BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,836	G→T	L507L (CTG→CTT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,836	0	G	T	99.4%	595.0 / ‑3.9	165	L507L (CTG→CTT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (88/76); total (88/77)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

CGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCC  >  NC_000913/3932706‑3932972

 gCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTgc                                                                                                                                >  1:117048/1‑140 (MQ=255)

  cTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTgcg                                                                                                                               >  2:2134154/1‑140 (MQ=255)

         aaTCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGaa                                                                                                                        <  2:525122/140‑1 (MQ=255)

         aaTCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTACATGAGCGGGTGATTCTTTTAACTCTGCGCACCGaa                                                                                                                        <  2:332852/140‑1 (MQ=255)

             aCCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACg                                                                                                                    <  1:2369978/140‑1 (MQ=255)

             aCCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACg                                                                                                                    >  2:673229/1‑140 (MQ=255)

             aCCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACg                                                                                                                    <  1:1957428/140‑1 (MQ=255)

               cGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGa                                                                                                                      >  1:962027/1‑136 (MQ=255)

               cGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCt                                                                                                                  >  2:945982/1‑140 (MQ=255)

                gCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTc                                                                                                                 >  1:827152/1‑140 (MQ=255)

                gCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTc                                                                                                                 <  2:438519/140‑1 (MQ=255)

                   tGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCat                                                                                                              <  2:1550198/140‑1 (MQ=255)

                   tGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAAAATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCat                                                                                                              <  1:1185233/140‑1 (MQ=255)

                    gTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCata                                                                                                             <  1:194957/140‑1 (MQ=255)

                    gTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCata                                                                                                             <  1:851995/140‑1 (MQ=255)

                    gTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCata                                                                                                             <  2:1274976/140‑1 (MQ=255)

                      tCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGc                                                                                                                   >  1:841280/1‑132 (MQ=255)

                      tCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGc                                                                                                                   <  2:841280/132‑1 (MQ=255)

                      tCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATg                                                                                                           <  2:419547/140‑1 (MQ=255)

                                gggACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgt                                                                                                 <  1:1356628/140‑1 (MQ=255)

                                   aCCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccg                                                                                              <  1:2168439/140‑1 (MQ=255)

                                    ccGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccgt                                                                                             <  1:570778/140‑1 (MQ=255)

                                    ccGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccgt                                                                                             <  1:1442399/140‑1 (MQ=255)

                                            tataTGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACa                                                                                     <  1:475161/140‑1 (MQ=255)

                                                 gTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccgt                                                                                             <  2:372975/127‑1 (MQ=255)

                                                 gTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAcgtccgt                                                                                             >  1:372975/1‑127 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGa                                                                                   <  1:226728/134‑1 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             <  2:1983802/140‑1 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  1:2425104/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  1:2215363/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  2:2160738/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  2:1260918/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  1:1001268/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  2:2242068/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  1:2065209/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  1:1909495/1‑140 (MQ=255)

                                                    gcgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaac                                                                             >  1:1872344/1‑140 (MQ=255)

                                                     cgTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaaca                                                                            >  1:1261804/1‑140 (MQ=255)

                                                      gTGCAATCAACGTCATTCCCTTTGCGCTGATGTATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaacaa                                                                           >  1:1106872/1‑140 (MQ=255)

                                                      gTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaacaa                                                                           >  2:1913087/1‑140 (MQ=255)

                                                      gTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaacaa                                                                           >  2:1560362/1‑140 (MQ=255)

                                                      gTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaacaa                                                                           >  2:479403/1‑140 (MQ=255)

                                                      gTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaacaa                                                                           <  2:1026163/140‑1 (MQ=255)

                                                      gTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGaacaa                                                                           <  1:1843995/140‑1 (MQ=255)

                                                             cAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGc                                                                    <  2:827152/140‑1 (MQ=255)

                                                             cAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGc                                                                    <  2:265759/140‑1 (MQ=255)

                                                                cGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCa                                                                 <  1:1260918/140‑1 (MQ=255)

                                                                     ttCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTc                                                            >  2:29804/1‑140 (MQ=255)

                                                                        ccTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTgg                                                         >  2:1148485/1‑140 (MQ=255)

                                                                         cTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGgc                                                        <  1:28620/140‑1 (MQ=255)

                                                                          tttGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGgcg                                                       >  1:789918/1‑140 (MQ=255)

                                                                            tGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGcgcg                                                     >  2:509356/1‑140 (MQ=255)

                                                                            tGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGcgcg                                                     <  1:29804/140‑1 (MQ=255)

                                                                             gcgcTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAGCTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCgt                                                    <  2:2365676/140‑1 (MQ=255)

                                                                              cgcTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTGCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCgtg                                                   <  2:2454641/140‑1 (MQ=255)

                                                                              cgcTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCgtg                                                   <  1:20914/140‑1 (MQ=255)

                                                                              cgcTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCgtg                                                   <  2:2215363/140‑1 (MQ=255)

                                                                               gcTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGT‑‑CCCACTTTCTGGCGCgtggtg                                                <  1:2160738/140‑1 (MQ=255)

                                                                                   aTGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGc                                              <  2:1157289/140‑1 (MQ=255)

                                                                                   aTGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGc                                              <  1:1094809/140‑1 (MQ=255)

                                                                                   aTGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGc                                              <  1:2242068/140‑1 (MQ=255)

                                                                                        tAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGtt                                         >  1:2316083/1‑140 (MQ=255)

                                                                                            cTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATgg                                     <  1:785466/140‑1 (MQ=255)

                                                                                             ttAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGt                                    <  2:2280823/140‑1 (MQ=255)

                                                                                              tAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGtt                                   >  1:2326909/1‑140 (MQ=255)

                                                                                              tAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGtt                                   >  1:2196305/1‑140 (MQ=255)

                                                                                              tAAACATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGtt                                   >  2:219499/1‑140 (MQ=255)

                                                                                               aaaCATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTg                                  <  2:291865/140‑1 (MQ=255)

                                                                                                aaCATAACAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTgg                                 >  2:2426142/1‑140 (MQ=255)

                                                                                                aaCATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTgg                                 <  1:1175633/140‑1 (MQ=255)

                                                                                                     aaCAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCgaga                            >  2:182995/1‑140 (MQ=255)

                                                                                                     aaCAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCgaga                            >  1:1688567/1‑140 (MQ=255)

                                                                                                     aaCAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCgaga                            >  2:337603/1‑140 (MQ=255)

                                                                                                      aCAAGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaa                           <  2:1106872/140‑1 (MQ=255)

                                                                                                         aGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACg                        <  2:1042001/140‑1 (MQ=255)

                                                                                                         aGGTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACg                        >  2:93123/1‑140 (MQ=255)

                                                                                                          ggTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGc                       <  1:509356/140‑1 (MQ=255)

                                                                                                          ggTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGc                       >  2:2293147/1‑140 (MQ=255)

                                                                                                          ggTATTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGc                       <  2:1398722/140‑1 (MQ=255)

                                                                                                             aTTGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCaa                    >  2:2270846/1‑140 (MQ=255)

                                                                                                              ttGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCaaa                   <  2:789918/140‑1 (MQ=255)

                                                                                                               tGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                  >  1:2013405/1‑140 (MQ=255)

                                                                                                               tGCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                  >  2:461407/1‑140 (MQ=255)

                                                                                                                gCATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACg                 <  1:93123/140‑1 (MQ=255)

                                                                                                                 cATGAGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGt                >  1:557068/1‑140 (MQ=255)

                                                                                                                     aGCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa            >  2:802667/1‑140 (MQ=255)

                                                                                                                      gCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag           >  1:301504/1‑140 (MQ=255)

                                                                                                                      gCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag           >  2:832022/1‑140 (MQ=255)

                                                                                                                      gCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag           >  1:887709/1‑140 (MQ=255)

                                                                                                                      gCGGGTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag           >  1:1146469/1‑140 (MQ=255)

                                                                                                                      gCGGGTGATTCTTTTAACTCTGCGCACCGAAG‑CGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaaga          >  1:1493955/1‑140 (MQ=255)

                                                                                                                         ggTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag        >  1:1010018/1‑140 (MQ=255)

                                                                                                                         ggTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag        >  2:1189757/1‑140 (MQ=255)

                                                                                                                         ggTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag        >  1:2466383/1‑140 (MQ=255)

                                                                                                                         ggTGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag        >  2:1811516/1‑140 (MQ=255)

                                                                                                                           tGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtt      >  1:45069/1‑140 (MQ=255)

                                                                                                                           tGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtt      >  2:1092644/1‑140 (MQ=255)

                                                                                                                           tGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtt      >  2:1957838/1‑140 (MQ=255)

                                                                                                                           tGATTCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtt      >  2:1881948/1‑140 (MQ=255)

                                                                                                                               tCTTTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTcc  >  2:87285/1‑140 (MQ=255)

CGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATGTCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTATTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCC  >  NC_000913/3932706‑3932972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`