BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,471,990	G→T	P136T (CCG→ACG)	pyrB ←	aspartate carbamoyltransferase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,471,990	0	G	T	100.0%	543.2 / NA	154	P136T (CCG→ACG)	pyrB	aspartate carbamoyltransferase catalytic subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (80/74); total (80/74)

Only 100 of 156 total aligned reads displayed.

TCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTA  >  NC_000913/4471854‑4472123

tCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATg                                                                                                                                    <  1:76210/140‑1 (MQ=255)

  gAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATgtt                                                                                                                                  <  2:2020701/140‑1 (MQ=255)

     cTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATgttggt                                                                                                                               <  1:43815/140‑1 (MQ=255)

              cGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCAt                                                                                                                      <  2:285153/140‑1 (MQ=255)

                ccTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCa                                                                                                                    >  1:1301158/1‑140 (MQ=255)

                  tGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCAcc                                                                                                                  >  1:1580472/1‑140 (MQ=255)

                  tGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCAcc                                                                                                                  >  1:168797/1‑140 (MQ=255)

                   gAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCg                                                                                                                 <  2:2082519/140‑1 (MQ=255)

                     gTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCAt                                                                                                                      >  2:1254059/1‑133 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCa                                                                                                              <  1:2070620/137‑1 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTc                                                                                                           <  1:393523/140‑1 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTc                                                                                                           <  2:2054274/140‑1 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTc                                                                                                           <  2:2271201/140‑1 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTc                                                                                                           <  2:1448709/140‑1 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTc                                                                                                           <  2:643624/140‑1 (MQ=255)

                         gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTc                                                                                                           <  2:669302/140‑1 (MQ=255)

                           gAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAg                                                                                                         <  2:589041/140‑1 (MQ=255)

                           gAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAg                                                                                                         <  2:1716366/140‑1 (MQ=255)

                              tGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTAc                                                                                                      >  1:1317698/1‑140 (MQ=255)

                                aaCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGc                                                                                                               >  2:2395845/1‑129 (MQ=255)

                                aaCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCg                                                                                                    >  1:1816959/1‑140 (MQ=255)

                                 aCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCgg                                                                                                   <  1:1719576/140‑1 (MQ=255)

                                     tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTAc                                                                                                      >  1:870770/1‑133 (MQ=255)

                                     tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTAc                                                                                                      <  2:870770/133‑1 (MQ=255)

                                        ggCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAg                                                                                                         <  2:406515/127‑1 (MQ=255)

                                         gCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGc                                                                                           >  1:589617/1‑140 (MQ=255)

                                                   aGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCCGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTc                                                                                 >  1:1672594/1‑140 (MQ=255)

                                                        accaaccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTgg                                                                            >  1:2183170/1‑140 (MQ=255)

                                                        accaaccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTgg                                                                            >  2:2104141/1‑140 (MQ=255)

                                                            accaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAg                                                                        <  1:2300238/140‑1 (MQ=255)

                                                             ccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCCGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAgg                                                                       <  2:1672594/140‑1 (MQ=255)

                                                               aTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcg                                                                     >  2:489351/1‑140 (MQ=255)

                                                               aTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcg                                                                     <  2:290556/140‑1 (MQ=255)

                                                               aTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcg                                                                     >  1:2313535/1‑140 (MQ=255)

                                                                ttGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcgc                                                                    >  2:2362879/1‑140 (MQ=255)

                                                                ttGCGACGCGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcgc                                                                    >  2:1813988/1‑140 (MQ=255)

                                                                 tGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcgcg                                                                   >  2:1579606/1‑140 (MQ=255)

                                                                   cGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGcgcc                                                                 <  2:1991279/140‑1 (MQ=255)

                                                                    gACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGcgccg                                                                >  2:1022032/1‑140 (MQ=255)

                                                                     aCGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGcgccgc                                                               >  2:1603143/1‑140 (MQ=255)

                                                                     aCGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGcgccgc                                                               >  1:2402480/1‑140 (MQ=255)

                                                                      cGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa                                                              <  2:827400/140‑1 (MQ=255)

                                                                       gTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAc                                                             >  1:949343/1‑140 (MQ=255)

                                                                        tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                            >  2:2042983/1‑140 (MQ=255)

                                                                        tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                            <  2:2210473/140‑1 (MQ=255)

                                                                        tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                            >  2:769661/1‑140 (MQ=255)

                                                                        tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                            >  1:1356640/1‑140 (MQ=255)

                                                                        tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                            >  1:1448637/1‑140 (MQ=255)

                                                                        tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc                                                            >  1:174349/1‑140 (MQ=255)

                                                                          gagaTTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGcc                                                                          <  2:1497084/124‑1 (MQ=255)

                                                                          gagaTTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGcc                                                                          >  1:1497084/1‑124 (MQ=255)

                                                                            gaTTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTcc                                                        <  2:174349/140‑1 (MQ=255)

                                                                                  ccAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa                                                  <  1:2306178/140‑1 (MQ=255)

                                                                                  ccAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa                                                  <  2:1449626/140‑1 (MQ=255)

                                                                                  ccAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa                                                  <  2:2009056/140‑1 (MQ=255)

                                                                                       cGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACg                                             <  1:2362879/140‑1 (MQ=255)

                                                                                             tGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCAc                                       <  2:1816959/140‑1 (MQ=255)

                                                                                             tGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCAc                                       <  1:202373/140‑1 (MQ=255)

                                                                                                gTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTAt                                    <  1:384481/140‑1 (MQ=255)

                                                                                                   tCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGc                                 >  2:770568/1‑140 (MQ=255)

                                                                                                   tCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGc                                 <  2:589617/140‑1 (MQ=255)

                                                                                                    ccTGAGTAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCa                                >  1:261633/1‑140 (MQ=255)

                                                                                                    ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCa                                >  2:1171156/1‑140 (MQ=255)

                                                                                                    ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCa                                >  1:2016222/1‑140 (MQ=255)

                                                                                                    ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCa                                >  1:684400/1‑140 (MQ=255)

                                                                                                    ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCa                                >  2:940773/1‑140 (MQ=255)

                                                                                                      tGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATc                              <  1:1171156/140‑1 (MQ=255)

                                                                                                         aTAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGAc                           >  2:916835/1‑140 (MQ=255)

                                                                                                         aTAGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGAc                           >  1:1246140/1‑140 (MQ=255)

                                                                                                           aGTGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGt                         <  2:980428/140‑1 (MQ=255)

                                                                                                             tGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCAt                               >  1:735288/1‑132 (MQ=255)

                                                                                                             tGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa                       >  2:1905402/1‑140 (MQ=255)

                                                                                                             tGAATAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa                       >  1:287498/1‑140 (MQ=255)

                                                                                                               aaTAAGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGt                         <  2:221643/136‑1 (MQ=255)

                                                                                                                  aaGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCt                  >  1:2043939/1‑140 (MQ=255)

                                                                                                                   aGTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTg                 <  1:1285978/140‑1 (MQ=255)

                                                                                                                    gTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGa                >  1:206590/1‑140 (MQ=255)

                                                                                                                    gTCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCCACGTAAGTGCTGa                >  2:2304885/1‑140 (MQ=255)

                                                                                                                     tCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGAt               <  1:162654/140‑1 (MQ=255)

                                                                                                                     tCCAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGAt               <  2:2061717/140‑1 (MQ=255)

                                                                                                                       cAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa                       >  2:703178/1‑130 (MQ=255)

                                                                                                                       cAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa                       <  1:703178/130‑1 (MQ=255)

                                                                                                                       cAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATaa             >  1:1078708/1‑140 (MQ=255)

                                                                                                                       cAGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATaa             >  2:1471762/1‑140 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  1:1471762/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  1:2042983/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  1:1055607/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  2:1078708/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  2:261633/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  2:2317787/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  2:2313535/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  2:167189/140‑1 (MQ=255)

                                                                                                                        aGCAAGGTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc            <  2:1744407/140‑1 (MQ=255)

                                                                                                                             ggTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa       <  1:916835/140‑1 (MQ=255)

                                                                                                                             ggTTTGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa       <  2:2306042/140‑1 (MQ=255)

                                                                                                                               tttGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATg     >  1:2354066/1‑140 (MQ=255)

                                                                                                                               tttGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATg     >  2:244431/1‑140 (MQ=255)

                                                                                                                                ttGCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATgg    <  1:2254853/140‑1 (MQ=255)

                                                                                                                                  gCGTCGTATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTa  <  2:307484/140‑1 (MQ=255)

                                                                                                                                   cgtcgtATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGAt               >  2:1167560/1‑126 (MQ=255)

TCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTA  >  NC_000913/4471854‑4472123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`