Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	31.5%	132.5 / 40.2	73	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (34/16);  new base C (0/23);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.04e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTG  >  NC_000913/445423‑445693                                                                                                                                        |                                                                                                                                        aaaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGGCCGCATTGCGCCAGCGAGAAAAAaccc                                                                                                                                     >  2:457792/1‑140 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGGCTGTCAGCCCCACTGCGGCCCGCATTGCGCCAGCGAGAAAAAcccccc                                                                                                                                   >  2:2393031/1‑139 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:741761/140‑1 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:930348/140‑1 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:1978920/140‑1 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:1680341/140‑1 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:2028054/140‑1 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  1:1447101/140‑1 (MQ=255)   aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                   <  2:2044120/140‑1 (MQ=255)          tttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGGACTGGCGGCTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTc                                                                                                                            >  2:741761/1‑140 (MQ=255)               cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACa                                                                                                                       <  1:728699/140‑1 (MQ=255)               cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACa                                                                                                                       <  2:948316/140‑1 (MQ=255)                gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                      <  1:457792/140‑1 (MQ=255)                gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                      <  2:1013589/140‑1 (MQ=255)                gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                      <  2:725979/140‑1 (MQ=255)                gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCCACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCTCTGACCGTCAAACAt                                                                                                                      <  2:2385222/140‑1 (MQ=255)                     gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATTGGACTGGCGACTGTCAGCCACACTGCGGGCAGCATTTCGCCCGCGAGAAATACCCCCCGACCCGCAAACATGCCGt                                                                                                                 >  2:561584/1‑140 (MQ=255)                     gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCCGCGGGAAATAACCCCTGACCGGCAAACATGCCGt                                                                                                                 >  2:1313141/1‑140 (MQ=255)                                   tCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGccc                                                                                                   <  2:783235/140‑1 (MQ=255)                                    cGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTTCGCCCGCGAGAAAAAACCCCCGACCCTCAAAAATGCCGGTAATCCCGccccccc                                                                                                  >  2:494128/1‑139 (MQ=255)                                    cGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCCAATGTCCGCCACACTTCGGGCCGCCTTTCGCCCGCGAGAAATAACCCCCTACCCGCAAAAATACCGTTCATTCAGCCGcccc                                                                                                  >  2:1211654/1‑139 (MQ=255)                                           aTTTCAATGCGCAAACTGCTGACATACGGCGGCTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAAcc                                                                                           >  1:884415/1‑140 (MQ=255)                                                    cgcAAACTGCTGACATACGGCGGTTCTTTCATTGTACAGGCGGCTGTCAACCCCACTACGGCCCGCACTGCGCCCGCGAGAAAAACCCCCCCACCGCCAAAAAACCCCTTAATCCCACCCCCCAGCGAACCGCCCATccc                                                                                  >  2:1786390/1‑138 (MQ=255)                                                    cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                  <  1:1388281/140‑1 (MQ=255)                                                      aaaaCTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                                <  1:494128/139‑1 (MQ=255)                                                              tgACATACGGCGGTTCTTTCATGGTACGGGCGACTGTCAGCCACACTGCGGCCAGCATGGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGa                                                                        <  2:998779/140‑1 (MQ=255)                                                                aCATCCGGCGTTTCTTTCATGGTACTGGCGCCTGTCAGCCACACTGGGGCCGGCATTGCGCCAGCGAGAAATCCCCCCGGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                      <  2:2150829/140‑1 (MQ=255)                                                                   tACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGCGGGAAAAACCCCCCGACCCTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCCATCGCCCCGCCCAGAAAatg                                                                   >  2:1446166/1‑140 (MQ=255)                                                                    ccggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActgg                                                                  <  1:1313141/139‑1 (MQ=255)                                                                     cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                 <  1:990198/140‑1 (MQ=255)                                                                     cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                                 >  1:1160125/1‑140 (MQ=255)                                                                     cggcggTTCTTTCATGGTACCGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAAACGCCAATCGCCACGCCCAGAAActggc                                                                 >  2:2399993/1‑140 (MQ=255)                                                                       gcggTTTTTTTATGGTACTGGCGACCGTCAGCCACACTGCGGGCAGCAATGCGCCAGCGAGAAAAACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGGACCGCCAATCGCCACCCCCAGAAActggctg                                                               >  2:678657/1‑140 (MQ=255)                                                                           ttCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtgg                                                           <  1:1624248/140‑1 (MQ=255)                                                                              tttCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATGGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGt                                                        <  1:1207450/140‑1 (MQ=255)                                                                                    ggTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAacac                                                  >  2:997794/1‑140 (MQ=255)                                                                                     gtccgggCGACTGTCAGCCACACGGCGGCAAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                 <  2:1122437/135‑1 (MQ=255)                                                                                     gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                 >  1:2420761/1‑140 (MQ=255)                                                                                      tACGGGCGACTGTCAGCCACACTGCGGCCAGCATTGCCCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCAAGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                                <  2:963255/140‑1 (MQ=255)                                                                                       aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                               >  1:2002796/1‑140 (MQ=255)                                                                                       aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                               >  1:1574502/1‑140 (MQ=255)                                                                                        cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCATCCGACCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                              >  2:2188349/1‑140 (MQ=255)                                                                                        cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                              >  2:1921699/1‑140 (MQ=255)                                                                                        cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                              >  2:1636637/1‑140 (MQ=255)                                                                                        cTGGCGACTGTCAACCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                              >  2:1223336/1‑140 (MQ=255)                                                                                          ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                            <  1:780716/140‑1 (MQ=255)                                                                                          ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                            >  2:1547463/1‑140 (MQ=255)                                                                                          ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                            >  1:1208598/1‑140 (MQ=255)                                                                                          ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCAAGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                            >  1:963255/1‑140 (MQ=255)                                                                                           gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                           >  1:1706112/1‑140 (MQ=255)                                                                                            cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGTCAAGCGAACAGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGAAAAGACCCATCGcc                                          >  2:613759/1‑140 (MQ=255)                                                                                            cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                          >  1:639490/1‑140 (MQ=255)                                                                                            cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                          >  1:740557/1‑140 (MQ=255)                                                                                              ccTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                        <  2:884415/139‑1 (MQ=255)                                                                                              ccTGCCACCCACACGGCGGCAAGCATGGCGCAAGCAAGAAATCCCCCCTGCCCGTAAAAAATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                        <  2:668583/139‑1 (MQ=255)                                                                                              aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                        <  1:1290786/140‑1 (MQ=255)                                                                                               ttttcggCCACACGGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                       <  2:1885051/134‑1 (MQ=255)                                                                                               cTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                       <  1:1399716/140‑1 (MQ=255)                                                                                                tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  1:1897980/1‑140 (MQ=255)                                                                                                tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  1:1209778/1‑140 (MQ=255)                                                                                                tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  2:843575/1‑140 (MQ=255)                                                                                                tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                      >  2:91720/1‑140 (MQ=255)                                                                                                  tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                    >  2:2216712/1‑140 (MQ=255)                                                                                                    aGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCttt                                  >  1:1273045/1‑140 (MQ=255)                                                                                                    aGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCttt                                  >  1:1687110/1‑140 (MQ=255)                                                                                                     gCCACACTGCGGCCAGCATTGTGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                 >  2:2219968/1‑140 (MQ=255)                                                                                                          ccTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAcc                            <  1:360571/139‑1 (MQ=255)                                                                                                            tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          >  2:1680208/1‑140 (MQ=255)                                                                                                            tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          >  1:81685/1‑140 (MQ=255)                                                                                                            ggCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                          <  1:2399993/139‑1 (MQ=255)                                                                                                              cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                        >  1:1143195/1‑140 (MQ=255)                                                                                                              cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                        >  2:1996840/1‑140 (MQ=255)                                                                                                                gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                      >  2:237940/1‑140 (MQ=255)                                                                                                                   aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                   <  1:91720/140‑1 (MQ=255)                                                                                                                   aGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                   >  2:1832398/1‑140 (MQ=255)                                                                                                                    gctttGCCCCGGCGAGAAATCCCCCCTGCCCGTAAACCATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                  <  2:2420761/137‑1 (MQ=255)                                                                                                                     cATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                 <  1:1996840/140‑1 (MQ=255)                                                                                                                     cATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                 <  1:1636637/140‑1 (MQ=255)                                                                                                                     cATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                 >  1:2480729/1‑140 (MQ=255)                                                                                                                       ttGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct               <  1:1446166/140‑1 (MQ=255)                                                                                                                       ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct               <  1:843575/140‑1 (MQ=255)                                                                                                                       ttGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct               <  1:4861/140‑1 (MQ=255)                                                                                                                       tggcgcCAGGGAAAAACCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct               <  2:2002796/138‑1 (MQ=255)                                                                                                                       tggccccAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct               <  2:1749912/135‑1 (MQ=255)                                                                                                                         gcgcCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt             <  1:678657/140‑1 (MQ=255)                                                                                                                         gcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt             <  1:997794/140‑1 (MQ=255)                                                                                                                         gcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt             <  1:1547463/140‑1 (MQ=255)                                                                                                                         ccgcCAGCGAAAAACCCCCCCTGCCCGACAACCGTGCCGTTAACCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt             <  2:1706112/139‑1 (MQ=255)                                                                                                                          cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa            >  2:1233385/1‑140 (MQ=255)                                                                                                                          cgcCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTAAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa            <  2:950244/140‑1 (MQ=255)                                                                                                                             cAGCGAAAAACCCCCCCTGCCCGAAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTg         <  2:1160125/140‑1 (MQ=255)                                                                                                                               gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt       >  2:1187537/1‑140 (MQ=255)                                                                                                                               gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACC‑TTGTAACCTGCTGGCGACTCTTTACTGATa      >  1:1894996/1‑140 (MQ=255)                                                                                                                                   gaAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt   <  1:1223336/140‑1 (MQ=255)                                                                                                                                   aaaaTCCCCCCTGCCCGTAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt   <  2:171277/139‑1 (MQ=255)                                                                                                                                   aaaaTCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt   <  1:2216712/139‑1 (MQ=255)                                                                                                                                   aaaaTCCCCCCCACCCGTCAAACATGCCGTTAACCCAGCCGCCAAGCGATCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt   <  2:243364/139‑1 (MQ=255)                                                                                                                                    aaaTACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg  <  1:1832398/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTG  >  NC_000913/445423‑445693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑