Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4440TA26.5% 163.5 / 27.9 83intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (8/53);  new base A (22/0);  total (30/53)
Fisher's exact test for biased strand distribution p-value = 8.46e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTA  >  NC_000913/3206308‑3206582
                                                                                                                                         |                                                                                                                                          
gaTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACa                                                                                                                                         <  1:1858176/139‑1 (MQ=255)
   tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACaaaa                                                                                                                                      <  1:1937118/140‑1 (MQ=255)
    cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAaaaaaaaaaa                                                                                                                                     >  2:488033/1‑140 (MQ=255)
    cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGAATTTTTTAGACAaaaaaaaaaa                                                                                                                                     >  2:1556412/1‑140 (MQ=255)
    cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAaaaaaaaaaa                                                                                                                                     >  2:293871/1‑140 (MQ=255)
     ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAAc                                                                                                                                    <  1:293871/140‑1 (MQ=255)
       tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTTGTAGTTTGTGGCGGC‑GAATTTTTCCAGACAAATACaaaaaata                                                                                                                                  >  1:1140737/1‑137 (MQ=255)
         ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                <  1:1895291/140‑1 (MQ=255)
          tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                               <  1:861872/140‑1 (MQ=255)
          tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                               <  2:1933918/140‑1 (MQ=255)
          tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                               <  2:1488687/140‑1 (MQ=255)
          tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                               <  1:488033/140‑1 (MQ=255)
            tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTTAGACAAATACAAAAAAAGGAGtt                                                                                                                             >  2:1895291/1‑140 (MQ=255)
            tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGAGtt                                                                                                                             >  2:1858176/1‑140 (MQ=255)
             ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑GAATTTTTTCAGACAAATACAAAAAATGGAGGTg                                                                                                                            >  2:850696/1‑140 (MQ=255)
               cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAACTGGAGTTGcc                                                                                                                          >  1:1511082/1‑140 (MQ=255)
                gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa                                                                                                                         <  1:1103441/140‑1 (MQ=255)
                 cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGGTGccct                                                                                                                        >  2:1463883/1‑138 (MQ=255)
                   atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCatga                                                                                                                      >  2:1677528/1‑140 (MQ=255)
                   atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                                      <  2:1511082/140‑1 (MQ=255)
                       tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                  <  2:1621946/140‑1 (MQ=255)
                        cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                                 <  2:1802559/140‑1 (MQ=255)
                         attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                <  1:571289/140‑1 (MQ=255)
                          ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                               <  2:1767376/140‑1 (MQ=255)
                           tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGCgga                                                                                                              >  2:692473/1‑138 (MQ=255)
                              tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCC‑TACAAAAAAAAAAAAAAGAGTTGCCATGATTAGCGaaaaa                                                                                                           >  2:531996/1‑138 (MQ=255)
                              tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCAACAAAAAAAAAAAAAGGAGTTGCCATGATTAGCGgaaaa                                                                                                           >  1:864171/1‑138 (MQ=255)
                              tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATAAAAAAAAAGGGGGTGCCCTGATGAGCGAAAga                                                                                                           >  1:1751990/1‑139 (MQ=255)
                              tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGACAAATAAAAAAAATGGAGGTGCCATGATGAGGGaaaaa                                                                                                           >  2:30354/1‑138 (MQ=255)
                                 tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                        <  2:335374/140‑1 (MQ=255)
                                 tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                        <  2:967757/140‑1 (MQ=255)
                                     gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTTTGACAAATAAAAAAAATAGAGTTGCCATGATGAGAGTGAGTAAAAAGc                                                                                                    >  1:1832634/1‑140 (MQ=255)
                                      cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                   <  2:416054/140‑1 (MQ=255)
                                       gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                  <  1:1516694/140‑1 (MQ=255)
                                        ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                 <  2:412510/140‑1 (MQ=255)
                                         tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                <  1:588543/140‑1 (MQ=255)
                                           aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                              <  2:156464/140‑1 (MQ=255)
                                           aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                              <  1:30354/140‑1 (MQ=255)
                                           aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                              <  2:2003328/140‑1 (MQ=255)
                                           aTTCACGAACCGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                              <  1:1368187/140‑1 (MQ=255)
                                            ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                             <  1:1677528/140‑1 (MQ=255)
                                            ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                             <  2:1672756/140‑1 (MQ=255)
                                            ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                             <  1:531996/140‑1 (MQ=255)
                                            ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                             <  1:850696/140‑1 (MQ=255)
                                            ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                             <  1:1698310/140‑1 (MQ=255)
                                                  aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                       <  1:980471/140‑1 (MQ=255)
                                                  aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                       <  2:842509/140‑1 (MQ=255)
                                                  aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                       <  1:1463883/140‑1 (MQ=255)
                                                   aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAAAAAGCAGTGaaa                                                                                      >  1:894945/1‑139 (MQ=255)
                                                     tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGGAAGGAAAAAAAAAAAAGCAGGGAaaaa                                                                                    >  2:131767/1‑140 (MQ=255)
                                                     tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCAACAAAAACAAAAAAAGGAGGTGCCCTGATGAGGGAAAGGAAAAAACAAAAAGCAGTGAaaaa                                                                                    >  2:548433/1‑140 (MQ=255)
                                                      gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                   <  1:1407551/140‑1 (MQ=255)
                                                      gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAg                                                                                   <  1:1439639/140‑1 (MQ=255)
                                                           ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCTGAAAAAAACAAAAAAATGGGTTGGCCTGATGAGCGAAAGAAATAAAAAAAAAGCAGGGAATAAGtttta                                                                              >  2:1291257/1‑137 (MQ=255)
                                                           ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGAAAGAAAAAAAAAAAAAGCAAGGAATAAATTGaa                                                                              >  2:1538661/1‑139 (MQ=255)
                                                               atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAAAAGAGTTGCCATGATGAGCGAAAATAATAAACAAAAAGCAGTGAATAAAATGACagag                                                                          >  2:1417457/1‑140 (MQ=255)
                                                               atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGAGATGGCATGATGAGCGAAAATAAAAAAAAAAAGGAAGAGAAAAAAATGAAagag                                                                          >  2:711521/1‑140 (MQ=255)
                                                                tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                         <  2:256711/140‑1 (MQ=255)
                                                                tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                         <  1:1417457/140‑1 (MQ=255)
                                                                tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                         <  1:501911/140‑1 (MQ=255)
                                                                tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                         <  1:1538661/140‑1 (MQ=255)
                                                                 atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGAGCGAAAAAAAAAAACAACAAGCAGAGAAAAAAATGACAGAGAt                                                                        >  2:249541/1‑140 (MQ=255)
                                                                 atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCTGATGAGCGGAAAAAATAAACAAAAGGCAGTGAAAAAATTGACAGAGAt                                                                        >  2:501911/1‑140 (MQ=255)
                                                                 atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGTCATGATGAGCGAAAGAAAAAAACAAAAAGCAGTGAATAAATTGACAgagaa                                                                        >  2:676129/1‑139 (MQ=255)
                                                                     aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCCGACAAAAAAAAAAAAAGGAGTTGGCCTGATGAGGGAAAATAAAAAAAAACAAGCAGGGAAAAAATTGACAAAGATTGTc                                                                    >  2:1972304/1‑140 (MQ=255)
                                                                      cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGACAAAAAAAAAAAATGGGGGTGCCATGATGAGCGAAAGGAAAAAACAAAAAGCAGGGAAAAAATTGAAAGAGATTGTCg                                                                   >  2:753602/1‑140 (MQ=255)
                                                                        gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCCTGATGAGGGGAAAGAATAAACAAAAAGCAGTGAAAAAATTGACAGAGAATGTTGCt                                                                 >  2:875942/1‑140 (MQ=255)
                                                                        gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                 <  1:1541730/140‑1 (MQ=255)
                                                                         tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAAAAGAGGTGGCCTGATGAGCGGAAGAAATAAACAAAAAGCAGGGAAAAAAATGACAGAGATTGTCGCTa                                                                >  2:700646/1‑140 (MQ=255)
                                                                            cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACCAAAAAAAAAAATGGAGTTGCCCTGATGAGGGGAAGAAATAAACAACAGGCAGTGAAAAAATTGAAAGAGATAGTCGCTaaat                                                             >  2:215323/1‑138 (MQ=255)
                                                                            cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGAAAAAAACAAAAAGACGTGAAAAAATTGAAAGAGAATGTCGCTaaat                                                             >  2:1298450/1‑138 (MQ=255)
                                                                                aaaTATAAAACCCCATTTTTAGTGGTATTTTGTGGCGGT‑GATTTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAACCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTAc                                                         <  2:1832634/140‑1 (MQ=255)
                                                                                 aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                        <  1:692473/140‑1 (MQ=255)
                                                                                         aCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                                <  1:875942/140‑1 (MQ=255)
                                                                                         aCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                                <  2:894945/140‑1 (MQ=255)
                                                                                             caTTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAGACAAAAACAAAAAAAGGAGGTGCCATGATGAGCGAAAGAAATAAACAACAAGCAGGGAATAAGATGACAGAGATTGTCGCTAACTTTACCGCCATGAttttt                                            >  2:1741234/1‑138 (MQ=255)
                                                                                                  ttAGTGGTAGTTTGTGGCGGT‑GATTTTTTCCAGAAAAATAAAAAACCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                                       <  1:711521/140‑1 (MQ=255)
                                                                                                   tAGTGGTAGTTTGTGGCGGT‑AATTTTTTCCAGACAAATAAAAAAACTGGAGTTCCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                      <  2:250721/140‑1 (MQ=255)
                                                                                                    aGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGaa                                     <  1:580855/140‑1 (MQ=255)
                                                                                                    aGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                     <  1:676129/140‑1 (MQ=255)
                                                                                                        gTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAAATGGGGGTGGCATGATGAGGGGAAAGAATAAAAAAAAAGCAGTGAAAAAGTTTACAGAGAATTTTGCTAAATTTTACCCCATGATTTTTTCCCCAATGcc                                 >  2:1285129/1‑140 (MQ=255)
                                                                                                        gTAGTTTGTGGCGGT‑GAATTTTCCCAGACAAATAGAAAAACGGGAGTTGCCATGAGGAGCAAAATGAATAGGCAACAGTGAGGGAATAAGTGGACAGAGTTTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGcc                                 <  2:2014907/140‑1 (MQ=255)
                                                                                                         tAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                                <  1:1298450/140‑1 (MQ=255)
                                                                                                             ttGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                            <  1:1285129/140‑1 (MQ=255)
                                                                                                                 ggCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                        <  1:753602/140‑1 (MQ=255)
                                                                                                                  gCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                       <  1:1675504/140‑1 (MQ=255)
                                                                                                                   gggT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg                      <  1:548433/139‑1 (MQ=255)
                                                                                                                     gT‑GAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGGGAAAGTAATAAACAAAAAGCAGTGAAAAAATTGACAGAGATTGTCGCTAACTTTACCCCCCTGATTTTTTCCCCCATGCCTGATGACgggggg                    >  2:222014/1‑138 (MQ=255)
                                                                                                                        gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                  <  1:700646/140‑1 (MQ=255)
                                                                                                                        aattttttCCAGACAAATACAAAAACTGGAGTTGCCATGAGGAGGAAAAGTAATAAGCAACAGGAAGTGAATAATTTGACAGAGATTGGCGCTAACTTATCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                  <  2:1751990/137‑1 (MQ=255)
                                                                                                                               tCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa           <  1:1984426/140‑1 (MQ=255)
                                                                                                                                  aaaCAAATACAAAACCTGGAGTTGCAAGGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGGCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa        <  2:1140737/138‑1 (MQ=255)
                                                                                                                                  aGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa        <  1:1972304/140‑1 (MQ=255)
                                                                                                                                  aGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa        <  1:131767/140‑1 (MQ=255)
                                                                                                                                   gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                       <  2:585522/124‑1 (MQ=255)
                                                                                                                                   gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                       >  1:585522/1‑124 (MQ=255)
                                                                                                                                   gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc       >  1:1613768/1‑140 (MQ=255)
                                                                                                                                        aTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa  <  1:1741234/140‑1 (MQ=255)
                                                                                                                                        aTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa  >  1:2068614/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTA  >  NC_000913/3206308‑3206582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑