                                      >  2:885054/1‑140 (MQ=255)                                                                                              gAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTa                                     >  1:1184523/1‑140 (MQ=255)                                                                                                aaGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTaaa                                   <  2:942497/140‑1 (MQ=255)                                                                                                aaGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTaaa                                   <  1:541461/140‑1 (MQ=255)                                                                                                  gCGATGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACa                                 <  1:431569/140‑1 (MQ=255)                                                                                                     aTGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGc                              <  2:2017785/140‑1 (MQ=255)                                                                                                     aTGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGc                              >  2:1201293/1‑140 (MQ=255)                                                                                                     aTGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGc                              <  2:607310/140‑1 (MQ=255)                                                                                                     aTGCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGc                              <  1:529149/140‑1 (MQ=255)                                                                                                     aTGCTGACGCTGATGCTGCAGGCGATGGAACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGc                              >  2:2083735/1‑140 (MQ=255)                                                                                                       gCTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCt                            <  2:1532923/140‑1 (MQ=255)                                                                                                        cTGACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTa                           <  2:2050936/140‑1 (MQ=255)                                                                                                          gACGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTAcc                         <  1:1201293/140‑1 (MQ=255)                                                                                                            cGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGt                       <  2:1849522/140‑1 (MQ=255)                                                                                                            cGCTGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGt                       <  2:1347078/140‑1 (MQ=255)                                                                                                               tGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAg                    <  1:885054/140‑1 (MQ=255)                                                                                                               tGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAg                    <  1:2083735/140‑1 (MQ=255)                                                                                                               tGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAg                    <  2:798833/140‑1 (MQ=255)                                                                                                               tGATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAg                    <  2:34513/140‑1 (MQ=255)                                                                                                                gATGCTGCAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGt                   >  2:1097699/1‑140 (MQ=255)                                                                                                                       cAGGCGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATaa            >  1:1079888/1‑140 (MQ=255)                                                                                                                           cGATGGGACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAAcc        >  2:1395023/1‑140 (MQ=255)                                                                                                                                 gACAATCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGa  <  1:215410/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TTTCATTGCAACATGACGAGGAAAATAAATGAGTACGCCTGATTTTTCTACTGCCGAGAATAATCAAGAACTGGCAAATGAAGTCTCCTGCCTGAAAGCGATGCTGACGCTGATGCTGCAGGCGATGGGACAAGCTGACGCGGGCCGCGTGATGTTAAAGATGGAAAAACAGCTTGCGCTGATCGAAGACGAAACCCAGGCTGCAGTATTTTCCAAAACGGTTAAGCAAATTAAACAAGCCTACCGTCAGTAATATAAAACCGGCTGA  >  NC_000913/1995790‑1996057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑